Wuhan Fly Virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Domestica sigmavirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KXI3|A0A0B5KXI3_9RHAB X protein OS=Wuhan Fly Virus 2 OX=1608102 GN=X PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.55KK3 pKa = 9.18MEE5 pKa = 4.21TPRR8 pKa = 11.84GKK10 pKa = 9.96IRR12 pKa = 11.84GRR14 pKa = 11.84VNKK17 pKa = 9.2TRR19 pKa = 11.84VNNKK23 pKa = 9.15DD24 pKa = 3.24VCDD27 pKa = 3.69MLNNRR32 pKa = 11.84GGNLIKK38 pKa = 9.93TVEE41 pKa = 4.06NTLMRR46 pKa = 11.84DD47 pKa = 3.18NDD49 pKa = 3.68MMDD52 pKa = 3.25EE53 pKa = 4.25SEE55 pKa = 4.68APSTSGMNPNSVSDD69 pKa = 4.35PNDD72 pKa = 2.95IPLDD76 pKa = 3.51VLEE79 pKa = 4.62TGKK82 pKa = 10.59EE83 pKa = 3.83LLEE86 pKa = 4.3VVDD89 pKa = 4.13QEE91 pKa = 4.43EE92 pKa = 4.24DD93 pKa = 3.51SEE95 pKa = 4.6MEE97 pKa = 4.13CEE99 pKa = 4.36EE100 pKa = 4.16ILSVSEE106 pKa = 4.13VGGKK110 pKa = 9.97VYY112 pKa = 10.47FSVPPSKK119 pKa = 10.59VWTSEE124 pKa = 3.82QVVMFAAQFMEE135 pKa = 4.91IIKK138 pKa = 10.39GMKK141 pKa = 8.54SEE143 pKa = 5.42KK144 pKa = 8.9MDD146 pKa = 3.96CVPTPSAKK154 pKa = 9.75KK155 pKa = 10.26DD156 pKa = 3.5SPEE159 pKa = 3.99SKK161 pKa = 9.88KK162 pKa = 10.67PKK164 pKa = 8.62ITTPVPSTKK173 pKa = 9.98CDD175 pKa = 2.96ILKK178 pKa = 10.24RR179 pKa = 11.84LSEE182 pKa = 4.21GVVLKK187 pKa = 9.92PIKK190 pKa = 10.14IGLKK194 pKa = 9.47PIRR197 pKa = 11.84VTLDD201 pKa = 3.23NLGVSYY207 pKa = 11.09QEE209 pKa = 4.17AEE211 pKa = 4.44EE212 pKa = 3.91ILKK215 pKa = 10.5RR216 pKa = 11.84LEE218 pKa = 4.18DD219 pKa = 3.61ASIISLIRR227 pKa = 11.84SIKK230 pKa = 9.53ISKK233 pKa = 8.31GQNGRR238 pKa = 11.84FLSNYY243 pKa = 9.66KK244 pKa = 9.89EE245 pKa = 3.98

Molecular weight: 27.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KK73|A0A0B5KK73_9RHAB Glycoprotein OS=Wuhan Fly Virus 2 OX=1608102 GN=G PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 9.16KK3 pKa = 8.88TNTEE7 pKa = 3.41IKK9 pKa = 9.56TPPTSKK15 pKa = 10.34MNFNNFLSSFGKK27 pKa = 10.33QKK29 pKa = 10.77GSPEE33 pKa = 3.93KK34 pKa = 10.23RR35 pKa = 11.84DD36 pKa = 3.61LSVGLYY42 pKa = 10.27GPSAPFEE49 pKa = 4.67SISDD53 pKa = 3.7SLNVFEE59 pKa = 4.73YY60 pKa = 10.82TEE62 pKa = 4.37DD63 pKa = 3.59DD64 pKa = 3.96YY65 pKa = 11.94RR66 pKa = 11.84PLPEE70 pKa = 4.96TIPDD74 pKa = 3.56VSKK77 pKa = 8.23TWSVVGRR84 pKa = 11.84MEE86 pKa = 4.02YY87 pKa = 9.23LTNRR91 pKa = 11.84KK92 pKa = 8.88VSSWEE97 pKa = 3.96DD98 pKa = 3.32VLEE101 pKa = 4.56DD102 pKa = 4.26LDD104 pKa = 5.12VILDD108 pKa = 3.98EE109 pKa = 4.42YY110 pKa = 10.64RR111 pKa = 11.84GSVLYY116 pKa = 10.6RR117 pKa = 11.84EE118 pKa = 4.39IFIIFYY124 pKa = 10.49YY125 pKa = 11.11LMGTHH130 pKa = 6.98LRR132 pKa = 11.84PVQSPGGGLRR142 pKa = 11.84KK143 pKa = 9.52FRR145 pKa = 11.84IDD147 pKa = 2.99MEE149 pKa = 4.18ARR151 pKa = 11.84VNLVHH156 pKa = 7.31GFKK159 pKa = 10.63LDD161 pKa = 3.52NDD163 pKa = 3.94KK164 pKa = 11.11EE165 pKa = 4.25KK166 pKa = 10.93QINWTYY172 pKa = 11.92DD173 pKa = 2.94SMKK176 pKa = 10.31SIRR179 pKa = 11.84KK180 pKa = 8.81CRR182 pKa = 11.84ITYY185 pKa = 7.69MCSLKK190 pKa = 9.98DD191 pKa = 3.65TNRR194 pKa = 11.84KK195 pKa = 8.83GMPFKK200 pKa = 10.64TMYY203 pKa = 10.36LYY205 pKa = 10.23PSLEE209 pKa = 4.16GTPTPDD215 pKa = 4.15LKK217 pKa = 11.23KK218 pKa = 10.86LLDD221 pKa = 3.34PHH223 pKa = 7.01NIFFEE228 pKa = 4.49DD229 pKa = 3.35LGDD232 pKa = 3.41NYY234 pKa = 10.83RR235 pKa = 11.84FYY237 pKa = 11.31KK238 pKa = 10.44

Molecular weight: 27.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3938

238

2134

656.3

75.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.723 ± 0.427

1.549 ± 0.18

5.637 ± 0.279

6.374 ± 0.509

4.545 ± 0.272

5.053 ± 0.46

2.539 ± 0.442

6.475 ± 0.426

6.882 ± 0.412

9.751 ± 1.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.996 ± 0.381

5.079 ± 0.186

4.647 ± 0.398

2.996 ± 0.243

5.256 ± 0.142

7.923 ± 0.306

6.069 ± 0.338

6.094 ± 0.457

1.549 ± 0.246

3.86 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski