Vibrio phage VCY-phi

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Vicialiavirus; Vibrio virus VCY

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8IRU5|G8IRU5_9VIRU DNA replication initiation protein OS=Vibrio phage VCY-phi OX=1105327 PE=4 SV=1
MM1 pKa = 7.64ANMVIRR7 pKa = 11.84FGDD10 pKa = 3.89SHH12 pKa = 6.08WTTVMIKK19 pKa = 10.28NSPVYY24 pKa = 9.91YY25 pKa = 8.81WQEE28 pKa = 3.42SDD30 pKa = 3.9GYY32 pKa = 8.25YY33 pKa = 10.15VCIDD37 pKa = 3.33GRR39 pKa = 11.84PDD41 pKa = 3.66FFNTSTEE48 pKa = 4.02MYY50 pKa = 10.22LFAASEE56 pKa = 4.3GRR58 pKa = 11.84DD59 pKa = 3.58VIQVTDD65 pKa = 4.1EE66 pKa = 3.74NDD68 pKa = 2.99QALRR72 pKa = 11.84EE73 pKa = 4.21SGAFNGQGDD82 pKa = 3.84FF83 pKa = 4.13

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8IRU9|G8IRU9_9VIRU Minor capsid protein OS=Vibrio phage VCY-phi OX=1105327 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 9.97FRR4 pKa = 11.84NMAKK8 pKa = 10.12KK9 pKa = 10.25FGVVVAASVPSYY21 pKa = 11.33GVFAADD27 pKa = 5.46DD28 pKa = 3.43ISAQLTAAITSGQANYY44 pKa = 9.9TMVVVGVIGLAAIAFGLGRR63 pKa = 11.84ILGILKK69 pKa = 10.39

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

2126

61

475

193.3

21.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.914 ± 0.699

1.552 ± 0.16

5.597 ± 0.377

5.221 ± 0.387

5.833 ± 0.615

7.479 ± 0.723

1.176 ± 0.265

6.491 ± 0.411

5.644 ± 0.68

9.784 ± 0.712

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.422

5.833 ± 0.766

3.716 ± 0.466

4.233 ± 0.211

4.139 ± 0.635

7.432 ± 0.997

5.456 ± 0.619

5.833 ± 0.407

1.129 ± 0.274

4.045 ± 0.708
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski