Arctopus echinatus-associated virus
Average proteome isoelectric point is 7.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345BKA2|A0A345BKA2_9VIRU Replication-associated protein OS=Arctopus echinatus-associated virus OX=2282642 PE=3 SV=1
MM1 pKa = 7.64 SSGSTNQLLTLRR13 pKa = 11.84 GRR15 pKa = 11.84 YY16 pKa = 8.63 FLLTYY21 pKa = 9.86 PRR23 pKa = 11.84 SDD25 pKa = 3.67 FNHH28 pKa = 4.73 AHH30 pKa = 6.59 AWIQLNEE37 pKa = 4.11 RR38 pKa = 11.84 GACGAIVCSEE48 pKa = 3.91 LHH50 pKa = 6.27 QDD52 pKa = 3.78 DD53 pKa = 4.24 TEE55 pKa = 4.22 HH56 pKa = 6.72 RR57 pKa = 11.84 HH58 pKa = 5.07 IAVDD62 pKa = 4.1 FGRR65 pKa = 11.84 RR66 pKa = 11.84 RR67 pKa = 11.84 QFSNAHH73 pKa = 5.01 HH74 pKa = 6.65 TFNITLGDD82 pKa = 3.89 TTWTGNYY89 pKa = 5.64 QTARR93 pKa = 11.84 SWAAIRR99 pKa = 11.84 HH100 pKa = 4.5 YY101 pKa = 10.42 VEE103 pKa = 4.45 KK104 pKa = 10.44 DD105 pKa = 3.23 GNVSYY110 pKa = 10.85 FGTSEE115 pKa = 4.2 PNLEE119 pKa = 4.08 QANPEE124 pKa = 4.28 TEE126 pKa = 4.07 TEE128 pKa = 4.45 DD129 pKa = 4.64 IIDD132 pKa = 3.94 ALTRR136 pKa = 11.84 TQSYY140 pKa = 10.91 AEE142 pKa = 3.97 WLVWTIKK149 pKa = 10.51 QKK151 pKa = 10.76 ISFGHH156 pKa = 7.1 ANALWQEE163 pKa = 4.01 LRR165 pKa = 11.84 GNRR168 pKa = 11.84 PATLLEE174 pKa = 4.32 GDD176 pKa = 4.53 NIPGNVVLPEE186 pKa = 3.91 LQLRR190 pKa = 11.84 TFSQDD195 pKa = 2.72 TNRR198 pKa = 11.84 ALVLLGPSGVGKK210 pKa = 6.77 TTWAKK215 pKa = 11.0 LNIPKK220 pKa = 9.67 PALLVSHH227 pKa = 7.25 IDD229 pKa = 3.54 DD230 pKa = 4.2 LKK232 pKa = 9.05 HH233 pKa = 6.31 FKK235 pKa = 10.93 VGVHH239 pKa = 6.02 KK240 pKa = 10.85 GIIFDD245 pKa = 4.34 DD246 pKa = 3.43 MHH248 pKa = 6.75 FAGDD252 pKa = 3.97 EE253 pKa = 4.12 NGKK256 pKa = 9.54 GAWPRR261 pKa = 11.84 TSQIHH266 pKa = 6.24 LVDD269 pKa = 4.46 FEE271 pKa = 4.46 NPRR274 pKa = 11.84 SIHH277 pKa = 5.65 CRR279 pKa = 11.84 HH280 pKa = 6.28 RR281 pKa = 11.84 CATIPAGVFKK291 pKa = 10.83 IFTCNVYY298 pKa = 10.25 PFSVDD303 pKa = 3.15 PAIEE307 pKa = 3.82 RR308 pKa = 11.84 RR309 pKa = 11.84 IYY311 pKa = 10.83 LITLNFLQQ319 pKa = 4.65
Molecular weight: 36.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.566
IPC2_protein 6.465
IPC_protein 6.634
Toseland 6.883
ProMoST 6.971
Dawson 7.059
Bjellqvist 6.971
Wikipedia 7.059
Rodwell 7.044
Grimsley 7.029
Solomon 7.073
Lehninger 7.073
Nozaki 7.307
DTASelect 7.439
Thurlkill 7.483
EMBOSS 7.483
Sillero 7.454
Patrickios 4.355
IPC_peptide 7.088
IPC2_peptide 7.205
IPC2.peptide.svr19 6.976
Protein with the highest isoelectric point:
>tr|A0A345BKA2|A0A345BKA2_9VIRU Replication-associated protein OS=Arctopus echinatus-associated virus OX=2282642 PE=3 SV=1
MM1 pKa = 7.8 AKK3 pKa = 10.03 RR4 pKa = 11.84 SRR6 pKa = 11.84 SRR8 pKa = 11.84 SVSRR12 pKa = 11.84 GRR14 pKa = 11.84 RR15 pKa = 11.84 KK16 pKa = 10.19 LPSNWHH22 pKa = 5.35 MASNVARR29 pKa = 11.84 AAGTAYY35 pKa = 10.42 DD36 pKa = 3.32 AYY38 pKa = 10.07 KK39 pKa = 10.17 RR40 pKa = 11.84 VKK42 pKa = 9.87 SRR44 pKa = 11.84 SSSRR48 pKa = 11.84 VKK50 pKa = 10.63 ASRR53 pKa = 11.84 SSSATRR59 pKa = 11.84 TRR61 pKa = 11.84 TRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 GPSAGSAGTDD75 pKa = 3.26 GSVSSFRR82 pKa = 11.84 YY83 pKa = 8.54 SVRR86 pKa = 11.84 RR87 pKa = 11.84 GVKK90 pKa = 8.91 VPKK93 pKa = 10.41 GFRR96 pKa = 11.84 KK97 pKa = 10.0 LLAPRR102 pKa = 11.84 HH103 pKa = 5.22 MNSVAFSKK111 pKa = 9.27 ITSAVNRR118 pKa = 11.84 QGVNMVNLFNNGLAPANGTVVEE140 pKa = 4.62 AVPSFLDD147 pKa = 3.56 TEE149 pKa = 4.82 LMMTNLLSDD158 pKa = 4.13 EE159 pKa = 4.63 AATSVGVNTIRR170 pKa = 11.84 YY171 pKa = 6.73 WIPFISLKK179 pKa = 8.87 ITFRR183 pKa = 11.84 SMTSSPVHH191 pKa = 5.67 LKK193 pKa = 10.13 IYY195 pKa = 9.91 NCVARR200 pKa = 11.84 RR201 pKa = 11.84 DD202 pKa = 3.98 QEE204 pKa = 3.9 WRR206 pKa = 11.84 PTVAFNMANSPYY218 pKa = 7.85 TTWSAGLTDD227 pKa = 3.76 EE228 pKa = 5.36 FGPGSTPTLTVPGTTPFQSEE248 pKa = 5.31 SFCQWWNVKK257 pKa = 8.69 SSKK260 pKa = 10.37 FVMLHH265 pKa = 6.31 PGAEE269 pKa = 3.93 HH270 pKa = 4.89 VHH272 pKa = 6.03 FVRR275 pKa = 11.84 IQLNRR280 pKa = 11.84 LHH282 pKa = 6.64 NRR284 pKa = 11.84 ALTNDD289 pKa = 3.72 LAAIARR295 pKa = 11.84 EE296 pKa = 4.56 SYY298 pKa = 11.08 ACMAVVRR305 pKa = 11.84 GGVVQDD311 pKa = 5.34 AIATGNVGYY320 pKa = 10.67 GPATVDD326 pKa = 3.49 VMCDD330 pKa = 3.07 YY331 pKa = 11.28 HH332 pKa = 7.88 MEE334 pKa = 4.14 AQAVGKK340 pKa = 9.97 NRR342 pKa = 11.84 TIVSQYY348 pKa = 10.62 QYY350 pKa = 11.21 DD351 pKa = 3.96 GAITGTGQQVAEE363 pKa = 4.52 DD364 pKa = 4.5 TDD366 pKa = 3.6 TGAPVVNLGG375 pKa = 3.57
Molecular weight: 40.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.75
IPC_protein 10.57
Toseland 10.57
ProMoST 10.306
Dawson 10.701
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.906
Grimsley 10.774
Solomon 10.789
Lehninger 10.76
Nozaki 10.555
DTASelect 10.423
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.57
IPC_peptide 10.789
IPC2_peptide 9.458
IPC2.peptide.svr19 8.527
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
694
319
375
347.0
38.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.79 ± 1.026
1.297 ± 0.176
4.323 ± 0.654
3.746 ± 0.825
4.179 ± 0.544
7.205 ± 0.2
3.458 ± 1.012
4.611 ± 1.282
3.602 ± 0.1
6.916 ± 1.413
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.017 ± 0.903
5.764 ± 0.125
4.467 ± 0.051
3.458 ± 0.401
7.781 ± 0.778
8.069 ± 1.781
7.925 ± 0.057
7.637 ± 1.703
2.017 ± 0.319
2.738 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here