Amino acid dipepetide frequency for Helicobacter sp. MIT 01-6242

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.815AlaAla: 1.815 ± 0.114
0.9AlaCys: 0.9 ± 0.05
2.853AlaAsp: 2.853 ± 0.078
3.133AlaGlu: 3.133 ± 0.086
3.549AlaPhe: 3.549 ± 0.095
4.111AlaGly: 4.111 ± 0.109
1.484AlaHis: 1.484 ± 0.058
5.903AlaIle: 5.903 ± 0.117
6.049AlaLys: 6.049 ± 0.141
8.983AlaLeu: 8.983 ± 0.163
1.898AlaMet: 1.898 ± 0.059
3.719AlaAsn: 3.719 ± 0.109
2.197AlaPro: 2.197 ± 0.075
4.175AlaGln: 4.175 ± 0.096
2.781AlaArg: 2.781 ± 0.074
5.156AlaSer: 5.156 ± 0.127
4.124AlaThr: 4.124 ± 0.195
3.211AlaVal: 3.211 ± 0.08
0.45AlaTrp: 0.45 ± 0.035
2.74AlaTyr: 2.74 ± 0.079
0.0AlaXaa: 0.0 ± 0.0
Cys
0.972CysAla: 0.972 ± 0.05
0.185CysCys: 0.185 ± 0.018
0.8CysAsp: 0.8 ± 0.046
0.79CysGlu: 0.79 ± 0.042
0.804CysPhe: 0.804 ± 0.047
1.14CysGly: 1.14 ± 0.06
0.265CysHis: 0.265 ± 0.029
0.97CysIle: 0.97 ± 0.051
0.855CysLys: 0.855 ± 0.044
1.116CysLeu: 1.116 ± 0.051
0.212CysMet: 0.212 ± 0.025
0.554CysAsn: 0.554 ± 0.033
0.45CysPro: 0.45 ± 0.029
0.554CysGln: 0.554 ± 0.035
0.363CysArg: 0.363 ± 0.03
0.728CysSer: 0.728 ± 0.044
0.418CysThr: 0.418 ± 0.03
0.995CysVal: 0.995 ± 0.052
0.093CysTrp: 0.093 ± 0.015
0.473CysTyr: 0.473 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
2.921AspAla: 2.921 ± 0.085
0.836AspCys: 0.836 ± 0.05
1.925AspAsp: 1.925 ± 0.076
3.402AspGlu: 3.402 ± 0.1
3.649AspPhe: 3.649 ± 0.099
3.207AspGly: 3.207 ± 0.09
0.754AspHis: 0.754 ± 0.039
3.661AspIle: 3.661 ± 0.093
3.443AspLys: 3.443 ± 0.102
5.362AspLeu: 5.362 ± 0.104
1.102AspMet: 1.102 ± 0.058
2.072AspAsn: 2.072 ± 0.075
1.467AspPro: 1.467 ± 0.053
1.388AspGln: 1.388 ± 0.053
1.785AspArg: 1.785 ± 0.067
3.528AspSer: 3.528 ± 0.083
2.728AspThr: 2.728 ± 0.091
2.791AspVal: 2.791 ± 0.098
0.469AspTrp: 0.469 ± 0.034
2.15AspTyr: 2.15 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
4.693GluAla: 4.693 ± 0.109
0.991GluCys: 0.991 ± 0.057
2.607GluAsp: 2.607 ± 0.082
4.674GluGlu: 4.674 ± 0.122
3.129GluPhe: 3.129 ± 0.089
3.995GluGly: 3.995 ± 0.119
1.104GluHis: 1.104 ± 0.043
6.402GluIle: 6.402 ± 0.128
4.856GluLys: 4.856 ± 0.127
6.037GluLeu: 6.037 ± 0.13
1.734GluMet: 1.734 ± 0.071
3.543GluAsn: 3.543 ± 0.094
1.172GluPro: 1.172 ± 0.052
2.63GluGln: 2.63 ± 0.08
2.643GluArg: 2.643 ± 0.073
3.912GluSer: 3.912 ± 0.11
2.35GluThr: 2.35 ± 0.076
4.761GluVal: 4.761 ± 0.112
0.554GluTrp: 0.554 ± 0.039
2.21GluTyr: 2.21 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
4.332PheAla: 4.332 ± 0.116
0.942PheCys: 0.942 ± 0.051
3.184PheAsp: 3.184 ± 0.09
3.587PheGlu: 3.587 ± 0.106
2.751PhePhe: 2.751 ± 0.113
4.247PheGly: 4.247 ± 0.096
0.811PheHis: 0.811 ± 0.047
2.991PheIle: 2.991 ± 0.097
2.891PheLys: 2.891 ± 0.079
5.659PheLeu: 5.659 ± 0.145
1.01PheMet: 1.01 ± 0.048
2.396PheAsn: 2.396 ± 0.107
1.291PhePro: 1.291 ± 0.052
1.745PheGln: 1.745 ± 0.067
1.378PheArg: 1.378 ± 0.059
3.301PheSer: 3.301 ± 0.099
1.944PheThr: 1.944 ± 0.076
3.882PheVal: 3.882 ± 0.123
0.537PheTrp: 0.537 ± 0.043
2.116PheTyr: 2.116 ± 0.079
0.0PheXaa: 0.0 ± 0.0
Gly
4.999GlyAla: 4.999 ± 0.149
0.758GlyCys: 0.758 ± 0.051
3.568GlyAsp: 3.568 ± 0.109
4.557GlyGlu: 4.557 ± 0.109
3.627GlyPhe: 3.627 ± 0.106
6.206GlyGly: 6.206 ± 0.212
1.129GlyHis: 1.129 ± 0.052
5.994GlyIle: 5.994 ± 0.124
4.873GlyLys: 4.873 ± 0.114
5.614GlyLeu: 5.614 ± 0.112
1.592GlyMet: 1.592 ± 0.066
3.56GlyAsn: 3.56 ± 0.187
0.705GlyPro: 0.705 ± 0.041
1.847GlyGln: 1.847 ± 0.07
2.352GlyArg: 2.352 ± 0.075
4.568GlySer: 4.568 ± 0.133
3.536GlyThr: 3.536 ± 0.182
5.243GlyVal: 5.243 ± 0.145
0.569GlyTrp: 0.569 ± 0.037
2.753GlyTyr: 2.753 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
0.868HisAla: 0.868 ± 0.037
0.34HisCys: 0.34 ± 0.029
0.507HisAsp: 0.507 ± 0.035
0.444HisGlu: 0.444 ± 0.03
1.39HisPhe: 1.39 ± 0.061
0.811HisGly: 0.811 ± 0.04
0.66HisHis: 0.66 ± 0.042
1.974HisIle: 1.974 ± 0.072
1.456HisLys: 1.456 ± 0.058
2.526HisLeu: 2.526 ± 0.086
0.267HisMet: 0.267 ± 0.028
1.112HisAsn: 1.112 ± 0.052
0.917HisPro: 0.917 ± 0.046
1.39HisGln: 1.39 ± 0.057
0.817HisArg: 0.817 ± 0.048
1.802HisSer: 1.802 ± 0.061
1.452HisThr: 1.452 ± 0.065
0.231HisVal: 0.231 ± 0.024
0.144HisTrp: 0.144 ± 0.021
0.951HisTyr: 0.951 ± 0.052
0.0HisXaa: 0.0 ± 0.0
Ile
7.427IleAla: 7.427 ± 0.136
1.104IleCys: 1.104 ± 0.048
4.53IleAsp: 4.53 ± 0.104
5.192IleGlu: 5.192 ± 0.123
3.829IlePhe: 3.829 ± 0.114
5.472IleGly: 5.472 ± 0.129
1.477IleHis: 1.477 ± 0.059
5.27IleIle: 5.27 ± 0.137
5.979IleLys: 5.979 ± 0.124
8.376IleLeu: 8.376 ± 0.164
1.11IleMet: 1.11 ± 0.054
3.78IleAsn: 3.78 ± 0.11
3.114IlePro: 3.114 ± 0.098
3.625IleGln: 3.625 ± 0.102
2.456IleArg: 2.456 ± 0.08
4.99IleSer: 4.99 ± 0.126
4.68IleThr: 4.68 ± 0.144
4.789IleVal: 4.789 ± 0.108
0.529IleTrp: 0.529 ± 0.041
2.753IleTyr: 2.753 ± 0.082
0.0IleXaa: 0.0 ± 0.0
Lys
5.188LysAla: 5.188 ± 0.117
0.62LysCys: 0.62 ± 0.037
3.942LysAsp: 3.942 ± 0.099
6.217LysGlu: 6.217 ± 0.158
2.237LysPhe: 2.237 ± 0.083
4.279LysGly: 4.279 ± 0.139
1.505LysHis: 1.505 ± 0.061
7.0LysIle: 7.0 ± 0.119
5.534LysLys: 5.534 ± 0.153
6.425LysLeu: 6.425 ± 0.119
2.053LysMet: 2.053 ± 0.077
4.536LysAsn: 4.536 ± 0.118
2.418LysPro: 2.418 ± 0.075
3.146LysGln: 3.146 ± 0.098
3.118LysArg: 3.118 ± 0.081
4.288LysSer: 4.288 ± 0.104
4.107LysThr: 4.107 ± 0.096
3.708LysVal: 3.708 ± 0.106
0.499LysTrp: 0.499 ± 0.04
2.176LysTyr: 2.176 ± 0.07
0.0LysXaa: 0.0 ± 0.0
Leu
7.181LeuAla: 7.181 ± 0.133
1.554LeuCys: 1.554 ± 0.066
5.638LeuAsp: 5.638 ± 0.109
7.958LeuGlu: 7.958 ± 0.147
4.803LeuPhe: 4.803 ± 0.145
7.121LeuGly: 7.121 ± 0.146
2.084LeuHis: 2.084 ± 0.069
6.796LeuIle: 6.796 ± 0.154
8.439LeuLys: 8.439 ± 0.144
9.662LeuLeu: 9.662 ± 0.219
2.05LeuMet: 2.05 ± 0.076
5.215LeuAsn: 5.215 ± 0.12
3.619LeuPro: 3.619 ± 0.101
5.593LeuGln: 5.593 ± 0.109
3.937LeuArg: 3.937 ± 0.096
7.904LeuSer: 7.904 ± 0.123
4.867LeuThr: 4.867 ± 0.171
5.222LeuVal: 5.222 ± 0.123
0.794LeuTrp: 0.794 ± 0.043
3.415LeuTyr: 3.415 ± 0.082
0.0LeuXaa: 0.0 ± 0.0
Met
1.356MetAla: 1.356 ± 0.058
0.274MetCys: 0.274 ± 0.027
0.855MetAsp: 0.855 ± 0.045
1.155MetGlu: 1.155 ± 0.052
0.962MetPhe: 0.962 ± 0.067
1.549MetGly: 1.549 ± 0.068
0.399MetHis: 0.399 ± 0.03
1.925MetIle: 1.925 ± 0.073
1.56MetLys: 1.56 ± 0.062
2.464MetLeu: 2.464 ± 0.091
0.599MetMet: 0.599 ± 0.039
0.813MetAsn: 0.813 ± 0.046
0.932MetPro: 0.932 ± 0.051
1.443MetGln: 1.443 ± 0.058
1.246MetArg: 1.246 ± 0.058
1.494MetSer: 1.494 ± 0.064
0.866MetThr: 0.866 ± 0.047
1.119MetVal: 1.119 ± 0.051
0.144MetTrp: 0.144 ± 0.017
0.433MetTyr: 0.433 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
4.451AsnAla: 4.451 ± 0.133
0.348AsnCys: 0.348 ± 0.03
2.182AsnAsp: 2.182 ± 0.072
2.549AsnGlu: 2.549 ± 0.086
2.579AsnPhe: 2.579 ± 0.089
4.145AsnGly: 4.145 ± 0.184
1.248AsnHis: 1.248 ± 0.057
4.3AsnIle: 4.3 ± 0.139
3.063AsnLys: 3.063 ± 0.089
5.952AsnLeu: 5.952 ± 0.139
0.787AsnMet: 0.787 ± 0.046
2.46AsnAsn: 2.46 ± 0.125
2.598AsnPro: 2.598 ± 0.07
2.885AsnGln: 2.885 ± 0.089
1.7AsnArg: 1.7 ± 0.064
2.662AsnSer: 2.662 ± 0.119
3.048AsnThr: 3.048 ± 0.126
2.774AsnVal: 2.774 ± 0.114
0.227AsnTrp: 0.227 ± 0.03
1.632AsnTyr: 1.632 ± 0.059
0.0AsnXaa: 0.0 ± 0.0
Pro
1.513ProAla: 1.513 ± 0.059
0.354ProCys: 0.354 ± 0.027
1.144ProAsp: 1.144 ± 0.049
1.297ProGlu: 1.297 ± 0.053
1.753ProPhe: 1.753 ± 0.061
0.868ProGly: 0.868 ± 0.046
0.93ProHis: 0.93 ± 0.046
2.77ProIle: 2.77 ± 0.082
3.029ProLys: 3.029 ± 0.095
3.695ProLeu: 3.695 ± 0.103
0.7ProMet: 0.7 ± 0.041
2.042ProAsn: 2.042 ± 0.064
1.369ProPro: 1.369 ± 0.072
2.201ProGln: 2.201 ± 0.07
0.993ProArg: 0.993 ± 0.047
2.505ProSer: 2.505 ± 0.077
2.142ProThr: 2.142 ± 0.072
1.354ProVal: 1.354 ± 0.061
0.231ProTrp: 0.231 ± 0.024
1.462ProTyr: 1.462 ± 0.059
0.0ProXaa: 0.0 ± 0.0
Gln
3.46GlnAla: 3.46 ± 0.098
0.425GlnCys: 0.425 ± 0.032
2.74GlnAsp: 2.74 ± 0.082
4.835GlnGlu: 4.835 ± 0.106
1.365GlnPhe: 1.365 ± 0.061
2.723GlnGly: 2.723 ± 0.089
0.86GlnHis: 0.86 ± 0.041
4.194GlnIle: 4.194 ± 0.116
4.568GlnLys: 4.568 ± 0.113
2.615GlnLeu: 2.615 ± 0.076
1.174GlnMet: 1.174 ± 0.044
3.392GlnAsn: 3.392 ± 0.086
0.959GlnPro: 0.959 ± 0.061
1.959GlnGln: 1.959 ± 0.08
1.7GlnArg: 1.7 ± 0.066
3.426GlnSer: 3.426 ± 0.088
3.139GlnThr: 3.139 ± 0.088
2.05GlnVal: 2.05 ± 0.077
0.359GlnTrp: 0.359 ± 0.028
1.382GlnTyr: 1.382 ± 0.058
0.0GlnXaa: 0.0 ± 0.0
Arg
2.432ArgAla: 2.432 ± 0.075
0.374ArgCys: 0.374 ± 0.029
1.915ArgAsp: 1.915 ± 0.063
2.738ArgGlu: 2.738 ± 0.088
2.388ArgPhe: 2.388 ± 0.069
2.511ArgGly: 2.511 ± 0.082
0.737ArgHis: 0.737 ± 0.044
3.415ArgIle: 3.415 ± 0.103
2.324ArgLys: 2.324 ± 0.078
3.706ArgLeu: 3.706 ± 0.108
0.894ArgMet: 0.894 ± 0.043
1.857ArgAsn: 1.857 ± 0.056
0.875ArgPro: 0.875 ± 0.045
1.367ArgGln: 1.367 ± 0.053
1.314ArgArg: 1.314 ± 0.064
2.186ArgSer: 2.186 ± 0.063
1.448ArgThr: 1.448 ± 0.055
2.543ArgVal: 2.543 ± 0.072
0.297ArgTrp: 0.297 ± 0.028
1.615ArgTyr: 1.615 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
4.786SerAla: 4.786 ± 0.115
0.724SerCys: 0.724 ± 0.042
3.018SerAsp: 3.018 ± 0.09
2.974SerGlu: 2.974 ± 0.082
3.855SerPhe: 3.855 ± 0.101
5.02SerGly: 5.02 ± 0.143
1.405SerHis: 1.405 ± 0.053
5.207SerIle: 5.207 ± 0.104
4.799SerLys: 4.799 ± 0.107
7.55SerLeu: 7.55 ± 0.142
1.439SerMet: 1.439 ± 0.06
3.579SerAsn: 3.579 ± 0.144
2.163SerPro: 2.163 ± 0.08
3.262SerGln: 3.262 ± 0.086
2.246SerArg: 2.246 ± 0.079
4.969SerSer: 4.969 ± 0.148
3.494SerThr: 3.494 ± 0.136
4.349SerVal: 4.349 ± 0.11
0.444SerTrp: 0.444 ± 0.033
2.524SerTyr: 2.524 ± 0.067
0.0SerXaa: 0.0 ± 0.0
Thr
3.12ThrAla: 3.12 ± 0.145
0.403ThrCys: 0.403 ± 0.033
2.125ThrAsp: 2.125 ± 0.084
2.103ThrGlu: 2.103 ± 0.07
2.545ThrPhe: 2.545 ± 0.101
2.774ThrGly: 2.774 ± 0.164
1.479ThrHis: 1.479 ± 0.063
4.387ThrIle: 4.387 ± 0.168
3.33ThrLys: 3.33 ± 0.105
7.374ThrLeu: 7.374 ± 0.214
0.889ThrMet: 0.889 ± 0.048
2.802ThrAsn: 2.802 ± 0.147
2.999ThrPro: 2.999 ± 0.083
3.829ThrGln: 3.829 ± 0.102
1.923ThrArg: 1.923 ± 0.06
3.81ThrSer: 3.81 ± 0.144
3.496ThrThr: 3.496 ± 0.221
0.647ThrVal: 0.647 ± 0.044
0.263ThrTrp: 0.263 ± 0.023
1.698ThrTyr: 1.698 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
4.241ValAla: 4.241 ± 0.1
0.974ValCys: 0.974 ± 0.051
2.817ValAsp: 2.817 ± 0.085
3.655ValGlu: 3.655 ± 0.102
3.352ValPhe: 3.352 ± 0.091
4.721ValGly: 4.721 ± 0.122
0.737ValHis: 0.737 ± 0.042
4.235ValIle: 4.235 ± 0.101
3.19ValLys: 3.19 ± 0.103
6.266ValLeu: 6.266 ± 0.138
1.341ValMet: 1.341 ± 0.059
2.042ValAsn: 2.042 ± 0.073
1.564ValPro: 1.564 ± 0.07
2.019ValGln: 2.019 ± 0.06
2.231ValArg: 2.231 ± 0.082
4.071ValSer: 4.071 ± 0.097
2.082ValThr: 2.082 ± 0.108
3.923ValVal: 3.923 ± 0.112
0.571ValTrp: 0.571 ± 0.033
1.862ValTyr: 1.862 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.531TrpAla: 0.531 ± 0.038
0.144TrpCys: 0.144 ± 0.017
0.399TrpAsp: 0.399 ± 0.042
0.529TrpGlu: 0.529 ± 0.033
0.384TrpPhe: 0.384 ± 0.031
0.645TrpGly: 0.645 ± 0.039
0.18TrpHis: 0.18 ± 0.023
0.643TrpIle: 0.643 ± 0.042
0.437TrpLys: 0.437 ± 0.033
0.841TrpLeu: 0.841 ± 0.046
0.174TrpMet: 0.174 ± 0.021
0.391TrpAsn: 0.391 ± 0.034
0.064TrpPro: 0.064 ± 0.013
0.391TrpGln: 0.391 ± 0.029
0.333TrpArg: 0.333 ± 0.029
0.393TrpSer: 0.393 ± 0.028
0.236TrpThr: 0.236 ± 0.026
0.495TrpVal: 0.495 ± 0.037
0.089TrpTrp: 0.089 ± 0.016
0.242TrpTyr: 0.242 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.944TyrAla: 2.944 ± 0.084
0.49TyrCys: 0.49 ± 0.033
1.891TyrAsp: 1.891 ± 0.065
2.205TyrGlu: 2.205 ± 0.067
2.044TyrPhe: 2.044 ± 0.077
2.503TyrGly: 2.503 ± 0.075
0.813TyrHis: 0.813 ± 0.047
2.443TyrIle: 2.443 ± 0.074
2.316TyrLys: 2.316 ± 0.073
3.632TyrLeu: 3.632 ± 0.098
0.56TyrMet: 0.56 ± 0.035
1.728TyrAsn: 1.728 ± 0.068
1.484TyrPro: 1.484 ± 0.064
1.878TyrGln: 1.878 ± 0.071
1.62TyrArg: 1.62 ± 0.067
2.114TyrSer: 2.114 ± 0.07
1.842TyrThr: 1.842 ± 0.069
1.804TyrVal: 1.804 ± 0.068
0.276TyrTrp: 0.276 ± 0.025
1.346TyrTyr: 1.346 ± 0.069
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1458 proteins (471126 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski