Caretta caretta papillomavirus 1
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6RUQ0|B6RUQ0_9PAPI E1 replication protein OS=Caretta caretta papillomavirus 1 OX=485241 GN=E1 PE=4 SV=1
MM1 pKa = 7.42 HH2 pKa = 7.45 NKK4 pKa = 9.04 GQPIKK9 pKa = 10.15 GYY11 pKa = 9.24 SGKK14 pKa = 10.64 YY15 pKa = 8.33 MCACCRR21 pKa = 11.84 KK22 pKa = 9.73 DD23 pKa = 3.42 VSFAQQEE30 pKa = 4.12 ICIMLSEE37 pKa = 4.06 QLGMLVCMNCEE48 pKa = 3.76 VVVPSNQIQDD58 pKa = 3.94 ALGTADD64 pKa = 3.83 LAEE67 pKa = 4.33 VTVSCGIGLVDD78 pKa = 4.69 EE79 pKa = 5.33 GSDD82 pKa = 3.25 LASYY86 pKa = 11.01 SEE88 pKa = 5.2 LEE90 pKa = 4.1 TDD92 pKa = 3.04 TDD94 pKa = 3.74 SDD96 pKa = 4.2 NEE98 pKa = 4.29 GEE100 pKa = 4.33 AFLAGTDD107 pKa = 3.78 SEE109 pKa = 4.8 AVV111 pKa = 3.23
Molecular weight: 11.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.851
IPC2_protein 3.872
IPC_protein 3.795
Toseland 3.605
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.528
Solomon 3.757
Lehninger 3.719
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 0.248
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|B6RUQ2|B6RUQ2_9PAPI Putative E4 protein OS=Caretta caretta papillomavirus 1 OX=485241 GN=E4 PE=4 SV=1
MM1 pKa = 8.04 DD2 pKa = 5.56 ANTRR6 pKa = 11.84 LMEE9 pKa = 4.28 MQDD12 pKa = 3.02 KK13 pKa = 10.73 QMTIIEE19 pKa = 4.88 KK20 pKa = 10.26 DD21 pKa = 3.66 DD22 pKa = 3.54 QSLDD26 pKa = 4.36 DD27 pKa = 3.43 ILEE30 pKa = 4.12 YY31 pKa = 11.13 YY32 pKa = 10.42 FAVKK36 pKa = 10.43 DD37 pKa = 3.86 EE38 pKa = 4.0 YY39 pKa = 11.33 LILAAARR46 pKa = 11.84 KK47 pKa = 9.93 AGVAHH52 pKa = 7.22 IGLQRR57 pKa = 11.84 VPSLQVSEE65 pKa = 4.36 SKK67 pKa = 10.81 YY68 pKa = 10.13 RR69 pKa = 11.84 EE70 pKa = 4.29 AVTMIIIVQSLQNSQFKK87 pKa = 10.33 NVNFTLRR94 pKa = 11.84 DD95 pKa = 3.47 LQYY98 pKa = 10.69 QLVMQAPAFTIKK110 pKa = 10.36 KK111 pKa = 9.3 GPKK114 pKa = 7.78 TVYY117 pKa = 8.84 VTYY120 pKa = 10.58 SGPDD124 pKa = 3.28 KK125 pKa = 10.8 LTVTHH130 pKa = 6.53 TKK132 pKa = 9.5 WKK134 pKa = 10.21 DD135 pKa = 2.66 IYY137 pKa = 9.5 YY138 pKa = 10.26 QRR140 pKa = 11.84 DD141 pKa = 3.65 EE142 pKa = 3.91 QWRR145 pKa = 11.84 SSKK148 pKa = 10.61 DD149 pKa = 3.34 LQHH152 pKa = 6.1 THH154 pKa = 6.85 DD155 pKa = 4.81 PNWFRR160 pKa = 11.84 AHH162 pKa = 6.56 TLTDD166 pKa = 3.7 SKK168 pKa = 11.63 GLFYY172 pKa = 11.11 VDD174 pKa = 3.51 IYY176 pKa = 11.41 GDD178 pKa = 2.95 VDD180 pKa = 4.16 YY181 pKa = 11.46 YY182 pKa = 11.86 VLFNSGEE189 pKa = 4.08 PQNATRR195 pKa = 11.84 KK196 pKa = 8.28 GQWEE200 pKa = 4.44 ISTSPPDD207 pKa = 3.45 ARR209 pKa = 11.84 TSTDD213 pKa = 3.0 TPSRR217 pKa = 11.84 KK218 pKa = 7.58 TPEE221 pKa = 3.63 RR222 pKa = 11.84 HH223 pKa = 5.37 IVAITPPIRR232 pKa = 11.84 NPRR235 pKa = 11.84 NPAYY239 pKa = 9.83 QSKK242 pKa = 8.26 PTHH245 pKa = 5.78 PTPTKK250 pKa = 8.27 PTHH253 pKa = 5.98 TSTVTTRR260 pKa = 11.84 RR261 pKa = 11.84 GNGRR265 pKa = 11.84 GRR267 pKa = 11.84 GLGGRR272 pKa = 11.84 GGGGGGAGGSTTRR285 pKa = 11.84 RR286 pKa = 11.84 PFAEE290 pKa = 4.03 KK291 pKa = 10.0 QSPVSAEE298 pKa = 3.79 EE299 pKa = 3.79 VGRR302 pKa = 11.84 TRR304 pKa = 11.84 EE305 pKa = 4.23 TVQGTGTRR313 pKa = 11.84 LEE315 pKa = 4.22 RR316 pKa = 11.84 LLKK319 pKa = 9.98 EE320 pKa = 4.38 ARR322 pKa = 11.84 DD323 pKa = 3.68 PPGLVFEE330 pKa = 5.3 GSTAQIKK337 pKa = 9.65 HH338 pKa = 4.21 IRR340 pKa = 11.84 RR341 pKa = 11.84 RR342 pKa = 11.84 VEE344 pKa = 3.94 AGSLKK349 pKa = 9.78 YY350 pKa = 10.82 SRR352 pKa = 11.84 VTSTWHH358 pKa = 6.04 WISGKK363 pKa = 10.21 KK364 pKa = 8.52 VLKK367 pKa = 7.84 PSKK370 pKa = 10.13 MIVVFNNEE378 pKa = 3.96 KK379 pKa = 10.28 EE380 pKa = 3.98 RR381 pKa = 11.84 STFLNLFRR389 pKa = 11.84 VEE391 pKa = 4.0 GDD393 pKa = 3.59 GITVRR398 pKa = 11.84 LCSFNGLL405 pKa = 3.61
Molecular weight: 45.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.109
IPC2_protein 9.209
IPC_protein 9.224
Toseland 9.897
ProMoST 9.604
Dawson 10.116
Bjellqvist 9.78
Wikipedia 10.292
Rodwell 10.482
Grimsley 10.189
Solomon 10.145
Lehninger 10.101
Nozaki 9.882
DTASelect 9.78
Thurlkill 9.955
EMBOSS 10.306
Sillero 10.028
Patrickios 9.736
IPC_peptide 10.145
IPC2_peptide 8.302
IPC2.peptide.svr19 8.227
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2254
85
561
322.0
35.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.61 ± 0.597
2.263 ± 0.713
6.034 ± 0.44
5.457 ± 0.459
4.525 ± 0.526
6.744 ± 0.664
2.085 ± 0.252
4.392 ± 0.414
5.324 ± 0.681
7.941 ± 0.682
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.307 ± 0.255
3.86 ± 0.477
7.72 ± 1.286
3.904 ± 0.488
5.723 ± 0.574
7.72 ± 0.637
6.832 ± 0.749
6.3 ± 0.709
1.287 ± 0.244
2.972 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here