Aerococcus suis
Average proteome isoelectric point is 5.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1259 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W1Y2U8|A0A1W1Y2U8_9LACT Uncharacterized protein OS=Aerococcus suis OX=371602 GN=SAMN04487984_0191 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 IKK4 pKa = 10.72 YY5 pKa = 8.94 IRR7 pKa = 11.84 NVGFDD12 pKa = 3.37 FDD14 pKa = 3.74 IAQWIADD21 pKa = 4.06 SEE23 pKa = 4.48 LVSDD27 pKa = 5.58 DD28 pKa = 3.32 DD29 pKa = 3.75 CQYY32 pKa = 11.22 FMRR35 pKa = 11.84 YY36 pKa = 9.11 RR37 pKa = 11.84 DD38 pKa = 3.66 LDD40 pKa = 3.46 CWRR43 pKa = 11.84 VMVKK47 pKa = 10.16 QEE49 pKa = 3.9 DD50 pKa = 4.15 FAPEE54 pKa = 3.95 VYY56 pKa = 10.64 VIVIEE61 pKa = 4.39 GMNAYY66 pKa = 10.49 LIACEE71 pKa = 4.59 DD72 pKa = 3.77 DD73 pKa = 3.83 VLTEE77 pKa = 3.86 EE78 pKa = 4.44 TLQAFIRR85 pKa = 11.84 KK86 pKa = 6.99 TFDD89 pKa = 3.24 SIEE92 pKa = 3.98 YY93 pKa = 10.08 DD94 pKa = 3.65 CYY96 pKa = 11.37 DD97 pKa = 5.52 DD98 pKa = 5.41 EE99 pKa = 7.07 LSDD102 pKa = 3.86 TNSS105 pKa = 2.88
Molecular weight: 12.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.579
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.923
Patrickios 0.998
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A1W1ZC06|A0A1W1ZC06_9LACT Ribonuclease P protein component OS=Aerococcus suis OX=371602 GN=rnpA PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.7 QPKK8 pKa = 8.77 KK9 pKa = 8.07 RR10 pKa = 11.84 KK11 pKa = 8.85 RR12 pKa = 11.84 SKK14 pKa = 9.14 VHH16 pKa = 5.88 GFRR19 pKa = 11.84 KK20 pKa = 10.12 RR21 pKa = 11.84 MQTKK25 pKa = 9.26 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLKK32 pKa = 9.99 SRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.05 GRR39 pKa = 11.84 KK40 pKa = 8.81 VISRR44 pKa = 3.8
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.452
IPC2_protein 11.169
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.501
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.223
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.048
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1259
0
1259
398100
30
1912
316.2
35.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.373 ± 0.072
0.467 ± 0.016
6.434 ± 0.071
7.372 ± 0.079
4.103 ± 0.05
6.454 ± 0.076
2.286 ± 0.033
7.575 ± 0.074
5.99 ± 0.059
9.351 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.729 ± 0.032
4.774 ± 0.046
3.584 ± 0.032
4.63 ± 0.068
4.033 ± 0.063
5.732 ± 0.05
5.749 ± 0.047
6.76 ± 0.051
0.9 ± 0.023
3.704 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here