Klebsiella phage F19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; Klebsiella virus F19

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9QJC1|V9QJC1_9CAUD Uncharacterized protein OS=Klebsiella phage F19 OX=1416011 GN=F19_11 PE=4 SV=1
MM1 pKa = 7.26VNVFNIIVTSAMLVLGNDD19 pKa = 3.45VNNPVPYY26 pKa = 9.36CTVQLQQPAAQEE38 pKa = 3.83PLPRR42 pKa = 11.84PEE44 pKa = 3.99YY45 pKa = 10.94DD46 pKa = 3.92LFEE49 pKa = 6.59DD50 pKa = 4.29PDD52 pKa = 4.42GGCKK56 pKa = 9.79EE57 pKa = 3.86LGARR61 pKa = 11.84ILEE64 pKa = 4.5AVQEE68 pKa = 4.3QYY70 pKa = 11.34PDD72 pKa = 3.32AAVTLTVDD80 pKa = 3.9GKK82 pKa = 11.4SNNDD86 pKa = 2.72II87 pKa = 4.2

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9QKJ4|V9QKJ4_9CAUD Uncharacterized protein OS=Klebsiella phage F19 OX=1416011 GN=F19_12 PE=4 SV=1
MM1 pKa = 7.37HH2 pKa = 7.34GKK4 pKa = 9.42NPEE7 pKa = 3.94TLLMRR12 pKa = 11.84KK13 pKa = 7.33QQPTIEE19 pKa = 4.25GLARR23 pKa = 11.84EE24 pKa = 4.45YY25 pKa = 10.62SAKK28 pKa = 10.43AALRR32 pKa = 11.84QHH34 pKa = 6.15YY35 pKa = 7.59EE36 pKa = 3.85KK37 pKa = 10.33QAKK40 pKa = 8.74RR41 pKa = 11.84LGMTLRR47 pKa = 11.84GYY49 pKa = 10.27CYY51 pKa = 9.33RR52 pKa = 11.84FNVRR56 pKa = 11.84GVII59 pKa = 3.65

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

13234

43

1239

259.5

28.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.624 ± 0.51

1.118 ± 0.191

6.219 ± 0.237

5.811 ± 0.387

3.159 ± 0.141

7.602 ± 0.26

1.919 ± 0.219

4.504 ± 0.206

4.783 ± 0.302

8.743 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.871 ± 0.201

3.914 ± 0.246

3.876 ± 0.205

4.632 ± 0.35

5.932 ± 0.266

6.362 ± 0.382

5.569 ± 0.297

7.133 ± 0.242

1.436 ± 0.115

3.793 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski