Beihai sea slater virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF04|A0A1L3KF04_9VIRU RNA-directed RNA polymerase OS=Beihai sea slater virus 3 OX=1922659 PE=4 SV=1
MM1 pKa = 7.62AFLIPALVGGIEE13 pKa = 3.92AAVAGGGAVLGNKK26 pKa = 8.94LAHH29 pKa = 6.36KK30 pKa = 10.53VVGDD34 pKa = 4.18APADD38 pKa = 3.44LEE40 pKa = 4.76GIMEE44 pKa = 4.26VMQFLDD50 pKa = 3.8PSQNPSSMPRR60 pKa = 11.84MLQSQEE66 pKa = 4.28DD67 pKa = 3.94LSSALEE73 pKa = 3.94TRR75 pKa = 11.84LNIQGLDD82 pKa = 3.54NEE84 pKa = 4.76KK85 pKa = 9.24KK86 pKa = 8.89TKK88 pKa = 9.89FVSNLQALKK97 pKa = 10.57GQADD101 pKa = 4.16LALKK105 pKa = 10.56DD106 pKa = 3.82LLRR109 pKa = 11.84LKK111 pKa = 9.46STQGLSDD118 pKa = 3.29ATAVMNQAPNLIQNQPSLTSNPVPQAQVVTSPGLNVQARR157 pKa = 11.84EE158 pKa = 4.25APPTLQTLIQNLQAQAVPQSQVQDD182 pKa = 3.48SSALTRR188 pKa = 11.84NEE190 pKa = 3.66PSTTFSAVYY199 pKa = 9.34KK200 pKa = 9.87YY201 pKa = 10.1PVSRR205 pKa = 11.84LPLQASQPISKK216 pKa = 10.22NVLNQGTVIPRR227 pKa = 11.84PQVEE231 pKa = 4.69GTSSSQGTTNLRR243 pKa = 11.84TTASDD248 pKa = 3.52TVQGVGQALKK258 pKa = 10.68GAASDD263 pKa = 3.57AGKK266 pKa = 10.61VAVTALGNAAAEE278 pKa = 4.29VGKK281 pKa = 10.77GVLSHH286 pKa = 6.96LGKK289 pKa = 10.41LAAAKK294 pKa = 10.24IGDD297 pKa = 3.99FSSILAKK304 pKa = 10.48KK305 pKa = 9.95IVGDD309 pKa = 3.8AVPANDD315 pKa = 3.92HH316 pKa = 7.47RR317 pKa = 11.84IGTWMRR323 pKa = 11.84SEE325 pKa = 4.24KK326 pKa = 10.7VVGDD330 pKa = 4.06AEE332 pKa = 4.79VSTKK336 pKa = 10.94DD337 pKa = 3.55NPKK340 pKa = 10.7LMALLQQMANGGSAPKK356 pKa = 10.37SGEE359 pKa = 3.95DD360 pKa = 3.27KK361 pKa = 10.88TDD363 pKa = 3.15GKK365 pKa = 10.7IVGDD369 pKa = 3.91APPGVMEE376 pKa = 4.71KK377 pKa = 10.17QDD379 pKa = 3.4EE380 pKa = 4.68TMTDD384 pKa = 3.39EE385 pKa = 4.35QVAAAAGNLQSSPSLALMSHH405 pKa = 6.31IQLDD409 pKa = 4.04EE410 pKa = 4.03QMLEE414 pKa = 3.91SGKK417 pKa = 10.24VVEE420 pKa = 4.86FTSDD424 pKa = 2.87VSEE427 pKa = 4.33AARR430 pKa = 11.84MIARR434 pKa = 11.84LGEE437 pKa = 4.12EE438 pKa = 3.88VSGVFKK444 pKa = 9.84TEE446 pKa = 3.66RR447 pKa = 11.84LISTRR452 pKa = 11.84TQVGNQYY459 pKa = 10.05FWNTYY464 pKa = 6.58FTLRR468 pKa = 11.84NPRR471 pKa = 11.84LDD473 pKa = 3.4YY474 pKa = 10.45DD475 pKa = 3.87AKK477 pKa = 10.11VTTEE481 pKa = 4.16SGAEE485 pKa = 4.09VNNGTSNDD493 pKa = 3.22FGYY496 pKa = 10.91LYY498 pKa = 10.76LARR501 pKa = 11.84FHH503 pKa = 6.27MFPVTSVTSIPMLPSATLTTLGPLLSGSSLTEE535 pKa = 3.53YY536 pKa = 11.07ARR538 pKa = 11.84DD539 pKa = 3.5LRR541 pKa = 11.84LTTLSFNYY549 pKa = 10.33DD550 pKa = 4.28LIARR554 pKa = 11.84LWPMIADD561 pKa = 3.4NLEE564 pKa = 3.95NQTGYY569 pKa = 11.22SFIRR573 pKa = 11.84QIIVLLDD580 pKa = 3.85LLCDD584 pKa = 3.77LTGVIPGTEE593 pKa = 3.99YY594 pKa = 11.64GEE596 pKa = 4.28ITSNLYY602 pKa = 8.63VQPTARR608 pKa = 11.84HH609 pKa = 5.79RR610 pKa = 11.84NNEE613 pKa = 3.76NAAVFDD619 pKa = 4.27DD620 pKa = 4.28SGWNAFRR627 pKa = 11.84TEE629 pKa = 3.88RR630 pKa = 11.84VAQADD635 pKa = 3.83GRR637 pKa = 11.84WIKK640 pKa = 10.03FAILDD645 pKa = 3.7EE646 pKa = 4.27VTYY649 pKa = 10.86GKK651 pKa = 10.37VEE653 pKa = 4.04TGNFSTSDD661 pKa = 3.2FDD663 pKa = 6.16GIFTPTNWGKK673 pKa = 10.16NSAIVFVPIQKK684 pKa = 9.1TNDD687 pKa = 3.71FKK689 pKa = 11.12WIAAWTLAHH698 pKa = 7.4LAHH701 pKa = 6.94PWSNYY706 pKa = 8.89GATHH710 pKa = 6.93TYY712 pKa = 8.57ATTRR716 pKa = 11.84EE717 pKa = 4.15NGDD720 pKa = 3.09GYY722 pKa = 11.54AFHH725 pKa = 7.38TDD727 pKa = 3.37PNSNAIFNTNYY738 pKa = 9.83GCALAGPAMGVLYY751 pKa = 10.41VIVGTVKK758 pKa = 10.83LEE760 pKa = 3.7EE761 pKa = 3.93ARR763 pKa = 11.84INFAGRR769 pKa = 11.84VLTGDD774 pKa = 5.42DD775 pKa = 4.28IPPSEE780 pKa = 4.27PFEE783 pKa = 4.59FGDD786 pKa = 4.56NGFDD790 pKa = 3.42DD791 pKa = 5.15YY792 pKa = 11.47YY793 pKa = 11.74NVMVDD798 pKa = 3.3PSITAVCEE806 pKa = 4.45CIRR809 pKa = 11.84FWEE812 pKa = 4.58EE813 pKa = 3.75TFGTASEE820 pKa = 4.37RR821 pKa = 11.84ASAMRR826 pKa = 11.84IVASNRR832 pKa = 11.84KK833 pKa = 9.03CYY835 pKa = 9.97KK836 pKa = 10.35DD837 pKa = 3.5KK838 pKa = 11.61NFMGNGQLPGQVIASDD854 pKa = 4.08KK855 pKa = 10.95YY856 pKa = 9.42STHH859 pKa = 6.01QFATGPKK866 pKa = 8.04IWQGLGNGDD875 pKa = 3.22IANWSDD881 pKa = 3.54TVSSNDD887 pKa = 3.62LLTAQAVLAFGCQNALLMKK906 pKa = 9.89PVLRR910 pKa = 11.84GIRR913 pKa = 11.84SSSAAPVTGEE923 pKa = 4.84SEE925 pKa = 4.62TNLNTQLVFSEE936 pKa = 4.3RR937 pKa = 11.84NPLVEE942 pKa = 5.08LFVLKK947 pKa = 10.72KK948 pKa = 10.49LLTPAEE954 pKa = 4.4EE955 pKa = 4.57YY956 pKa = 10.33PVTRR960 pKa = 11.84LDD962 pKa = 3.26KK963 pKa = 10.54HH964 pKa = 5.88VRR966 pKa = 11.84TVTVLSAMCDD976 pKa = 3.49EE977 pKa = 4.46IAAMVDD983 pKa = 3.86LSCQANSVPSAIACYY998 pKa = 9.66PEE1000 pKa = 5.82LYY1002 pKa = 9.95TEE1004 pKa = 4.44SANICAFVRR1013 pKa = 11.84NKK1015 pKa = 10.05INPICSGILTLGIQFPQVFYY1035 pKa = 11.32ADD1037 pKa = 3.85TEE1039 pKa = 4.7SFVYY1043 pKa = 9.53TLEE1046 pKa = 4.08RR1047 pKa = 11.84ARR1049 pKa = 11.84LLSATINSDD1058 pKa = 3.13NYY1060 pKa = 10.5VNSNYY1065 pKa = 10.52LIGLSRR1071 pKa = 11.84IPTHH1075 pKa = 5.86TVSQYY1080 pKa = 10.78VPSLQDD1086 pKa = 3.04NRR1088 pKa = 11.84TGMQLSADD1096 pKa = 3.8VLRR1099 pKa = 11.84PRR1101 pKa = 11.84LGKK1104 pKa = 10.53FSGLVPNDD1112 pKa = 3.52WEE1114 pKa = 4.46GSTFFSVDD1122 pKa = 3.37SASDD1126 pKa = 3.52NMDD1129 pKa = 3.92DD1130 pKa = 6.17DD1131 pKa = 4.59ISIDD1135 pKa = 3.45NLLRR1139 pKa = 11.84PLSRR1143 pKa = 11.84GVIINSVNATSTNYY1157 pKa = 10.09GFAIASEE1164 pKa = 4.67GGNTAYY1170 pKa = 9.76PVLVHH1175 pKa = 5.74LTSWDD1180 pKa = 3.62FVINTIFTKK1189 pKa = 10.76AMNDD1193 pKa = 3.52PNITIPPGDD1202 pKa = 3.68SGMTPHH1208 pKa = 7.76DD1209 pKa = 3.71EE1210 pKa = 4.58RR1211 pKa = 11.84ILRR1214 pKa = 11.84RR1215 pKa = 11.84VPVDD1219 pKa = 2.84AWRR1222 pKa = 11.84RR1223 pKa = 11.84GRR1225 pKa = 11.84IIMTGPRR1232 pKa = 11.84DD1233 pKa = 3.66LFSPGSGPVYY1243 pKa = 10.22AYY1245 pKa = 11.27SLFSQVNSPSNIQTTKK1261 pKa = 10.3AWNADD1266 pKa = 2.94NDD1268 pKa = 4.19SAYY1271 pKa = 10.51RR1272 pKa = 11.84AADD1275 pKa = 3.41LGTVFKK1281 pKa = 10.26YY1282 pKa = 8.76TT1283 pKa = 3.41

Molecular weight: 138.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF62|A0A1L3KF62_9VIRU Uncharacterized protein OS=Beihai sea slater virus 3 OX=1922659 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 4.99GFFNPSSSRR11 pKa = 11.84GHH13 pKa = 6.38RR14 pKa = 11.84GGSGRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84CSAWKK26 pKa = 9.35QARR29 pKa = 11.84TQSRR33 pKa = 11.84RR34 pKa = 11.84GRR36 pKa = 11.84ASRR39 pKa = 11.84PGRR42 pKa = 11.84DD43 pKa = 3.38NGSDD47 pKa = 3.42AILRR51 pKa = 11.84PKK53 pKa = 10.52SKK55 pKa = 10.45SFKK58 pKa = 9.2HH59 pKa = 5.96ASNASIPRR67 pKa = 11.84RR68 pKa = 11.84LEE70 pKa = 3.33QCIRR74 pKa = 11.84NTIEE78 pKa = 3.59HH79 pKa = 6.15SRR81 pKa = 11.84FGQRR85 pKa = 11.84KK86 pKa = 6.74EE87 pKa = 3.76NKK89 pKa = 9.24IRR91 pKa = 11.84FQPAGVEE98 pKa = 4.41GASGLGVEE106 pKa = 4.99RR107 pKa = 11.84LTQAEE112 pKa = 3.52IDD114 pKa = 3.69ARR116 pKa = 11.84AEE118 pKa = 3.63RR119 pKa = 11.84CYY121 pKa = 10.99CRR123 pKa = 11.84DD124 pKa = 3.3EE125 pKa = 4.94SGTEE129 pKa = 3.99SDD131 pKa = 4.55SEE133 pKa = 3.84PAIIDD138 pKa = 3.98FEE140 pKa = 4.58PSSPGTGGDD149 pKa = 3.08IPGFEE154 pKa = 4.62CAGEE158 pKa = 3.98RR159 pKa = 11.84SSPNPPNIDD168 pKa = 3.79PEE170 pKa = 4.44PTSSGSATEE179 pKa = 4.13PSSGQLSPNTKK190 pKa = 9.76RR191 pKa = 11.84ALDD194 pKa = 3.78NLLSCIQVSSFPPSTASLAAYY215 pKa = 9.94LKK217 pKa = 10.11EE218 pKa = 4.24CVEE221 pKa = 4.46SRR223 pKa = 11.84DD224 pKa = 4.02SNTKK228 pKa = 10.29ASSS231 pKa = 3.0

Molecular weight: 24.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2377

231

1283

792.3

88.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.615 ± 1.487

1.346 ± 0.452

6.31 ± 0.621

4.964 ± 0.599

4.712 ± 0.89

6.353 ± 1.056

1.388 ± 0.181

5.721 ± 0.674

5.385 ± 1.495

8.961 ± 1.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.893 ± 0.366

5.974 ± 0.361

4.838 ± 0.832

3.45 ± 0.79

5.595 ± 1.308

7.993 ± 1.739

6.899 ± 0.687

6.353 ± 1.084

1.388 ± 0.265

2.861 ± 0.452
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski