Amino acid dipepetide frequency for Polaribacter porphyrae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.288AlaAla: 3.288 ± 0.079	0.427AlaCys: 0.427 ± 0.021	3.113AlaAsp: 3.113 ± 0.061	3.095AlaGlu: 3.095 ± 0.056	3.054AlaPhe: 3.054 ± 0.054	3.448AlaGly: 3.448 ± 0.064	0.869AlaHis: 0.869 ± 0.03	5.237AlaIle: 5.237 ± 0.073	4.887AlaLys: 4.887 ± 0.083	5.104AlaLeu: 5.104 ± 0.079	1.15AlaMet: 1.15 ± 0.035	3.635AlaAsn: 3.635 ± 0.072	1.518AlaPro: 1.518 ± 0.035	1.904AlaGln: 1.904 ± 0.043	1.711AlaArg: 1.711 ± 0.04	3.971AlaSer: 3.971 ± 0.066	3.564AlaThr: 3.564 ± 0.067	3.459AlaVal: 3.459 ± 0.067	0.524AlaTrp: 0.524 ± 0.022	2.187AlaTyr: 2.187 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.361CysAla: 0.361 ± 0.017	0.087CysCys: 0.087 ± 0.009	0.365CysAsp: 0.365 ± 0.018	0.377CysGlu: 0.377 ± 0.019	0.387CysPhe: 0.387 ± 0.02	0.513CysGly: 0.513 ± 0.021	0.149CysHis: 0.149 ± 0.012	0.567CysIle: 0.567 ± 0.021	0.59CysLys: 0.59 ± 0.025	0.55CysLeu: 0.55 ± 0.021	0.107CysMet: 0.107 ± 0.01	0.39CysAsn: 0.39 ± 0.018	0.233CysPro: 0.233 ± 0.016	0.169CysGln: 0.169 ± 0.013	0.154CysArg: 0.154 ± 0.013	0.496CysSer: 0.496 ± 0.021	0.369CysThr: 0.369 ± 0.019	0.369CysVal: 0.369 ± 0.018	0.058CysTrp: 0.058 ± 0.007	0.264CysTyr: 0.264 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.475AspAla: 3.475 ± 0.071	0.338AspCys: 0.338 ± 0.017	3.065AspAsp: 3.065 ± 0.074	3.705AspGlu: 3.705 ± 0.058	4.014AspPhe: 4.014 ± 0.055	3.467AspGly: 3.467 ± 0.087	0.586AspHis: 0.586 ± 0.024	4.621AspIle: 4.621 ± 0.062	4.936AspLys: 4.936 ± 0.07	5.018AspLeu: 5.018 ± 0.072	0.865AspMet: 0.865 ± 0.026	3.599AspAsn: 3.599 ± 0.063	1.288AspPro: 1.288 ± 0.039	0.996AspGln: 0.996 ± 0.029	1.58AspArg: 1.58 ± 0.04	2.972AspSer: 2.972 ± 0.064	2.699AspThr: 2.699 ± 0.065	3.571AspVal: 3.571 ± 0.06	0.7AspTrp: 0.7 ± 0.024	2.64AspTyr: 2.64 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	3.565GluAla: 3.565 ± 0.061	0.272GluCys: 0.272 ± 0.018	3.276GluAsp: 3.276 ± 0.058	4.347GluGlu: 4.347 ± 0.073	3.07GluPhe: 3.07 ± 0.05	3.117GluGly: 3.117 ± 0.057	0.935GluHis: 0.935 ± 0.029	6.103GluIle: 6.103 ± 0.078	6.541GluLys: 6.541 ± 0.1	5.632GluLeu: 5.632 ± 0.076	1.42GluMet: 1.42 ± 0.037	5.394GluAsn: 5.394 ± 0.074	1.298GluPro: 1.298 ± 0.037	1.924GluGln: 1.924 ± 0.042	2.206GluArg: 2.206 ± 0.046	3.073GluSer: 3.073 ± 0.047	3.514GluThr: 3.514 ± 0.05	3.982GluVal: 3.982 ± 0.069	0.558GluTrp: 0.558 ± 0.025	2.339GluTyr: 2.339 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.877PheAla: 2.877 ± 0.054	0.428PheCys: 0.428 ± 0.019	3.457PheAsp: 3.457 ± 0.064	3.386PheGlu: 3.386 ± 0.053	3.286PhePhe: 3.286 ± 0.081	3.744PheGly: 3.744 ± 0.067	0.788PheHis: 0.788 ± 0.022	4.67PheIle: 4.67 ± 0.079	4.546PheLys: 4.546 ± 0.078	5.463PheLeu: 5.463 ± 0.097	1.054PheMet: 1.054 ± 0.028	3.863PheAsn: 3.863 ± 0.071	1.664PhePro: 1.664 ± 0.045	1.581PheGln: 1.581 ± 0.04	1.657PheArg: 1.657 ± 0.039	4.735PheSer: 4.735 ± 0.072	3.488PheThr: 3.488 ± 0.054	3.044PheVal: 3.044 ± 0.054	0.637PheTrp: 0.637 ± 0.025	2.505PheTyr: 2.505 ± 0.055	0.0PheXaa: 0.0 ± 0.0
Gly	3.555GlyAla: 3.555 ± 0.065	0.473GlyCys: 0.473 ± 0.022	2.936GlyAsp: 2.936 ± 0.058	3.033GlyGlu: 3.033 ± 0.045	3.844GlyPhe: 3.844 ± 0.062	4.158GlyGly: 4.158 ± 0.089	0.857GlyHis: 0.857 ± 0.024	5.279GlyIle: 5.279 ± 0.067	5.184GlyLys: 5.184 ± 0.075	5.266GlyLeu: 5.266 ± 0.08	1.296GlyMet: 1.296 ± 0.035	4.038GlyAsn: 4.038 ± 0.073	0.996GlyPro: 0.996 ± 0.036	1.545GlyGln: 1.545 ± 0.038	1.901GlyArg: 1.901 ± 0.053	3.726GlySer: 3.726 ± 0.071	3.847GlyThr: 3.847 ± 0.088	3.969GlyVal: 3.969 ± 0.056	0.703GlyTrp: 0.703 ± 0.025	2.653GlyTyr: 2.653 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	0.74HisAla: 0.74 ± 0.026	0.134HisCys: 0.134 ± 0.011	0.656HisAsp: 0.656 ± 0.027	0.734HisGlu: 0.734 ± 0.029	1.106HisPhe: 1.106 ± 0.032	0.798HisGly: 0.798 ± 0.028	0.468HisHis: 0.468 ± 0.021	1.343HisIle: 1.343 ± 0.04	1.385HisLys: 1.385 ± 0.034	1.641HisLeu: 1.641 ± 0.04	0.221HisMet: 0.221 ± 0.014	0.952HisAsn: 0.952 ± 0.028	0.816HisPro: 0.816 ± 0.032	0.787HisGln: 0.787 ± 0.029	0.6HisArg: 0.6 ± 0.024	0.983HisSer: 0.983 ± 0.028	0.916HisThr: 0.916 ± 0.033	0.703HisVal: 0.703 ± 0.025	0.178HisTrp: 0.178 ± 0.013	0.721HisTyr: 0.721 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.424IleAla: 5.424 ± 0.078	0.623IleCys: 0.623 ± 0.024	5.276IleAsp: 5.276 ± 0.057	5.533IleGlu: 5.533 ± 0.074	4.343IlePhe: 4.343 ± 0.087	4.953IleGly: 4.953 ± 0.073	1.415IleHis: 1.415 ± 0.037	7.295IleIle: 7.295 ± 0.101	7.391IleLys: 7.391 ± 0.085	7.872IleLeu: 7.872 ± 0.116	1.266IleMet: 1.266 ± 0.031	5.976IleAsn: 5.976 ± 0.094	3.14IlePro: 3.14 ± 0.056	2.665IleGln: 2.665 ± 0.045	2.609IleArg: 2.609 ± 0.051	6.499IleSer: 6.499 ± 0.079	5.357IleThr: 5.357 ± 0.078	4.888IleVal: 4.888 ± 0.076	0.781IleTrp: 0.781 ± 0.026	3.269IleTyr: 3.269 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.783LysAla: 4.783 ± 0.07	0.346LysCys: 0.346 ± 0.018	4.909LysAsp: 4.909 ± 0.073	7.442LysGlu: 7.442 ± 0.099	3.572LysPhe: 3.572 ± 0.056	4.896LysGly: 4.896 ± 0.068	1.444LysHis: 1.444 ± 0.038	8.174LysIle: 8.174 ± 0.1	9.561LysLys: 9.561 ± 0.119	7.406LysLeu: 7.406 ± 0.108	2.171LysMet: 2.171 ± 0.044	7.561LysAsn: 7.561 ± 0.096	2.588LysPro: 2.588 ± 0.052	2.926LysGln: 2.926 ± 0.056	3.186LysArg: 3.186 ± 0.058	5.454LysSer: 5.454 ± 0.08	5.396LysThr: 5.396 ± 0.073	5.307LysVal: 5.307 ± 0.066	0.88LysTrp: 0.88 ± 0.029	3.645LysTyr: 3.645 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	5.081LeuAla: 5.081 ± 0.083	0.577LeuCys: 0.577 ± 0.023	4.825LeuAsp: 4.825 ± 0.06	6.202LeuGlu: 6.202 ± 0.082	5.279LeuPhe: 5.279 ± 0.093	5.541LeuGly: 5.541 ± 0.082	1.42LeuHis: 1.42 ± 0.041	7.421LeuIle: 7.421 ± 0.106	8.83LeuLys: 8.83 ± 0.107	8.582LeuLeu: 8.582 ± 0.108	1.794LeuMet: 1.794 ± 0.042	6.314LeuAsn: 6.314 ± 0.086	3.154LeuPro: 3.154 ± 0.055	3.279LeuGln: 3.279 ± 0.065	2.783LeuArg: 2.783 ± 0.056	6.532LeuSer: 6.532 ± 0.089	4.889LeuThr: 4.889 ± 0.07	4.993LeuVal: 4.993 ± 0.075	0.79LeuTrp: 0.79 ± 0.031	3.172LeuTyr: 3.172 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	1.188MetAla: 1.188 ± 0.035	0.118MetCys: 0.118 ± 0.011	0.927MetAsp: 0.927 ± 0.029	1.026MetGlu: 1.026 ± 0.033	0.778MetPhe: 0.778 ± 0.025	1.031MetGly: 1.031 ± 0.028	0.348MetHis: 0.348 ± 0.018	1.566MetIle: 1.566 ± 0.038	2.13MetLys: 2.13 ± 0.046	1.754MetLeu: 1.754 ± 0.038	0.503MetMet: 0.503 ± 0.024	1.275MetAsn: 1.275 ± 0.033	0.681MetPro: 0.681 ± 0.025	0.744MetGln: 0.744 ± 0.025	0.746MetArg: 0.746 ± 0.027	1.324MetSer: 1.324 ± 0.038	0.907MetThr: 0.907 ± 0.029	1.07MetVal: 1.07 ± 0.032	0.161MetTrp: 0.161 ± 0.013	0.684MetTyr: 0.684 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.861AsnAla: 3.861 ± 0.065	0.479AsnCys: 0.479 ± 0.024	3.621AsnAsp: 3.621 ± 0.074	3.884AsnGlu: 3.884 ± 0.059	4.021AsnPhe: 4.021 ± 0.069	4.317AsnGly: 4.317 ± 0.096	1.221AsnHis: 1.221 ± 0.036	5.964AsnIle: 5.964 ± 0.09	5.89AsnLys: 5.89 ± 0.087	6.473AsnLeu: 6.473 ± 0.089	1.188AsnMet: 1.188 ± 0.032	5.286AsnAsn: 5.286 ± 0.109	2.912AsnPro: 2.912 ± 0.055	2.754AsnGln: 2.754 ± 0.058	2.252AsnArg: 2.252 ± 0.052	4.879AsnSer: 4.879 ± 0.087	4.353AsnThr: 4.353 ± 0.077	3.695AsnVal: 3.695 ± 0.064	0.932AsnTrp: 0.932 ± 0.033	3.497AsnTyr: 3.497 ± 0.065	0.0AsnXaa: 0.0 ± 0.0
Pro	1.511ProAla: 1.511 ± 0.041	0.155ProCys: 0.155 ± 0.011	1.612ProAsp: 1.612 ± 0.039	2.185ProGlu: 2.185 ± 0.044	1.921ProPhe: 1.921 ± 0.046	1.432ProGly: 1.432 ± 0.044	0.555ProHis: 0.555 ± 0.021	2.642ProIle: 2.642 ± 0.053	2.934ProLys: 2.934 ± 0.051	2.59ProLeu: 2.59 ± 0.05	0.533ProMet: 0.533 ± 0.023	2.454ProAsn: 2.454 ± 0.046	0.646ProPro: 0.646 ± 0.027	0.892ProGln: 0.892 ± 0.028	0.849ProArg: 0.849 ± 0.024	1.913ProSer: 1.913 ± 0.046	1.933ProThr: 1.933 ± 0.048	1.811ProVal: 1.811 ± 0.043	0.311ProTrp: 0.311 ± 0.017	1.227ProTyr: 1.227 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	1.496GlnAla: 1.496 ± 0.039	0.147GlnCys: 0.147 ± 0.011	1.498GlnAsp: 1.498 ± 0.034	2.298GlnGlu: 2.298 ± 0.044	1.758GlnPhe: 1.758 ± 0.038	1.567GlnGly: 1.567 ± 0.038	0.518GlnHis: 0.518 ± 0.022	2.754GlnIle: 2.754 ± 0.053	3.411GlnLys: 3.411 ± 0.06	3.196GlnLeu: 3.196 ± 0.057	0.711GlnMet: 0.711 ± 0.026	2.471GlnAsn: 2.471 ± 0.056	0.9GlnPro: 0.9 ± 0.026	1.444GlnGln: 1.444 ± 0.041	1.083GlnArg: 1.083 ± 0.032	1.702GlnSer: 1.702 ± 0.036	1.769GlnThr: 1.769 ± 0.044	1.736GlnVal: 1.736 ± 0.042	0.268GlnTrp: 0.268 ± 0.015	1.222GlnTyr: 1.222 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	1.782ArgAla: 1.782 ± 0.044	0.161ArgCys: 0.161 ± 0.01	1.605ArgAsp: 1.605 ± 0.037	1.959ArgGlu: 1.959 ± 0.046	1.955ArgPhe: 1.955 ± 0.047	1.803ArgGly: 1.803 ± 0.044	0.49ArgHis: 0.49 ± 0.019	2.867ArgIle: 2.867 ± 0.06	3.133ArgLys: 3.133 ± 0.055	2.892ArgLeu: 2.892 ± 0.056	0.739ArgMet: 0.739 ± 0.023	2.27ArgAsn: 2.27 ± 0.047	0.887ArgPro: 0.887 ± 0.028	0.95ArgGln: 0.95 ± 0.029	1.222ArgArg: 1.222 ± 0.038	1.667ArgSer: 1.667 ± 0.045	1.791ArgThr: 1.791 ± 0.042	1.918ArgVal: 1.918 ± 0.041	0.312ArgTrp: 0.312 ± 0.015	1.399ArgTyr: 1.399 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	3.196SerAla: 3.196 ± 0.061	0.601SerCys: 0.601 ± 0.021	3.535SerAsp: 3.535 ± 0.058	3.953SerGlu: 3.953 ± 0.068	4.34SerPhe: 4.34 ± 0.068	4.35SerGly: 4.35 ± 0.074	0.986SerHis: 0.986 ± 0.033	5.945SerIle: 5.945 ± 0.074	6.087SerLys: 6.087 ± 0.081	6.231SerLeu: 6.231 ± 0.08	1.141SerMet: 1.141 ± 0.033	4.43SerAsn: 4.43 ± 0.069	1.796SerPro: 1.796 ± 0.044	1.951SerGln: 1.951 ± 0.041	1.988SerArg: 1.988 ± 0.05	4.76SerSer: 4.76 ± 0.09	3.79SerThr: 3.79 ± 0.063	3.727SerVal: 3.727 ± 0.061	0.741SerTrp: 0.741 ± 0.025	2.839SerTyr: 2.839 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	3.459ThrAla: 3.459 ± 0.073	0.346ThrCys: 0.346 ± 0.018	3.196ThrAsp: 3.196 ± 0.062	3.329ThrGlu: 3.329 ± 0.053	3.438ThrPhe: 3.438 ± 0.058	3.608ThrGly: 3.608 ± 0.071	0.982ThrHis: 0.982 ± 0.029	5.621ThrIle: 5.621 ± 0.076	4.829ThrLys: 4.829 ± 0.074	5.185ThrLeu: 5.185 ± 0.071	0.817ThrMet: 0.817 ± 0.025	4.004ThrAsn: 4.004 ± 0.083	2.217ThrPro: 2.217 ± 0.054	1.746ThrGln: 1.746 ± 0.037	1.612ThrArg: 1.612 ± 0.04	4.156ThrSer: 4.156 ± 0.069	3.692ThrThr: 3.692 ± 0.075	3.469ThrVal: 3.469 ± 0.08	0.578ThrTrp: 0.578 ± 0.025	2.402ThrTyr: 2.402 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	3.841ValAla: 3.841 ± 0.065	0.435ValCys: 0.435 ± 0.017	3.58ValAsp: 3.58 ± 0.057	3.415ValGlu: 3.415 ± 0.062	3.354ValPhe: 3.354 ± 0.055	3.522ValGly: 3.522 ± 0.058	0.861ValHis: 0.861 ± 0.029	4.716ValIle: 4.716 ± 0.068	4.69ValLys: 4.69 ± 0.056	5.722ValLeu: 5.722 ± 0.083	1.06ValMet: 1.06 ± 0.031	3.803ValAsn: 3.803 ± 0.068	1.757ValPro: 1.757 ± 0.038	1.554ValGln: 1.554 ± 0.035	1.772ValArg: 1.772 ± 0.038	4.129ValSer: 4.129 ± 0.056	3.369ValThr: 3.369 ± 0.075	3.68ValVal: 3.68 ± 0.066	0.579ValTrp: 0.579 ± 0.022	2.218ValTyr: 2.218 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.521TrpAla: 0.521 ± 0.02	0.094TrpCys: 0.094 ± 0.009	0.524TrpAsp: 0.524 ± 0.017	0.61TrpGlu: 0.61 ± 0.024	0.642TrpPhe: 0.642 ± 0.027	0.628TrpGly: 0.628 ± 0.026	0.206TrpHis: 0.206 ± 0.013	0.795TrpIle: 0.795 ± 0.027	0.921TrpLys: 0.921 ± 0.028	0.957TrpLeu: 0.957 ± 0.027	0.284TrpMet: 0.284 ± 0.015	0.765TrpAsn: 0.765 ± 0.029	0.165TrpPro: 0.165 ± 0.013	0.421TrpGln: 0.421 ± 0.022	0.384TrpArg: 0.384 ± 0.017	0.654TrpSer: 0.654 ± 0.029	0.534TrpThr: 0.534 ± 0.026	0.589TrpVal: 0.589 ± 0.024	0.157TrpTrp: 0.157 ± 0.013	0.446TrpTyr: 0.446 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.145TyrAla: 2.145 ± 0.044	0.315TyrCys: 0.315 ± 0.016	2.163TyrAsp: 2.163 ± 0.055	2.055TyrGlu: 2.055 ± 0.04	2.711TyrPhe: 2.711 ± 0.054	2.365TyrGly: 2.365 ± 0.046	0.782TyrHis: 0.782 ± 0.026	2.995TyrIle: 2.995 ± 0.06	3.771TyrLys: 3.771 ± 0.064	3.924TyrLeu: 3.924 ± 0.064	0.639TyrMet: 0.639 ± 0.021	3.0TyrAsn: 3.0 ± 0.06	1.449TyrPro: 1.449 ± 0.038	1.717TyrGln: 1.717 ± 0.037	1.507TyrArg: 1.507 ± 0.034	2.762TyrSer: 2.762 ± 0.052	2.507TyrThr: 2.507 ± 0.062	2.076TyrVal: 2.076 ± 0.05	0.449TyrTrp: 0.449 ± 0.019	1.846TyrTyr: 1.846 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3404 proteins (1172143 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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