Vibrio phage VcP032

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Longwoodvirus; Vibrio virus K139

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DHU2|A0A160DHU2_9CAUD Uncharacterized protein OS=Vibrio phage VcP032 OX=1841196 GN=VcP032_09 PE=4 SV=1
MM1 pKa = 7.33AALYY5 pKa = 10.07KK6 pKa = 10.17IEE8 pKa = 4.59SYY10 pKa = 10.99SDD12 pKa = 3.04EE13 pKa = 4.2AARR16 pKa = 11.84QIGGFITEE24 pKa = 3.94HH25 pKa = 6.68GGRR28 pKa = 11.84CVVAGFAVITDD39 pKa = 3.76HH40 pKa = 7.52LFQQGDD46 pKa = 3.65AFQVLPLVARR56 pKa = 11.84TSDD59 pKa = 3.75QLSEE63 pKa = 3.36WDD65 pKa = 3.74YY66 pKa = 11.41QQFSYY71 pKa = 11.44

Molecular weight: 7.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160DHH9|A0A160DHH9_9CAUD Uncharacterized protein OS=Vibrio phage VcP032 OX=1841196 GN=VcP032_34 PE=4 SV=1
MM1 pKa = 7.44FEE3 pKa = 4.32IKK5 pKa = 10.75AEE7 pKa = 3.96DD8 pKa = 3.47RR9 pKa = 11.84SYY11 pKa = 11.38LRR13 pKa = 11.84VMEE16 pKa = 3.87QLEE19 pKa = 4.31LLGLDD24 pKa = 3.01RR25 pKa = 11.84KK26 pKa = 8.46TRR28 pKa = 11.84DD29 pKa = 2.86KK30 pKa = 9.91MLRR33 pKa = 11.84RR34 pKa = 11.84IGAQIAKK41 pKa = 5.88TTRR44 pKa = 11.84KK45 pKa = 9.87NIRR48 pKa = 11.84AQRR51 pKa = 11.84DD52 pKa = 3.4PDD54 pKa = 3.34GSAWAKK60 pKa = 9.84RR61 pKa = 11.84KK62 pKa = 9.77RR63 pKa = 11.84GRR65 pKa = 11.84GKK67 pKa = 10.15LLKK70 pKa = 10.86GFTQKK75 pKa = 10.56LKK77 pKa = 10.79HH78 pKa = 5.53FQRR81 pKa = 11.84DD82 pKa = 3.38NNRR85 pKa = 11.84TLVVGWPSARR95 pKa = 11.84GRR97 pKa = 11.84VAYY100 pKa = 8.84EE101 pKa = 3.64HH102 pKa = 6.53HH103 pKa = 6.93HH104 pKa = 6.91GIAQEE109 pKa = 4.31SGLSARR115 pKa = 11.84KK116 pKa = 9.03RR117 pKa = 11.84QAKK120 pKa = 7.72QQNEE124 pKa = 3.87PRR126 pKa = 11.84KK127 pKa = 7.6TDD129 pKa = 3.41PATRR133 pKa = 11.84EE134 pKa = 3.63QAKK137 pKa = 10.28ALRR140 pKa = 11.84DD141 pKa = 3.05LDD143 pKa = 4.12YY144 pKa = 11.01RR145 pKa = 11.84LKK147 pKa = 10.49PQGRR151 pKa = 11.84QKK153 pKa = 10.55RR154 pKa = 11.84GKK156 pKa = 9.91KK157 pKa = 7.83PTLAWIRR164 pKa = 11.84EE165 pKa = 3.88NMTVGEE171 pKa = 4.07AAKK174 pKa = 9.6TIQEE178 pKa = 4.61LEE180 pKa = 4.22NKK182 pKa = 8.94TPARR186 pKa = 11.84DD187 pKa = 3.43WKK189 pKa = 10.37VGRR192 pKa = 11.84PEE194 pKa = 3.7RR195 pKa = 11.84RR196 pKa = 11.84LIGISPKK203 pKa = 10.28RR204 pKa = 11.84LAIMIKK210 pKa = 10.2RR211 pKa = 11.84EE212 pKa = 4.14LKK214 pKa = 10.4RR215 pKa = 11.84NRR217 pKa = 11.84SKK219 pKa = 11.5

Molecular weight: 25.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10386

39

861

225.8

25.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.234 ± 0.552

1.127 ± 0.188

5.536 ± 0.226

7.212 ± 0.38

3.871 ± 0.23

6.047 ± 0.315

1.974 ± 0.19

6.21 ± 0.268

6.278 ± 0.37

8.627 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.821 ± 0.24

4.333 ± 0.21

4.198 ± 0.259

5.065 ± 0.246

5.074 ± 0.371

6.364 ± 0.265

5.536 ± 0.29

6.027 ± 0.268

1.56 ± 0.126

2.908 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski