Simian foamy virus Pongo pygmaeus pygmaeus
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7SIS5|Q7SIS5_9RETR Env leader protein OS=Simian foamy virus Pongo pygmaeus pygmaeus OX=221703 GN=env PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 5.82 PNQEE6 pKa = 4.07 EE7 pKa = 4.61 EE8 pKa = 4.48 PVAGTSGMNQDD19 pKa = 4.06 PVPFIPEE26 pKa = 4.72 GIAAANEE33 pKa = 4.18 SDD35 pKa = 4.84 DD36 pKa = 4.31 EE37 pKa = 4.3 EE38 pKa = 5.71 PEE40 pKa = 3.89 QFLYY44 pKa = 10.37 KK45 pKa = 10.36 VYY47 pKa = 10.62 QEE49 pKa = 4.31 SVKK52 pKa = 10.86 KK53 pKa = 10.8 NGGDD57 pKa = 3.68 YY58 pKa = 10.54 PKK60 pKa = 10.86 LEE62 pKa = 4.25 DD63 pKa = 4.39 WIPSPEE69 pKa = 3.99 EE70 pKa = 3.62 MSKK73 pKa = 10.46 SVCISLILTCLYY85 pKa = 10.26 NAEE88 pKa = 4.2 KK89 pKa = 10.37 AAQIKK94 pKa = 8.87 DD95 pKa = 2.84 WGYY98 pKa = 9.42 IVHH101 pKa = 6.92 WEE103 pKa = 3.96 QSPTDD108 pKa = 3.57 SKK110 pKa = 11.74 YY111 pKa = 10.73 FLIKK115 pKa = 10.02 YY116 pKa = 6.86 EE117 pKa = 4.74 CPMCDD122 pKa = 4.54 SINQEE127 pKa = 4.56 PMPIWWDD134 pKa = 3.39 DD135 pKa = 3.4 RR136 pKa = 11.84 LKK138 pKa = 10.64 LWRR141 pKa = 11.84 KK142 pKa = 6.36 MGCRR146 pKa = 11.84 AVMGSIVYY154 pKa = 9.91 ALKK157 pKa = 10.91 NHH159 pKa = 5.68 VDD161 pKa = 3.17 KK162 pKa = 11.38 CNSQVHH168 pKa = 5.96 PLRR171 pKa = 11.84 KK172 pKa = 8.01 TGNRR176 pKa = 11.84 RR177 pKa = 11.84 PRR179 pKa = 11.84 PRR181 pKa = 11.84 IDD183 pKa = 3.95 PIRR186 pKa = 11.84 RR187 pKa = 11.84 CNRR190 pKa = 11.84 LTGNYY195 pKa = 8.92 VPGRR199 pKa = 11.84 RR200 pKa = 11.84 GSTKK204 pKa = 9.48 PSNPSSHH211 pKa = 7.06 PSSGIPLAPGPRR223 pKa = 11.84 QCSTNTSNPPEE234 pKa = 4.17 SLLRR238 pKa = 11.84 LLPGNDD244 pKa = 4.0 AISPALAISMSGGQIWEE261 pKa = 4.04 EE262 pKa = 4.5 VYY264 pKa = 11.4 NDD266 pKa = 4.21 LLLDD270 pKa = 3.93 ATLGTSDD277 pKa = 3.25 NN278 pKa = 4.01
Molecular weight: 31.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.172
IPC2_protein 5.308
IPC_protein 5.232
Toseland 5.207
ProMoST 5.397
Dawson 5.258
Bjellqvist 5.372
Wikipedia 5.143
Rodwell 5.181
Grimsley 5.143
Solomon 5.258
Lehninger 5.219
Nozaki 5.397
DTASelect 5.55
Thurlkill 5.245
EMBOSS 5.207
Sillero 5.461
Patrickios 3.935
IPC_peptide 5.27
IPC2_peptide 5.461
IPC2.peptide.svr19 5.474
Protein with the highest isoelectric point:
>tr|Q7SIS7|Q7SIS7_9RETR Gag polyprotein OS=Simian foamy virus Pongo pygmaeus pygmaeus OX=221703 GN=gag PE=4 SV=1
MM1 pKa = 7.61 AAQNFEE7 pKa = 4.86 LDD9 pKa = 3.77 VQEE12 pKa = 5.2 LLNLFQDD19 pKa = 3.68 NGVTRR24 pKa = 11.84 NPRR27 pKa = 11.84 HH28 pKa = 6.44 LEE30 pKa = 4.23 TIGLRR35 pKa = 11.84 MLGGWWGEE43 pKa = 3.78 QEE45 pKa = 4.58 RR46 pKa = 11.84 YY47 pKa = 8.37 QSARR51 pKa = 11.84 IILQDD56 pKa = 4.13 DD57 pKa = 3.93 DD58 pKa = 5.43 GEE60 pKa = 4.28 PLQVPRR66 pKa = 11.84 WEE68 pKa = 4.18 EE69 pKa = 3.35 VLRR72 pKa = 11.84 PVNPLAHH79 pKa = 6.14 FVISAPWDD87 pKa = 3.31 QLRR90 pKa = 11.84 RR91 pKa = 11.84 AFHH94 pKa = 6.97 DD95 pKa = 3.82 LDD97 pKa = 3.8 VGNGALRR104 pKa = 11.84 FGPLANGNYY113 pKa = 9.41 IPEE116 pKa = 4.63 DD117 pKa = 4.24 PYY119 pKa = 9.74 STSYY123 pKa = 11.02 RR124 pKa = 11.84 PVNPQEE130 pKa = 3.91 MAQMQRR136 pKa = 11.84 DD137 pKa = 3.7 EE138 pKa = 4.6 LEE140 pKa = 4.14 EE141 pKa = 3.84 VLEE144 pKa = 4.17 VQGEE148 pKa = 4.35 IEE150 pKa = 4.38 LQMIDD155 pKa = 4.71 LIEE158 pKa = 4.2 MQTIEE163 pKa = 4.6 IRR165 pKa = 11.84 GLRR168 pKa = 11.84 QLVNEE173 pKa = 4.6 LQRR176 pKa = 11.84 EE177 pKa = 4.02 RR178 pKa = 11.84 DD179 pKa = 3.49 SGRR182 pKa = 11.84 GASIPGASSSPPPQSYY198 pKa = 10.69 VGLPGMPSVSGLSSLQPEE216 pKa = 4.57 ASSTPGGRR224 pKa = 11.84 APRR227 pKa = 11.84 VSFHH231 pKa = 7.03 PSNPFVRR238 pKa = 11.84 PPSPEE243 pKa = 3.61 RR244 pKa = 11.84 PRR246 pKa = 11.84 ARR248 pKa = 11.84 SSEE251 pKa = 4.42 RR252 pKa = 11.84 IPLPLQPPVIQYY264 pKa = 9.9 VPVPQPVLAPAPAPSPVIPIQHH286 pKa = 5.76 IRR288 pKa = 11.84 AVTGEE293 pKa = 4.25 VPNNPRR299 pKa = 11.84 DD300 pKa = 3.38 IPMWIGRR307 pKa = 11.84 NAPAIEE313 pKa = 4.1 GVYY316 pKa = 10.33 PVTTPDD322 pKa = 2.9 LRR324 pKa = 11.84 ARR326 pKa = 11.84 IINALIGGKK335 pKa = 9.78 SGIHH339 pKa = 4.87 LTAPEE344 pKa = 4.03 AVTWASAVAAIFTRR358 pKa = 11.84 THH360 pKa = 6.36 GSFPMHH366 pKa = 6.49 NLSAILTGIANGEE379 pKa = 4.37 GVEE382 pKa = 4.23 SAYY385 pKa = 11.12 NLGMMLSNGDD395 pKa = 3.52 FNLVYY400 pKa = 10.51 GIVRR404 pKa = 11.84 GLLPGQAAVAYY415 pKa = 6.92 MQQRR419 pKa = 11.84 LDD421 pKa = 4.24 AEE423 pKa = 4.42 PSDD426 pKa = 4.09 ALRR429 pKa = 11.84 AQNFIQHH436 pKa = 6.11 LHH438 pKa = 5.71 LVYY441 pKa = 10.58 EE442 pKa = 4.33 ILGLNHH448 pKa = 7.38 RR449 pKa = 11.84 GQSIRR454 pKa = 11.84 TSLPTSTRR462 pKa = 11.84 PRR464 pKa = 11.84 GQGRR468 pKa = 11.84 GRR470 pKa = 11.84 GQGQGSIPSTPRR482 pKa = 11.84 RR483 pKa = 11.84 PQSGRR488 pKa = 11.84 GLSTPNRR495 pKa = 11.84 GSNNANNNTQSNVQTEE511 pKa = 4.43 TPRR514 pKa = 11.84 RR515 pKa = 11.84 SFGGYY520 pKa = 7.35 NLRR523 pKa = 11.84 PNTFRR528 pKa = 11.84 PQRR531 pKa = 11.84 YY532 pKa = 9.31 GGGQGQRR539 pKa = 11.84 RR540 pKa = 11.84 DD541 pKa = 3.68 SQPDD545 pKa = 3.03 RR546 pKa = 11.84 RR547 pKa = 11.84 SQGSSQNNRR556 pKa = 11.84 PSAPLEE562 pKa = 4.04 SRR564 pKa = 11.84 GEE566 pKa = 3.96 QSRR569 pKa = 11.84 GPGGGGRR576 pKa = 11.84 AGGRR580 pKa = 11.84 RR581 pKa = 11.84 NQNRR585 pKa = 11.84 NSGQGNEE592 pKa = 4.35 SSSHH596 pKa = 5.48 AVNAVTQSAVTEE608 pKa = 4.12 QQNEE612 pKa = 4.63 SPTPPPTSGGRR623 pKa = 11.84 SS624 pKa = 3.24
Molecular weight: 67.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.852
IPC2_protein 9.136
IPC_protein 10.248
Toseland 9.399
ProMoST 9.575
Dawson 9.823
Bjellqvist 9.706
Wikipedia 10.175
Rodwell 9.721
Grimsley 9.984
Solomon 9.897
Lehninger 9.809
Nozaki 9.37
DTASelect 9.706
Thurlkill 9.589
EMBOSS 9.867
Sillero 9.736
Patrickios 5.041
IPC_peptide 9.867
IPC2_peptide 8.829
IPC2.peptide.svr19 8.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3357
278
1145
671.4
75.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.541 ± 0.631
1.549 ± 0.64
4.349 ± 0.448
5.749 ± 0.505
2.532 ± 0.198
6.136 ± 1.284
2.562 ± 0.322
6.673 ± 0.858
5.809 ± 1.34
9.89 ± 0.679
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.906 ± 0.286
5.451 ± 0.249
7.417 ± 0.869
5.451 ± 0.489
5.004 ± 1.158
6.494 ± 0.611
5.898 ± 0.62
5.839 ± 0.48
1.906 ± 0.241
3.843 ± 0.509
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here