Dishui lake virophage 1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141HRM6|A0A141HRM6_9VIRU Uncharacterized protein OS=Dishui lake virophage 1 OX=1739971 PE=4 SV=1
MM1 pKa = 7.24SVSSILNPATGKK13 pKa = 10.33IYY15 pKa = 10.61DD16 pKa = 4.26DD17 pKa = 4.94LVPQGGGVPLAKK29 pKa = 10.36GGLITANAGGAEE41 pKa = 3.98QALAVGNDD49 pKa = 3.12GWVLSADD56 pKa = 3.74SNEE59 pKa = 3.98ALGLKK64 pKa = 9.55YY65 pKa = 10.16IQLPAGGIQFTQQGQLLYY83 pKa = 10.68AGAGPAFNDD92 pKa = 3.3SLLNIGNAGQILGINAGIPAWINAGGSGTITALAPLTEE130 pKa = 4.45YY131 pKa = 11.54ADD133 pKa = 3.79GTASKK138 pKa = 10.69VAVNFTGKK146 pKa = 10.69GDD148 pKa = 4.61LIVGGGVQVGGEE160 pKa = 4.03PVAGVILPVGANDD173 pKa = 3.57MVLTANSATASGLEE187 pKa = 4.12WKK189 pKa = 10.59ASGGGSSATIFRR201 pKa = 11.84NSVDD205 pKa = 3.72DD206 pKa = 3.94TPLVITKK213 pKa = 9.1PATANDD219 pKa = 3.93TCVITADD226 pKa = 3.99RR227 pKa = 11.84TYY229 pKa = 10.78HH230 pKa = 7.0AYY232 pKa = 8.06NTQIYY237 pKa = 9.45SKK239 pKa = 10.37GGVGTQVDD247 pKa = 4.09MTNPQPFPFFTWTAPNDD264 pKa = 3.38INMTSVSSQVKK275 pKa = 9.99IITNSIYY282 pKa = 10.65GQTVHH287 pKa = 7.89LDD289 pKa = 3.3IYY291 pKa = 10.97DD292 pKa = 3.81STGTTGLVGGDD303 pKa = 3.66PVNVSDD309 pKa = 5.68NEE311 pKa = 4.15PAGDD315 pKa = 3.82AVSLPITTAFQFVNGTTYY333 pKa = 11.16QFMVSLDD340 pKa = 3.69GLDD343 pKa = 3.16QGEE346 pKa = 4.35FFDD349 pKa = 4.19MFIDD353 pKa = 4.56GGQYY357 pKa = 10.2VGNITINGVDD367 pKa = 4.11YY368 pKa = 11.45ANLPATFTLAPPYY381 pKa = 10.06KK382 pKa = 10.26FRR384 pKa = 11.84VSNDD388 pKa = 3.18LTGKK392 pKa = 8.94TSATCEE398 pKa = 4.13NFSSQSFVASEE409 pKa = 4.52DD410 pKa = 3.83TNSWIAVGGINAGVAFVPP428 pKa = 4.51

Molecular weight: 43.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141HRN6|A0A141HRN6_9VIRU Uncharacterized protein OS=Dishui lake virophage 1 OX=1739971 PE=4 SV=1
MM1 pKa = 7.32LCSYY5 pKa = 10.27SKK7 pKa = 10.95KK8 pKa = 10.46SFSATLPSRR17 pKa = 11.84DD18 pKa = 3.35FSTASFNSSTLRR30 pKa = 11.84SITDD34 pKa = 3.36YY35 pKa = 10.98CSSARR40 pKa = 11.84FLIISLNASSCVGSAFRR57 pKa = 11.84FFQNLIHH64 pKa = 6.85SGRR67 pKa = 11.84KK68 pKa = 6.59YY69 pKa = 10.55HH70 pKa = 6.99ISLFII75 pKa = 4.92

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28

0

28

8703

63

857

310.8

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.836 ± 0.412

1.126 ± 0.189

5.538 ± 0.355

5.872 ± 0.685

3.976 ± 0.214

8.307 ± 1.191

1.241 ± 0.185

5.607 ± 0.376

6.71 ± 0.731

8.009 ± 0.345

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.873 ± 0.214

5.676 ± 0.253

4.998 ± 0.467

4.929 ± 0.491

4.309 ± 0.548

7.055 ± 0.592

6.182 ± 0.685

5.607 ± 0.375

0.954 ± 0.121

4.194 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski