Enterococcus canis
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2671 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L8RCN6|A0A1L8RCN6_9ENTE Methionine aminopeptidase OS=Enterococcus canis OX=214095 GN=map PE=3 SV=1
MM1 pKa = 7.16 TLAKK5 pKa = 9.5 IVYY8 pKa = 10.15 ASLTGNTEE16 pKa = 3.93 EE17 pKa = 4.62 IADD20 pKa = 3.64 IVAEE24 pKa = 4.06 AFEE27 pKa = 4.36 NLDD30 pKa = 3.66 LEE32 pKa = 4.7 VEE34 pKa = 4.34 INEE37 pKa = 4.35 CTQVEE42 pKa = 4.26 AEE44 pKa = 4.58 DD45 pKa = 5.45 FEE47 pKa = 5.56 DD48 pKa = 5.46 ADD50 pKa = 3.66 ICVVATYY57 pKa = 9.24 TYY59 pKa = 11.39 GDD61 pKa = 3.83 GEE63 pKa = 4.56 LPDD66 pKa = 4.96 EE67 pKa = 4.49 IVDD70 pKa = 4.49 FYY72 pKa = 11.88 EE73 pKa = 5.79 DD74 pKa = 3.69 LQEE77 pKa = 4.75 LNLAGKK83 pKa = 10.09 IYY85 pKa = 9.64 GVCGSGDD92 pKa = 3.21 TFYY95 pKa = 11.45 DD96 pKa = 4.02 EE97 pKa = 4.62 FCKK100 pKa = 10.96 SVDD103 pKa = 3.59 DD104 pKa = 4.43 FDD106 pKa = 5.9 AVFAKK111 pKa = 10.49 IGATKK116 pKa = 10.09 GAEE119 pKa = 4.18 SVKK122 pKa = 10.29 VDD124 pKa = 4.1 LAAEE128 pKa = 4.18 EE129 pKa = 4.06 EE130 pKa = 4.58 DD131 pKa = 4.27 IEE133 pKa = 4.39 NLEE136 pKa = 4.18 AFAKK140 pKa = 10.18 KK141 pKa = 9.73 LAAAAEE147 pKa = 4.24
Molecular weight: 15.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.605
IPC_protein 3.579
Toseland 3.376
ProMoST 3.706
Dawson 3.554
Bjellqvist 3.745
Wikipedia 3.465
Rodwell 3.401
Grimsley 3.287
Solomon 3.541
Lehninger 3.49
Nozaki 3.668
DTASelect 3.834
Thurlkill 3.427
EMBOSS 3.478
Sillero 3.694
Patrickios 0.947
IPC_peptide 3.541
IPC2_peptide 3.668
IPC2.peptide.svr19 3.677
Protein with the highest isoelectric point:
>tr|A0A1L8RF16|A0A1L8RF16_9ENTE Phosphotransferase system lactose/cellobiose-specific iib subunit OS=Enterococcus canis OX=214095 GN=RU97_GL001767 PE=4 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.12 QKK5 pKa = 7.54 THH7 pKa = 6.73 RR8 pKa = 11.84 GSAKK12 pKa = 9.57 RR13 pKa = 11.84 FKK15 pKa = 9.81 RR16 pKa = 11.84 TGGGGLKK23 pKa = 10.0 RR24 pKa = 11.84 GRR26 pKa = 11.84 AFTSHH31 pKa = 6.89 RR32 pKa = 11.84 FHH34 pKa = 7.48 GKK36 pKa = 6.37 TKK38 pKa = 9.57 KK39 pKa = 9.43 QRR41 pKa = 11.84 RR42 pKa = 11.84 QLRR45 pKa = 11.84 KK46 pKa = 9.82 ASMVSAGDD54 pKa = 3.61 FKK56 pKa = 11.12 RR57 pKa = 11.84 IRR59 pKa = 11.84 QQLSQMRR66 pKa = 3.75
Molecular weight: 7.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.446
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.501
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.223
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.058
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2671
0
2671
808566
37
2227
302.7
33.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.252 ± 0.058
0.553 ± 0.014
5.25 ± 0.046
7.098 ± 0.051
4.549 ± 0.04
6.818 ± 0.05
1.893 ± 0.02
6.991 ± 0.047
5.969 ± 0.046
10.394 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.68 ± 0.026
3.969 ± 0.036
3.761 ± 0.028
4.699 ± 0.045
4.076 ± 0.038
5.389 ± 0.041
6.021 ± 0.036
6.982 ± 0.037
1.036 ± 0.017
3.617 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here