Amino acid dipepetide frequency for Mycoplasma hyosynoviae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.693AlaAla: 2.693 ± 0.109
0.28AlaCys: 0.28 ± 0.032
2.146AlaAsp: 2.146 ± 0.101
2.956AlaGlu: 2.956 ± 0.116
2.496AlaPhe: 2.496 ± 0.118
2.296AlaGly: 2.296 ± 0.125
0.655AlaHis: 0.655 ± 0.048
5.231AlaIle: 5.231 ± 0.161
5.915AlaLys: 5.915 ± 0.211
4.609AlaLeu: 4.609 ± 0.162
0.96AlaMet: 0.96 ± 0.063
3.49AlaAsn: 3.49 ± 0.129
1.081AlaPro: 1.081 ± 0.078
1.607AlaGln: 1.607 ± 0.074
1.44AlaArg: 1.44 ± 0.106
3.527AlaSer: 3.527 ± 0.128
2.993AlaThr: 2.993 ± 0.117
1.97AlaVal: 1.97 ± 0.093
0.321AlaTrp: 0.321 ± 0.04
1.966AlaTyr: 1.966 ± 0.093
0.0AlaXaa: 0.0 ± 0.0
Cys
0.271CysAla: 0.271 ± 0.032
0.063CysCys: 0.063 ± 0.017
0.309CysAsp: 0.309 ± 0.039
0.493CysGlu: 0.493 ± 0.048
0.217CysPhe: 0.217 ± 0.027
0.409CysGly: 0.409 ± 0.046
0.083CysHis: 0.083 ± 0.021
0.463CysIle: 0.463 ± 0.052
0.584CysLys: 0.584 ± 0.056
0.493CysLeu: 0.493 ± 0.046
0.05CysMet: 0.05 ± 0.013
0.363CysAsn: 0.363 ± 0.038
0.209CysPro: 0.209 ± 0.031
0.129CysGln: 0.129 ± 0.02
0.146CysArg: 0.146 ± 0.025
0.397CysSer: 0.397 ± 0.047
0.196CysThr: 0.196 ± 0.03
0.317CysVal: 0.317 ± 0.038
0.042CysTrp: 0.042 ± 0.012
0.259CysTyr: 0.259 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
2.914AspAla: 2.914 ± 0.103
0.225AspCys: 0.225 ± 0.03
3.093AspAsp: 3.093 ± 0.134
4.55AspGlu: 4.55 ± 0.157
3.799AspPhe: 3.799 ± 0.149
2.538AspGly: 2.538 ± 0.112
0.693AspHis: 0.693 ± 0.053
5.151AspIle: 5.151 ± 0.144
6.913AspLys: 6.913 ± 0.209
5.293AspLeu: 5.293 ± 0.158
0.676AspMet: 0.676 ± 0.048
3.502AspAsn: 3.502 ± 0.131
1.716AspPro: 1.716 ± 0.09
1.252AspGln: 1.252 ± 0.072
1.24AspArg: 1.24 ± 0.07
3.122AspSer: 3.122 ± 0.115
2.196AspThr: 2.196 ± 0.088
3.227AspVal: 3.227 ± 0.123
0.526AspTrp: 0.526 ± 0.044
2.684AspTyr: 2.684 ± 0.115
0.0AspXaa: 0.0 ± 0.0
Glu
3.315GluAla: 3.315 ± 0.134
0.338GluCys: 0.338 ± 0.038
3.636GluAsp: 3.636 ± 0.145
5.857GluGlu: 5.857 ± 0.276
4.028GluPhe: 4.028 ± 0.145
2.617GluGly: 2.617 ± 0.107
1.035GluHis: 1.035 ± 0.064
8.654GluIle: 8.654 ± 0.181
8.904GluLys: 8.904 ± 0.197
6.667GluLeu: 6.667 ± 0.178
1.261GluMet: 1.261 ± 0.067
6.349GluAsn: 6.349 ± 0.205
1.302GluPro: 1.302 ± 0.081
2.789GluGln: 2.789 ± 0.149
1.636GluArg: 1.636 ± 0.091
3.294GluSer: 3.294 ± 0.148
3.916GluThr: 3.916 ± 0.128
4.091GluVal: 4.091 ± 0.143
0.68GluTrp: 0.68 ± 0.05
3.323GluTyr: 3.323 ± 0.135
0.0GluXaa: 0.0 ± 0.0
Phe
3.047PheAla: 3.047 ± 0.108
0.296PheCys: 0.296 ± 0.037
3.886PheAsp: 3.886 ± 0.13
4.329PheGlu: 4.329 ± 0.137
2.563PhePhe: 2.563 ± 0.108
2.371PheGly: 2.371 ± 0.102
0.576PheHis: 0.576 ± 0.052
5.059PheIle: 5.059 ± 0.213
5.769PheLys: 5.769 ± 0.177
5.11PheLeu: 5.11 ± 0.204
0.785PheMet: 0.785 ± 0.061
4.024PheAsn: 4.024 ± 0.143
1.206PhePro: 1.206 ± 0.071
1.457PheGln: 1.457 ± 0.08
1.54PheArg: 1.54 ± 0.083
4.354PheSer: 4.354 ± 0.142
2.943PheThr: 2.943 ± 0.126
2.951PheVal: 2.951 ± 0.101
0.584PheTrp: 0.584 ± 0.053
2.425PheTyr: 2.425 ± 0.121
0.0PheXaa: 0.0 ± 0.0
Gly
2.141GlyAla: 2.141 ± 0.106
0.209GlyCys: 0.209 ± 0.029
2.087GlyAsp: 2.087 ± 0.099
2.688GlyGlu: 2.688 ± 0.087
2.772GlyPhe: 2.772 ± 0.11
2.517GlyGly: 2.517 ± 0.141
0.81GlyHis: 0.81 ± 0.058
4.567GlyIle: 4.567 ± 0.16
5.16GlyLys: 5.16 ± 0.157
3.552GlyLeu: 3.552 ± 0.116
0.86GlyMet: 0.86 ± 0.057
2.342GlyAsn: 2.342 ± 0.103
0.801GlyPro: 0.801 ± 0.069
1.398GlyGln: 1.398 ± 0.087
1.394GlyArg: 1.394 ± 0.098
3.072GlySer: 3.072 ± 0.129
2.446GlyThr: 2.446 ± 0.11
2.4GlyVal: 2.4 ± 0.106
0.442GlyTrp: 0.442 ± 0.042
1.845GlyTyr: 1.845 ± 0.097
0.0GlyXaa: 0.0 ± 0.0
His
0.63HisAla: 0.63 ± 0.055
0.117HisCys: 0.117 ± 0.024
0.501HisAsp: 0.501 ± 0.047
0.63HisGlu: 0.63 ± 0.057
0.948HisPhe: 0.948 ± 0.063
0.726HisGly: 0.726 ± 0.061
0.301HisHis: 0.301 ± 0.045
1.223HisIle: 1.223 ± 0.069
1.453HisLys: 1.453 ± 0.072
1.294HisLeu: 1.294 ± 0.077
0.246HisMet: 0.246 ± 0.034
1.106HisAsn: 1.106 ± 0.083
0.605HisPro: 0.605 ± 0.052
0.388HisGln: 0.388 ± 0.04
0.346HisArg: 0.346 ± 0.035
0.964HisSer: 0.964 ± 0.062
0.672HisThr: 0.672 ± 0.05
0.426HisVal: 0.426 ± 0.05
0.121HisTrp: 0.121 ± 0.028
0.68HisTyr: 0.68 ± 0.056
0.0HisXaa: 0.0 ± 0.0
Ile
5.18IleAla: 5.18 ± 0.168
0.655IleCys: 0.655 ± 0.057
6.086IleAsp: 6.086 ± 0.15
7.401IleGlu: 7.401 ± 0.208
5.272IlePhe: 5.272 ± 0.214
4.191IleGly: 4.191 ± 0.163
1.16IleHis: 1.16 ± 0.086
8.491IleIle: 8.491 ± 0.222
11.526IleLys: 11.526 ± 0.255
8.224IleLeu: 8.224 ± 0.231
1.361IleMet: 1.361 ± 0.076
7.276IleAsn: 7.276 ± 0.198
2.826IlePro: 2.826 ± 0.124
2.834IleGln: 2.834 ± 0.106
2.375IleArg: 2.375 ± 0.114
7.41IleSer: 7.41 ± 0.191
5.807IleThr: 5.807 ± 0.173
5.343IleVal: 5.343 ± 0.158
0.731IleTrp: 0.731 ± 0.049
3.807IleTyr: 3.807 ± 0.135
0.0IleXaa: 0.0 ± 0.0
Lys
5.051LysAla: 5.051 ± 0.186
0.451LysCys: 0.451 ± 0.047
6.566LysAsp: 6.566 ± 0.216
8.424LysGlu: 8.424 ± 0.179
5.736LysPhe: 5.736 ± 0.185
3.636LysGly: 3.636 ± 0.125
1.403LysHis: 1.403 ± 0.065
12.352LysIle: 12.352 ± 0.222
11.542LysLys: 11.542 ± 0.267
10.925LysLeu: 10.925 ± 0.22
2.484LysMet: 2.484 ± 0.119
8.988LysAsn: 8.988 ± 0.186
3.043LysPro: 3.043 ± 0.123
4.078LysGln: 4.078 ± 0.128
2.805LysArg: 2.805 ± 0.107
6.391LysSer: 6.391 ± 0.182
6.642LysThr: 6.642 ± 0.188
6.103LysVal: 6.103 ± 0.238
1.11LysTrp: 1.11 ± 0.066
5.368LysTyr: 5.368 ± 0.19
0.0LysXaa: 0.0 ± 0.0
Leu
4.909LeuAla: 4.909 ± 0.136
0.522LeuCys: 0.522 ± 0.045
5.598LeuAsp: 5.598 ± 0.15
7.067LeuGlu: 7.067 ± 0.215
4.667LeuPhe: 4.667 ± 0.162
4.191LeuGly: 4.191 ± 0.114
1.19LeuHis: 1.19 ± 0.068
8.595LeuIle: 8.595 ± 0.244
10.54LeuLys: 10.54 ± 0.24
8.491LeuLeu: 8.491 ± 0.285
1.478LeuMet: 1.478 ± 0.077
7.46LeuAsn: 7.46 ± 0.171
2.88LeuPro: 2.88 ± 0.123
2.814LeuGln: 2.814 ± 0.113
2.496LeuArg: 2.496 ± 0.112
6.475LeuSer: 6.475 ± 0.163
5.118LeuThr: 5.118 ± 0.15
4.709LeuVal: 4.709 ± 0.156
0.71LeuTrp: 0.71 ± 0.057
3.198LeuTyr: 3.198 ± 0.116
0.0LeuXaa: 0.0 ± 0.0
Met
0.918MetAla: 0.918 ± 0.064
0.067MetCys: 0.067 ± 0.016
0.718MetAsp: 0.718 ± 0.057
0.835MetGlu: 0.835 ± 0.065
0.956MetPhe: 0.956 ± 0.068
0.651MetGly: 0.651 ± 0.061
0.221MetHis: 0.221 ± 0.031
1.298MetIle: 1.298 ± 0.083
1.824MetLys: 1.824 ± 0.088
1.599MetLeu: 1.599 ± 0.076
0.309MetMet: 0.309 ± 0.038
1.181MetAsn: 1.181 ± 0.072
0.751MetPro: 0.751 ± 0.048
0.768MetGln: 0.768 ± 0.066
0.468MetArg: 0.468 ± 0.051
1.131MetSer: 1.131 ± 0.07
0.814MetThr: 0.814 ± 0.053
0.793MetVal: 0.793 ± 0.064
0.142MetTrp: 0.142 ± 0.022
0.543MetTyr: 0.543 ± 0.049
0.0MetXaa: 0.0 ± 0.0
Asn
3.365AsnAla: 3.365 ± 0.124
0.296AsnCys: 0.296 ± 0.037
4.07AsnAsp: 4.07 ± 0.136
5.635AsnGlu: 5.635 ± 0.2
4.379AsnPhe: 4.379 ± 0.138
3.315AsnGly: 3.315 ± 0.122
0.86AsnHis: 0.86 ± 0.055
7.347AsnIle: 7.347 ± 0.18
8.332AsnLys: 8.332 ± 0.18
7.41AsnLeu: 7.41 ± 0.206
1.044AsnMet: 1.044 ± 0.069
6.078AsnAsn: 6.078 ± 0.217
2.425AsnPro: 2.425 ± 0.098
2.588AsnGln: 2.588 ± 0.105
1.607AsnArg: 1.607 ± 0.08
4.792AsnSer: 4.792 ± 0.137
3.515AsnThr: 3.515 ± 0.12
3.849AsnVal: 3.849 ± 0.148
0.68AsnTrp: 0.68 ± 0.055
3.77AsnTyr: 3.77 ± 0.115
0.0AsnXaa: 0.0 ± 0.0
Pro
1.29ProAla: 1.29 ± 0.077
0.1ProCys: 0.1 ± 0.022
1.336ProAsp: 1.336 ± 0.08
2.162ProGlu: 2.162 ± 0.092
1.415ProPhe: 1.415 ± 0.081
1.353ProGly: 1.353 ± 0.09
0.488ProHis: 0.488 ± 0.041
2.409ProIle: 2.409 ± 0.111
2.839ProLys: 2.839 ± 0.11
2.455ProLeu: 2.455 ± 0.101
0.355ProMet: 0.355 ± 0.038
2.154ProAsn: 2.154 ± 0.101
0.601ProPro: 0.601 ± 0.056
1.123ProGln: 1.123 ± 0.077
0.559ProArg: 0.559 ± 0.044
2.045ProSer: 2.045 ± 0.087
1.666ProThr: 1.666 ± 0.094
1.307ProVal: 1.307 ± 0.071
0.246ProTrp: 0.246 ± 0.038
1.156ProTyr: 1.156 ± 0.067
0.0ProXaa: 0.0 ± 0.0
Gln
1.536GlnAla: 1.536 ± 0.075
0.104GlnCys: 0.104 ± 0.021
1.712GlnAsp: 1.712 ± 0.086
2.718GlnGlu: 2.718 ± 0.142
1.657GlnPhe: 1.657 ± 0.081
1.169GlnGly: 1.169 ± 0.074
0.468GlnHis: 0.468 ± 0.046
3.269GlnIle: 3.269 ± 0.122
4.095GlnLys: 4.095 ± 0.14
2.734GlnLeu: 2.734 ± 0.113
0.555GlnMet: 0.555 ± 0.042
2.814GlnAsn: 2.814 ± 0.11
0.718GlnPro: 0.718 ± 0.048
1.19GlnGln: 1.19 ± 0.088
0.864GlnArg: 0.864 ± 0.067
1.774GlnSer: 1.774 ± 0.086
2.125GlnThr: 2.125 ± 0.1
1.582GlnVal: 1.582 ± 0.081
0.242GlnTrp: 0.242 ± 0.032
1.34GlnTyr: 1.34 ± 0.075
0.0GlnXaa: 0.0 ± 0.0
Arg
1.119ArgAla: 1.119 ± 0.066
0.175ArgCys: 0.175 ± 0.027
1.39ArgAsp: 1.39 ± 0.082
1.966ArgGlu: 1.966 ± 0.093
1.248ArgPhe: 1.248 ± 0.066
1.186ArgGly: 1.186 ± 0.104
0.401ArgHis: 0.401 ± 0.045
2.526ArgIle: 2.526 ± 0.109
2.905ArgLys: 2.905 ± 0.108
2.242ArgLeu: 2.242 ± 0.095
0.493ArgMet: 0.493 ± 0.051
2.029ArgAsn: 2.029 ± 0.097
0.676ArgPro: 0.676 ± 0.058
0.952ArgGln: 0.952 ± 0.057
0.898ArgArg: 0.898 ± 0.071
1.344ArgSer: 1.344 ± 0.076
1.403ArgThr: 1.403 ± 0.072
1.344ArgVal: 1.344 ± 0.091
0.238ArgTrp: 0.238 ± 0.036
1.144ArgTyr: 1.144 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
2.814SerAla: 2.814 ± 0.128
0.493SerCys: 0.493 ± 0.048
3.532SerAsp: 3.532 ± 0.124
4.667SerGlu: 4.667 ± 0.197
4.216SerPhe: 4.216 ± 0.149
3.181SerGly: 3.181 ± 0.112
0.968SerHis: 0.968 ± 0.06
6.374SerIle: 6.374 ± 0.153
7.109SerLys: 7.109 ± 0.18
6.337SerLeu: 6.337 ± 0.179
0.927SerMet: 0.927 ± 0.068
5.055SerAsn: 5.055 ± 0.165
1.595SerPro: 1.595 ± 0.084
2.438SerGln: 2.438 ± 0.1
1.682SerArg: 1.682 ± 0.086
5.009SerSer: 5.009 ± 0.182
3.836SerThr: 3.836 ± 0.154
3.06SerVal: 3.06 ± 0.123
0.497SerTrp: 0.497 ± 0.045
2.797SerTyr: 2.797 ± 0.117
0.0SerXaa: 0.0 ± 0.0
Thr
2.329ThrAla: 2.329 ± 0.092
0.271ThrCys: 0.271 ± 0.033
2.342ThrAsp: 2.342 ± 0.107
3.331ThrGlu: 3.331 ± 0.111
3.11ThrPhe: 3.11 ± 0.116
2.413ThrGly: 2.413 ± 0.12
0.81ThrHis: 0.81 ± 0.059
5.61ThrIle: 5.61 ± 0.147
6.429ThrLys: 6.429 ± 0.21
5.544ThrLeu: 5.544 ± 0.159
0.722ThrMet: 0.722 ± 0.057
3.982ThrAsn: 3.982 ± 0.135
1.995ThrPro: 1.995 ± 0.11
1.732ThrGln: 1.732 ± 0.077
1.344ThrArg: 1.344 ± 0.074
3.92ThrSer: 3.92 ± 0.13
2.918ThrThr: 2.918 ± 0.115
2.563ThrVal: 2.563 ± 0.108
0.451ThrTrp: 0.451 ± 0.047
2.137ThrTyr: 2.137 ± 0.102
0.0ThrXaa: 0.0 ± 0.0
Val
2.659ValAla: 2.659 ± 0.125
0.472ValCys: 0.472 ± 0.046
3.081ValAsp: 3.081 ± 0.126
4.016ValGlu: 4.016 ± 0.144
2.597ValPhe: 2.597 ± 0.118
2.409ValGly: 2.409 ± 0.124
0.643ValHis: 0.643 ± 0.051
4.784ValIle: 4.784 ± 0.146
5.619ValLys: 5.619 ± 0.168
4.884ValLeu: 4.884 ± 0.11
0.756ValMet: 0.756 ± 0.056
3.573ValAsn: 3.573 ± 0.126
1.398ValPro: 1.398 ± 0.077
1.49ValGln: 1.49 ± 0.081
1.298ValArg: 1.298 ± 0.078
4.053ValSer: 4.053 ± 0.133
2.292ValThr: 2.292 ± 0.122
2.939ValVal: 2.939 ± 0.138
0.405ValTrp: 0.405 ± 0.05
2.062ValTyr: 2.062 ± 0.081
0.0ValXaa: 0.0 ± 0.0
Trp
0.397TrpAla: 0.397 ± 0.045
0.029TrpCys: 0.029 ± 0.012
0.572TrpAsp: 0.572 ± 0.042
0.493TrpGlu: 0.493 ± 0.052
0.451TrpPhe: 0.451 ± 0.043
0.334TrpGly: 0.334 ± 0.036
0.117TrpHis: 0.117 ± 0.023
0.877TrpIle: 0.877 ± 0.055
1.115TrpLys: 1.115 ± 0.065
0.756TrpLeu: 0.756 ± 0.051
0.196TrpMet: 0.196 ± 0.031
0.793TrpAsn: 0.793 ± 0.067
0.188TrpPro: 0.188 ± 0.029
0.205TrpGln: 0.205 ± 0.03
0.242TrpArg: 0.242 ± 0.037
0.559TrpSer: 0.559 ± 0.048
0.505TrpThr: 0.505 ± 0.042
0.493TrpVal: 0.493 ± 0.045
0.088TrpTrp: 0.088 ± 0.017
0.376TrpTyr: 0.376 ± 0.042
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.054TyrAla: 2.054 ± 0.092
0.33TyrCys: 0.33 ± 0.032
2.651TyrAsp: 2.651 ± 0.094
3.473TyrGlu: 3.473 ± 0.139
2.751TyrPhe: 2.751 ± 0.119
2.004TyrGly: 2.004 ± 0.094
0.497TyrHis: 0.497 ± 0.048
3.678TyrIle: 3.678 ± 0.128
4.525TyrLys: 4.525 ± 0.136
4.492TyrLeu: 4.492 ± 0.134
0.468TyrMet: 0.468 ± 0.045
2.801TyrAsn: 2.801 ± 0.122
1.039TyrPro: 1.039 ± 0.074
1.432TyrGln: 1.432 ± 0.068
1.294TyrArg: 1.294 ± 0.072
2.951TyrSer: 2.951 ± 0.12
1.954TyrThr: 1.954 ± 0.096
1.962TyrVal: 1.962 ± 0.096
0.526TyrTrp: 0.526 ± 0.049
2.167TyrTyr: 2.167 ± 0.113
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 584 proteins (239554 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski