Streptococcus phage phi-m46.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0R0G0|D0R0G0_9CAUD Phosphorylase OS=Streptococcus phage phi-m46.1 OX=571950 GN=pnp PE=4 SV=1
MM1 pKa = 7.6PVQMDD6 pKa = 4.13GVSEE10 pKa = 4.54HH11 pKa = 6.25EE12 pKa = 4.17LSRR15 pKa = 11.84LGSKK19 pKa = 9.21MVDD22 pKa = 3.26IINSDD27 pKa = 3.87FIDD30 pKa = 3.82YY31 pKa = 10.51KK32 pKa = 11.35DD33 pKa = 3.85LVGSSEE39 pKa = 3.82YY40 pKa = 10.98SIIKK44 pKa = 10.47DD45 pKa = 3.21GGSYY49 pKa = 10.37PILDD53 pKa = 4.89LPCQEE58 pKa = 4.98CGEE61 pKa = 4.1YY62 pKa = 9.53WICIDD67 pKa = 4.73EE68 pKa = 4.51AFTDD72 pKa = 3.66RR73 pKa = 11.84GKK75 pKa = 10.88CLNCGEE81 pKa = 4.36INEE84 pKa = 4.35VTGCEE89 pKa = 3.76RR90 pKa = 11.84CGGYY94 pKa = 10.7DD95 pKa = 3.41FGTPSDD101 pKa = 3.55YY102 pKa = 10.81DD103 pKa = 3.78YY104 pKa = 11.65PFLCDD109 pKa = 3.37SCCNYY114 pKa = 10.4YY115 pKa = 10.4KK116 pKa = 10.9EE117 pKa = 4.25EE118 pKa = 4.0

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0R0B2|D0R0B2_9CAUD Uncharacterized protein OS=Streptococcus phage phi-m46.1 OX=571950 PE=4 SV=1
MM1 pKa = 7.72MYY3 pKa = 10.16TILTCTIMGLWVLIGLYY20 pKa = 9.77FGYY23 pKa = 8.1MTIRR27 pKa = 11.84DD28 pKa = 4.32DD29 pKa = 3.97IRR31 pKa = 11.84NEE33 pKa = 3.5MEE35 pKa = 3.56RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 10.13RR40 pKa = 11.84KK41 pKa = 9.52KK42 pKa = 10.05EE43 pKa = 3.83KK44 pKa = 10.45LSQIPLSRR52 pKa = 11.84KK53 pKa = 9.39NKK55 pKa = 9.63

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

16668

51

1044

256.4

28.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.509 ± 0.31

1.032 ± 0.143

5.586 ± 0.268

7.439 ± 0.341

4.002 ± 0.177

6.389 ± 0.233

1.896 ± 0.125

6.947 ± 0.325

7.223 ± 0.257

9.077 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.14

4.698 ± 0.187

3.126 ± 0.186

4.416 ± 0.214

4.656 ± 0.216

6.899 ± 0.34

6.216 ± 0.342

6.389 ± 0.197

1.338 ± 0.095

3.738 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski