Sophora alopecuroides yellow stunt alphasatellite 8
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5YSH3|A0A1W5YSH3_9VIRU Satellite replication initiator protein OS=Sophora alopecuroides yellow stunt alphasatellite 8 OX=1980173 PE=4 SV=1
MM1 pKa = 7.98 PSQKK5 pKa = 8.99 STCWVFTLNFEE16 pKa = 4.51 GDD18 pKa = 3.83 PPTLTFDD25 pKa = 5.33 DD26 pKa = 4.08 RR27 pKa = 11.84 VQYY30 pKa = 11.05 ASWQHH35 pKa = 5.1 EE36 pKa = 4.64 RR37 pKa = 11.84 VGHH40 pKa = 5.97 DD41 pKa = 3.24 HH42 pKa = 6.69 LQGFIQFKK50 pKa = 10.55 SRR52 pKa = 11.84 NTTMGQCKK60 pKa = 9.83 AQFNGLSPHH69 pKa = 6.95 LEE71 pKa = 3.61 IARR74 pKa = 11.84 DD75 pKa = 3.61 VNKK78 pKa = 10.54 AQEE81 pKa = 4.13 YY82 pKa = 8.8 TMKK85 pKa = 10.39 EE86 pKa = 4.06 DD87 pKa = 3.49 TRR89 pKa = 11.84 IAGPWEE95 pKa = 3.9 YY96 pKa = 11.4 GVMIKK101 pKa = 10.33 KK102 pKa = 10.28 GSHH105 pKa = 4.91 KK106 pKa = 10.1 RR107 pKa = 11.84 KK108 pKa = 9.94 LMEE111 pKa = 4.6 KK112 pKa = 10.26 FEE114 pKa = 4.53 EE115 pKa = 4.82 DD116 pKa = 3.56 PEE118 pKa = 4.16 EE119 pKa = 4.08 MKK121 pKa = 10.7 IADD124 pKa = 3.53 PSLYY128 pKa = 10.0 RR129 pKa = 11.84 RR130 pKa = 11.84 CLTRR134 pKa = 11.84 KK135 pKa = 8.7 MSEE138 pKa = 4.1 EE139 pKa = 3.94 QRR141 pKa = 11.84 SSAEE145 pKa = 3.47 WNYY148 pKa = 11.24 DD149 pKa = 3.23 LRR151 pKa = 11.84 PWQEE155 pKa = 3.37 AVIQEE160 pKa = 4.5 LEE162 pKa = 4.23 LPPDD166 pKa = 3.34 YY167 pKa = 10.99 RR168 pKa = 11.84 KK169 pKa = 10.23 IIWVYY174 pKa = 10.71 GPGGNEE180 pKa = 4.03 GKK182 pKa = 8.49 STFARR187 pKa = 11.84 HH188 pKa = 6.0 LSLKK192 pKa = 9.77 EE193 pKa = 3.4 GWGYY197 pKa = 10.96 VPGGRR202 pKa = 11.84 TQDD205 pKa = 3.09 MMHH208 pKa = 7.46 IITADD213 pKa = 3.75 PKK215 pKa = 8.93 NHH217 pKa = 5.83 WVFDD221 pKa = 3.86 VPRR224 pKa = 11.84 VSSEE228 pKa = 3.72 YY229 pKa = 10.22 VNYY232 pKa = 10.52 GVIEE236 pKa = 4.05 QVKK239 pKa = 9.26 NRR241 pKa = 11.84 VMVNTKK247 pKa = 10.11 YY248 pKa = 10.08 EE249 pKa = 4.13 PCVMRR254 pKa = 11.84 DD255 pKa = 3.5 DD256 pKa = 3.89 NHH258 pKa = 6.28 PVHH261 pKa = 6.57 VVVFANCLPDD271 pKa = 3.58 VTKK274 pKa = 10.94 LSEE277 pKa = 4.03 DD278 pKa = 3.48 RR279 pKa = 11.84 IKK281 pKa = 10.49 IIKK284 pKa = 9.41 CC285 pKa = 3.38
Molecular weight: 33.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.364
IPC2_protein 6.338
IPC_protein 6.478
Toseland 6.664
ProMoST 6.912
Dawson 6.868
Bjellqvist 6.795
Wikipedia 6.868
Rodwell 6.868
Grimsley 6.781
Solomon 6.883
Lehninger 6.883
Nozaki 7.117
DTASelect 7.263
Thurlkill 7.293
EMBOSS 7.293
Sillero 7.263
Patrickios 4.304
IPC_peptide 6.898
IPC2_peptide 6.956
IPC2.peptide.svr19 6.865
Protein with the highest isoelectric point:
>tr|A0A1W5YSH3|A0A1W5YSH3_9VIRU Satellite replication initiator protein OS=Sophora alopecuroides yellow stunt alphasatellite 8 OX=1980173 PE=4 SV=1
MM1 pKa = 7.98 PSQKK5 pKa = 8.99 STCWVFTLNFEE16 pKa = 4.51 GDD18 pKa = 3.83 PPTLTFDD25 pKa = 5.33 DD26 pKa = 4.08 RR27 pKa = 11.84 VQYY30 pKa = 11.05 ASWQHH35 pKa = 5.1 EE36 pKa = 4.64 RR37 pKa = 11.84 VGHH40 pKa = 5.97 DD41 pKa = 3.24 HH42 pKa = 6.69 LQGFIQFKK50 pKa = 10.55 SRR52 pKa = 11.84 NTTMGQCKK60 pKa = 9.83 AQFNGLSPHH69 pKa = 6.95 LEE71 pKa = 3.61 IARR74 pKa = 11.84 DD75 pKa = 3.61 VNKK78 pKa = 10.54 AQEE81 pKa = 4.13 YY82 pKa = 8.8 TMKK85 pKa = 10.39 EE86 pKa = 4.06 DD87 pKa = 3.49 TRR89 pKa = 11.84 IAGPWEE95 pKa = 3.9 YY96 pKa = 11.4 GVMIKK101 pKa = 10.33 KK102 pKa = 10.28 GSHH105 pKa = 4.91 KK106 pKa = 10.1 RR107 pKa = 11.84 KK108 pKa = 9.94 LMEE111 pKa = 4.6 KK112 pKa = 10.26 FEE114 pKa = 4.53 EE115 pKa = 4.82 DD116 pKa = 3.56 PEE118 pKa = 4.16 EE119 pKa = 4.08 MKK121 pKa = 10.7 IADD124 pKa = 3.53 PSLYY128 pKa = 10.0 RR129 pKa = 11.84 RR130 pKa = 11.84 CLTRR134 pKa = 11.84 KK135 pKa = 8.7 MSEE138 pKa = 4.1 EE139 pKa = 3.94 QRR141 pKa = 11.84 SSAEE145 pKa = 3.47 WNYY148 pKa = 11.24 DD149 pKa = 3.23 LRR151 pKa = 11.84 PWQEE155 pKa = 3.37 AVIQEE160 pKa = 4.5 LEE162 pKa = 4.23 LPPDD166 pKa = 3.34 YY167 pKa = 10.99 RR168 pKa = 11.84 KK169 pKa = 10.23 IIWVYY174 pKa = 10.71 GPGGNEE180 pKa = 4.03 GKK182 pKa = 8.49 STFARR187 pKa = 11.84 HH188 pKa = 6.0 LSLKK192 pKa = 9.77 EE193 pKa = 3.4 GWGYY197 pKa = 10.96 VPGGRR202 pKa = 11.84 TQDD205 pKa = 3.09 MMHH208 pKa = 7.46 IITADD213 pKa = 3.75 PKK215 pKa = 8.93 NHH217 pKa = 5.83 WVFDD221 pKa = 3.86 VPRR224 pKa = 11.84 VSSEE228 pKa = 3.72 YY229 pKa = 10.22 VNYY232 pKa = 10.52 GVIEE236 pKa = 4.05 QVKK239 pKa = 9.26 NRR241 pKa = 11.84 VMVNTKK247 pKa = 10.11 YY248 pKa = 10.08 EE249 pKa = 4.13 PCVMRR254 pKa = 11.84 DD255 pKa = 3.5 DD256 pKa = 3.89 NHH258 pKa = 6.28 PVHH261 pKa = 6.57 VVVFANCLPDD271 pKa = 3.58 VTKK274 pKa = 10.94 LSEE277 pKa = 4.03 DD278 pKa = 3.48 RR279 pKa = 11.84 IKK281 pKa = 10.49 IIKK284 pKa = 9.41 CC285 pKa = 3.38
Molecular weight: 33.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.364
IPC2_protein 6.338
IPC_protein 6.478
Toseland 6.664
ProMoST 6.912
Dawson 6.868
Bjellqvist 6.795
Wikipedia 6.868
Rodwell 6.868
Grimsley 6.781
Solomon 6.883
Lehninger 6.883
Nozaki 7.117
DTASelect 7.263
Thurlkill 7.293
EMBOSS 7.293
Sillero 7.263
Patrickios 4.304
IPC_peptide 6.898
IPC2_peptide 6.956
IPC2.peptide.svr19 6.865
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
285
285
285
285.0
33.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.86 ± 0.0
2.105 ± 0.0
5.965 ± 0.0
8.07 ± 0.0
3.509 ± 0.0
5.965 ± 0.0
3.509 ± 0.0
4.912 ± 0.0
7.368 ± 0.0
5.263 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.86 ± 0.0
4.211 ± 0.0
5.965 ± 0.0
4.561 ± 0.0
6.316 ± 0.0
5.263 ± 0.0
4.912 ± 0.0
7.719 ± 0.0
2.807 ± 0.0
3.86 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here