Jaminaea rosea
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6858 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316UKN2|A0A316UKN2_9BASI DUF1162-domain-containing protein OS=Jaminaea rosea OX=1569628 GN=BDZ90DRAFT_267720 PE=3 SV=1
MM1 pKa = 7.33 SPSFSSLFAFAALATTSLASSVTLQNSCSYY31 pKa = 9.28 PVWVRR36 pKa = 11.84 DD37 pKa = 3.7 SSPGANGRR45 pKa = 11.84 IVRR48 pKa = 11.84 VNANSPSTDD57 pKa = 3.03 VTQGGAGFVLNLLTGCSDD75 pKa = 4.25 DD76 pKa = 4.79 SGNGCATGDD85 pKa = 3.66 VGGGALPFGRR95 pKa = 11.84 VEE97 pKa = 4.04 ATFTDD102 pKa = 4.03 TYY104 pKa = 11.49 VNYY107 pKa = 10.29 DD108 pKa = 3.43 LSLEE112 pKa = 4.31 YY113 pKa = 10.57 GWNVPVEE120 pKa = 4.47 IGAPSCPYY128 pKa = 9.66 FACTSLPGCPVPGPDD143 pKa = 3.61 GSCYY147 pKa = 10.69 SPCCASADD155 pKa = 3.65 NCQGTNSCPYY165 pKa = 7.43 EE166 pKa = 4.06 TRR168 pKa = 11.84 DD169 pKa = 3.63 NGPGGKK175 pKa = 9.5 GVNSQYY181 pKa = 11.91 YY182 pKa = 9.4 MDD184 pKa = 4.42 TCPNAYY190 pKa = 9.48 AWPIEE195 pKa = 4.39 DD196 pKa = 3.74 SDD198 pKa = 4.31 VPSAYY203 pKa = 9.33 IDD205 pKa = 4.04 TNCGNQDD212 pKa = 3.5 LQINFCPPVGNSNIPRR228 pKa = 11.84 SS229 pKa = 3.65
Molecular weight: 23.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.875
IPC2_protein 3.732
IPC_protein 3.732
Toseland 3.503
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.139
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.859
Patrickios 0.006
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A316US67|A0A316US67_9BASI Pseudouridine synthase OS=Jaminaea rosea OX=1569628 GN=BDZ90DRAFT_55210 PE=3 SV=1
MM1 pKa = 7.65 NSALQDD7 pKa = 4.13 AIKK10 pKa = 10.7 NGQRR14 pKa = 11.84 LKK16 pKa = 10.91 KK17 pKa = 9.78 ATTNDD22 pKa = 2.88 RR23 pKa = 11.84 SAPAVAGNAGGGGGGGGGGGGGAGGIAAAAAAARR57 pKa = 11.84 TLASAPKK64 pKa = 10.21 VPGTGGGSGGGGAAGLGGILAGGIPKK90 pKa = 10.53 LKK92 pKa = 9.05 PTGAAPAAPKK102 pKa = 10.1 PPAASAPKK110 pKa = 9.86 PPAAPGRR117 pKa = 11.84 PVPPPTRR124 pKa = 11.84 PSAPPAPPPPSRR136 pKa = 11.84 PSAAAPPPPPPPGRR150 pKa = 11.84 PAAPSAAAPPRR161 pKa = 11.84 PPPPPPGRR169 pKa = 11.84 SSPAVPPPPPPPARR183 pKa = 11.84 PAAASPAAPAPPPPPPPGRR202 pKa = 11.84 PAAAAPPPPSRR213 pKa = 11.84 PSAAAPPPPPPPPAASSSAPARR235 pKa = 11.84 GVPPPPSRR243 pKa = 11.84 APAPPSRR250 pKa = 11.84 PGAAPPAPPPPSTPARR266 pKa = 11.84 GAVPPPPARR275 pKa = 11.84 ASPISSAPPPPPSRR289 pKa = 11.84 PASSAPPPPPPTRR302 pKa = 11.84 SAAAAPAAPPPPPPSRR318 pKa = 11.84 APAAAPPAPPPSRR331 pKa = 11.84 APAAAPPTPPPSRR344 pKa = 11.84 APAAPPPSRR353 pKa = 11.84 SAAAAALPALNGAGSNGASRR373 pKa = 11.84 SVSSTSISGGAAGKK387 pKa = 8.23 TGRR390 pKa = 11.84 IPSPPPTSGNLTFSPSSAFPPPRR413 pKa = 11.84 AFAGSGASRR422 pKa = 11.84 VYY424 pKa = 10.53 PGGKK428 pKa = 7.54 TRR430 pKa = 11.84 GSTFDD435 pKa = 3.05 WSTVGG440 pKa = 4.33
Molecular weight: 40.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.428
IPC2_protein 10.994
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.325
Grimsley 12.705
Solomon 13.159
Lehninger 13.071
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.173
Sillero 12.661
Patrickios 12.032
IPC_peptide 13.173
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6858
0
6858
3700262
50
4870
539.6
58.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.072 ± 0.033
1.022 ± 0.011
5.454 ± 0.022
6.068 ± 0.026
2.918 ± 0.019
8.036 ± 0.032
2.358 ± 0.013
3.518 ± 0.021
4.175 ± 0.025
8.696 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.897 ± 0.009
2.719 ± 0.013
6.42 ± 0.034
4.162 ± 0.018
6.923 ± 0.023
9.933 ± 0.048
5.588 ± 0.018
5.663 ± 0.02
1.292 ± 0.01
2.087 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here