Chuzan virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; Palyam virus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9YN53|Q9YN53_9REOV Core protein VP7 OS=Chuzan virus OX=77204 PE=3 SV=1
MM1 pKa = 7.99GDD3 pKa = 3.25RR4 pKa = 11.84KK5 pKa = 10.39RR6 pKa = 11.84YY7 pKa = 6.62TKK9 pKa = 8.78TVCVYY14 pKa = 10.47DD15 pKa = 3.78PQNATFCSRR24 pKa = 11.84GANAGEE30 pKa = 4.3NYY32 pKa = 9.56YY33 pKa = 9.23QVKK36 pKa = 9.83IGRR39 pKa = 11.84TTQITRR45 pKa = 11.84TNAPNPKK52 pKa = 9.53AYY54 pKa = 10.04VVEE57 pKa = 4.19IQRR60 pKa = 11.84PCSVRR65 pKa = 11.84LLDD68 pKa = 4.22GNDD71 pKa = 3.47IISLIISEE79 pKa = 4.45EE80 pKa = 4.42GIEE83 pKa = 4.23VTTEE87 pKa = 3.6RR88 pKa = 11.84WEE90 pKa = 3.97EE91 pKa = 3.82WLFEE95 pKa = 4.3ALTPIPMVISLHH107 pKa = 6.2IKK109 pKa = 8.04GQKK112 pKa = 8.28TEE114 pKa = 4.83AEE116 pKa = 3.88IKK118 pKa = 8.4YY119 pKa = 9.28CKK121 pKa = 10.13ASGVIAPYY129 pKa = 9.47TPNGVDD135 pKa = 3.8RR136 pKa = 11.84RR137 pKa = 11.84VTPQLPGITFTDD149 pKa = 3.5VSVRR153 pKa = 11.84DD154 pKa = 4.16FRR156 pKa = 11.84QKK158 pKa = 9.75MRR160 pKa = 11.84EE161 pKa = 3.78EE162 pKa = 3.94RR163 pKa = 11.84EE164 pKa = 4.03KK165 pKa = 11.01EE166 pKa = 4.04KK167 pKa = 10.85EE168 pKa = 4.07RR169 pKa = 11.84TPMITPIKK177 pKa = 8.79PKK179 pKa = 10.52SEE181 pKa = 4.03APMMGPSTSQQYY193 pKa = 9.77QKK195 pKa = 10.81EE196 pKa = 3.96EE197 pKa = 4.22SIAVGKK203 pKa = 10.64ISDD206 pKa = 3.93YY207 pKa = 10.39VKK209 pKa = 10.76KK210 pKa = 9.63VTLEE214 pKa = 4.23SNVEE218 pKa = 3.89RR219 pKa = 11.84DD220 pKa = 3.28SDD222 pKa = 4.61DD223 pKa = 3.7EE224 pKa = 4.9DD225 pKa = 3.65EE226 pKa = 5.26HH227 pKa = 8.59GSNSDD232 pKa = 3.04SDD234 pKa = 4.28FDD236 pKa = 5.32GEE238 pKa = 4.58PKK240 pKa = 10.67DD241 pKa = 4.01EE242 pKa = 5.34GYY244 pKa = 8.26ITQNYY249 pKa = 7.55MNMIEE254 pKa = 4.27TVKK257 pKa = 10.43MKK259 pKa = 10.74VPGVAGLTLKK269 pKa = 10.35IPKK272 pKa = 9.8AAGKK276 pKa = 7.75TDD278 pKa = 2.97GMIAAKK284 pKa = 8.99KK285 pKa = 9.14CKK287 pKa = 9.11WISVPLFNIDD297 pKa = 4.7KK298 pKa = 10.78DD299 pKa = 3.78GLSYY303 pKa = 11.49DD304 pKa = 4.15FVAVGDD310 pKa = 4.25ANGFLCLRR318 pKa = 11.84DD319 pKa = 3.5GLSYY323 pKa = 11.06LVLPVSGSVHH333 pKa = 5.85

Molecular weight: 37.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A109RY65|A0A109RY65_9REOV Minor core protein OS=Chuzan virus OX=77204 PE=4 SV=1
MM1 pKa = 7.71LARR4 pKa = 11.84SLNEE8 pKa = 3.83YY9 pKa = 10.46KK10 pKa = 10.9AMRR13 pKa = 11.84PEE15 pKa = 4.3SEE17 pKa = 4.2MSVVPYY23 pKa = 9.82QPPAYY28 pKa = 6.96PTAPTGTNGEE38 pKa = 4.36KK39 pKa = 10.56DD40 pKa = 3.67FSSISLGVLNNAMNDD55 pKa = 3.34TTAATQAEE63 pKa = 4.48RR64 pKa = 11.84EE65 pKa = 4.28EE66 pKa = 4.31KK67 pKa = 10.1VAYY70 pKa = 10.26ASFAEE75 pKa = 4.11ALRR78 pKa = 11.84DD79 pKa = 3.77SACVRR84 pKa = 11.84EE85 pKa = 4.06IKK87 pKa = 10.45KK88 pKa = 10.18RR89 pKa = 11.84VSSRR93 pKa = 11.84TIVALEE99 pKa = 3.9KK100 pKa = 10.41EE101 pKa = 4.55YY102 pKa = 11.23NHH104 pKa = 6.24QKK106 pKa = 10.38RR107 pKa = 11.84IYY109 pKa = 10.54DD110 pKa = 4.42FIRR113 pKa = 11.84LILFIMSVIAIITSSLSAAIVVIPEE138 pKa = 4.18TKK140 pKa = 10.03ALKK143 pKa = 10.33EE144 pKa = 3.82EE145 pKa = 4.56WISILIHH152 pKa = 5.96VGNLFATGVFVGMSKK167 pKa = 10.23YY168 pKa = 10.7AEE170 pKa = 4.2RR171 pKa = 11.84LDD173 pKa = 4.11SFLKK177 pKa = 9.42RR178 pKa = 11.84TRR180 pKa = 11.84KK181 pKa = 10.09EE182 pKa = 3.7IVKK185 pKa = 9.75KK186 pKa = 10.51RR187 pKa = 11.84SYY189 pKa = 10.75IEE191 pKa = 4.04AANVTWDD198 pKa = 3.81SDD200 pKa = 3.46VDD202 pKa = 3.71TLNFLKK208 pKa = 10.82AAAA211 pKa = 4.4

Molecular weight: 23.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6071

211

1295

607.1

69.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.111 ± 0.632

1.219 ± 0.214

6.078 ± 0.258

6.968 ± 0.445

4.414 ± 0.368

5.732 ± 0.483

2.026 ± 0.207

7.478 ± 0.25

5.93 ± 0.646

8.285 ± 0.435

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.624 ± 0.301

4.167 ± 0.26

3.838 ± 0.293

3.821 ± 0.311

6.951 ± 0.336

6.473 ± 0.299

5.222 ± 0.396

6.342 ± 0.217

1.268 ± 0.236

4.052 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski