Soybean yellow mottle mosaic virus
Average proteome isoelectric point is 8.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7U998|B7U998_9TOMB Capsid protein OS=Soybean yellow mottle mosaic virus OX=578361 GN=CP PE=3 SV=1
MM1 pKa = 7.91 PSTRR5 pKa = 11.84 HH6 pKa = 5.19 EE7 pKa = 4.43 VNHH10 pKa = 6.24 LAVITGVILLLWHH23 pKa = 6.71 IRR25 pKa = 11.84 SQSILILIFDD35 pKa = 5.61 AIAPRR40 pKa = 11.84 VTNITSAILLAIFYY54 pKa = 9.79 CYY56 pKa = 10.7 SNSTASIPYY65 pKa = 8.12 ISQPVTHH72 pKa = 6.96 SSTSKK77 pKa = 9.79 IVYY80 pKa = 9.36 IAVGTNPVAVSKK92 pKa = 11.28 HH93 pKa = 4.4 EE94 pKa = 3.9
Molecular weight: 10.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.538
IPC2_protein 8.361
IPC_protein 8.785
Toseland 8.741
ProMoST 8.595
Dawson 8.96
Bjellqvist 8.975
Wikipedia 9.077
Rodwell 8.96
Grimsley 8.726
Solomon 9.341
Lehninger 9.311
Nozaki 8.96
DTASelect 8.887
Thurlkill 8.887
EMBOSS 9.136
Sillero 9.107
Patrickios 3.719
IPC_peptide 9.326
IPC2_peptide 8.185
IPC2.peptide.svr19 7.916
Protein with the highest isoelectric point:
>tr|B7U997|B7U997_9TOMB p10 protein OS=Soybean yellow mottle mosaic virus OX=578361 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 5.63 KK3 pKa = 10.79 SPQRR7 pKa = 11.84 GRR9 pKa = 11.84 SRR11 pKa = 11.84 SRR13 pKa = 11.84 SRR15 pKa = 11.84 QTQGPKK21 pKa = 10.41 GPKK24 pKa = 9.3 PEE26 pKa = 3.96 NKK28 pKa = 9.35 QIQVAHH34 pKa = 6.29 HH35 pKa = 6.51 AVDD38 pKa = 3.58 KK39 pKa = 11.41 ARR41 pKa = 11.84 GKK43 pKa = 10.04 PPGGDD48 pKa = 2.81 HH49 pKa = 6.84 GGDD52 pKa = 3.72 FVIVAHH58 pKa = 5.76 TVTVNINFNII68 pKa = 3.82
Molecular weight: 7.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.27
IPC2_protein 9.736
IPC_protein 10.482
Toseland 11.228
ProMoST 11.155
Dawson 11.257
Bjellqvist 10.994
Wikipedia 11.506
Rodwell 11.506
Grimsley 11.272
Solomon 11.506
Lehninger 11.462
Nozaki 11.199
DTASelect 10.994
Thurlkill 11.199
EMBOSS 11.652
Sillero 11.199
Patrickios 11.272
IPC_peptide 11.52
IPC2_peptide 9.853
IPC2.peptide.svr19 8.947
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1485
68
746
297.0
32.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.734 ± 1.104
1.953 ± 0.56
4.444 ± 0.547
3.502 ± 0.673
3.3 ± 0.766
6.465 ± 1.145
2.761 ± 0.761
4.916 ± 1.208
4.377 ± 0.692
9.832 ± 1.697
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.694 ± 0.451
5.185 ± 0.853
6.33 ± 0.85
3.771 ± 0.414
7.003 ± 0.577
8.754 ± 2.125
6.599 ± 1.602
7.205 ± 1.009
1.145 ± 0.244
2.963 ± 0.539
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here