Mycobacterium phage PainterBoy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LF40|A0A7G8LF40_9CAUD DUF2786 domain-containing protein OS=Mycobacterium phage PainterBoy OX=2762425 GN=83 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.36PVKK6 pKa = 10.63EE7 pKa = 4.24FAEE10 pKa = 4.43VWSDD14 pKa = 3.22GNLAYY19 pKa = 9.92EE20 pKa = 4.18VGPTLSCTEE29 pKa = 4.04AEE31 pKa = 4.37ALASLLLGIGKK42 pKa = 9.05PEE44 pKa = 4.41AAEE47 pKa = 3.77AWINSHH53 pKa = 6.9ASSDD57 pKa = 3.74DD58 pKa = 3.64CGDD61 pKa = 3.45IHH63 pKa = 6.29CTCSDD68 pKa = 3.62CHH70 pKa = 7.22PEE72 pKa = 4.0VTISTEE78 pKa = 3.98SPKK81 pKa = 11.08

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LF39|A0A7G8LF39_9CAUD Uncharacterized protein OS=Mycobacterium phage PainterBoy OX=2762425 GN=81 PE=4 SV=1
MM1 pKa = 7.46SGKK4 pKa = 7.38TQQSGSFRR12 pKa = 11.84APLIFSVIEE21 pKa = 3.67DD22 pKa = 4.05LRR24 pKa = 11.84RR25 pKa = 11.84KK26 pKa = 10.02GYY28 pKa = 8.98NQSEE32 pKa = 4.44IADD35 pKa = 3.58MHH37 pKa = 6.18GVTRR41 pKa = 11.84QAVSWQKK48 pKa = 9.05KK49 pKa = 6.6TYY51 pKa = 8.76GGRR54 pKa = 11.84MTPRR58 pKa = 11.84DD59 pKa = 3.7IVRR62 pKa = 11.84EE63 pKa = 3.94AWPFEE68 pKa = 3.99TTNLHH73 pKa = 5.82GKK75 pKa = 8.9SVPFQRR81 pKa = 11.84LRR83 pKa = 11.84DD84 pKa = 3.71HH85 pKa = 6.99GEE87 pKa = 3.83FMATGEE93 pKa = 4.1RR94 pKa = 11.84RR95 pKa = 11.84HH96 pKa = 5.85EE97 pKa = 4.25RR98 pKa = 11.84KK99 pKa = 9.5QDD101 pKa = 3.16TTTEE105 pKa = 4.22GVVAKK110 pKa = 10.36APRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84CAA118 pKa = 3.14

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

16303

34

1020

175.3

19.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.612 ± 0.335

0.945 ± 0.129

6.563 ± 0.161

6.864 ± 0.28

3.325 ± 0.154

8.115 ± 0.481

2.03 ± 0.183

5.067 ± 0.199

4.84 ± 0.274

7.913 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.147

3.325 ± 0.16

5.576 ± 0.254

3.435 ± 0.198

6.373 ± 0.274

5.931 ± 0.197

5.931 ± 0.208

7.226 ± 0.227

1.938 ± 0.134

2.662 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski