Paracoccus jeotgali
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2977 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9MI93|A0A2K9MI93_9RHOB ArsR family transcriptional regulator OS=Paracoccus jeotgali OX=2065379 GN=CYR75_13645 PE=4 SV=1
MM1 pKa = 7.76 NITVLYY7 pKa = 8.67 GTEE10 pKa = 3.88 TGNAEE15 pKa = 4.04 MLAEE19 pKa = 4.46 DD20 pKa = 3.99 VAAHH24 pKa = 6.55 LEE26 pKa = 4.41 GAHH29 pKa = 6.35 EE30 pKa = 4.35 VACTNLSDD38 pKa = 4.63 FAPADD43 pKa = 3.65 FDD45 pKa = 4.07 PGQFYY50 pKa = 10.9 LVICSTYY57 pKa = 11.15 GDD59 pKa = 4.18 GEE61 pKa = 4.44 LPASAKK67 pKa = 10.11 PFAEE71 pKa = 4.04 AMEE74 pKa = 5.56 AEE76 pKa = 4.5 MPDD79 pKa = 3.26 LAGVHH84 pKa = 5.9 FAIFGLGDD92 pKa = 3.32 TEE94 pKa = 5.68 YY95 pKa = 11.01 EE96 pKa = 4.05 DD97 pKa = 3.4 THH99 pKa = 7.76 NFGSRR104 pKa = 11.84 IIAEE108 pKa = 4.08 MLQARR113 pKa = 11.84 GASQIGEE120 pKa = 4.23 RR121 pKa = 11.84 VLHH124 pKa = 6.5 DD125 pKa = 3.76 ASGGDD130 pKa = 3.6 MADD133 pKa = 3.98 DD134 pKa = 5.18 LAMDD138 pKa = 3.92 WADD141 pKa = 3.6 SVVEE145 pKa = 4.3 LAAQQMKK152 pKa = 10.51 EE153 pKa = 4.04 DD154 pKa = 3.65 AA155 pKa = 4.55
Molecular weight: 16.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.617
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.528
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.719
Sillero 3.935
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A2K9MD89|A0A2K9MD89_9RHOB L D-transpeptidase OS=Paracoccus jeotgali OX=2065379 GN=CYR75_04275 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 TRR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.63 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.41 AGRR29 pKa = 11.84 LVLNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.7 GRR40 pKa = 11.84 KK41 pKa = 8.85 RR42 pKa = 11.84 LSAA45 pKa = 3.96
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2977
0
2977
950684
41
2627
319.3
34.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.981 ± 0.068
0.809 ± 0.015
6.227 ± 0.042
5.442 ± 0.039
3.448 ± 0.031
8.851 ± 0.046
2.05 ± 0.022
5.11 ± 0.032
2.496 ± 0.036
10.369 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.732 ± 0.021
2.306 ± 0.022
5.537 ± 0.034
3.467 ± 0.025
7.524 ± 0.046
4.981 ± 0.029
5.228 ± 0.026
7.021 ± 0.033
1.417 ± 0.019
2.004 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here