Lactococcus phage phiL47

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Audreyjarvisvirus; Lactococcus virus phiL47

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9VEQ4|V9VEQ4_9CAUD Uncharacterized protein OS=Lactococcus phage phiL47 OX=1412875 GN=T548_0035 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.41VYY4 pKa = 10.92VLTADD9 pKa = 3.4TYY11 pKa = 10.57EE12 pKa = 4.4SYY14 pKa = 11.05GAQIEE19 pKa = 4.39LFGVFTTEE27 pKa = 3.39EE28 pKa = 4.2KK29 pKa = 10.64AEE31 pKa = 4.02KK32 pKa = 9.83RR33 pKa = 11.84ASEE36 pKa = 4.18MKK38 pKa = 10.28LDD40 pKa = 4.25CPDD43 pKa = 3.26ISVVNVDD50 pKa = 3.55EE51 pKa = 5.33NDD53 pKa = 3.37VQGYY57 pKa = 9.23LGGYY61 pKa = 9.2IEE63 pKa = 4.53

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9VHX0|V9VHX0_9CAUD Uncharacterized protein OS=Lactococcus phage phiL47 OX=1412875 GN=T548_0064 PE=4 SV=1
MM1 pKa = 7.08FKK3 pKa = 10.67KK4 pKa = 10.48RR5 pKa = 11.84ITRR8 pKa = 11.84LITVIVSYY16 pKa = 11.05LLLIGTYY23 pKa = 9.12WIYY26 pKa = 8.9PTIHH30 pKa = 6.25MGNNIFKK37 pKa = 10.61NITILLIGQPILFMFLIAFNTFIIAFLVFIACVIIYY73 pKa = 9.35QIFTWIMKK81 pKa = 10.18GNN83 pKa = 3.54

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

189

0

189

36423

29

3275

192.7

22.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.884 ± 0.334

0.615 ± 0.082

6.902 ± 0.175

8.135 ± 0.293

4.478 ± 0.245

5.768 ± 0.214

1.34 ± 0.1

7.72 ± 0.177

9.027 ± 0.164

8.237 ± 0.173

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.104

6.455 ± 0.185

2.227 ± 0.104

3.3 ± 0.311

3.476 ± 0.147

6.625 ± 0.261

6.227 ± 0.212

6.221 ± 0.166

1.093 ± 0.088

4.588 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski