Escherichia phage EP335

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kuravirus; unclassified Kuravirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z3DRN9|A0A2Z3DRN9_9CAUD Putative capsid and scaffold protein OS=Escherichia phage EP335 OX=2070199 PE=4 SV=1
MM1 pKa = 7.3GNEE4 pKa = 4.34TIALIDD10 pKa = 3.28THH12 pKa = 7.57NYY14 pKa = 10.11GGVTLSFDD22 pKa = 3.4EE23 pKa = 4.75SSDD26 pKa = 3.5VGYY29 pKa = 11.06LSFLSKK35 pKa = 11.5GEE37 pKa = 3.78IQTIRR42 pKa = 11.84FPVVCLVQDD51 pKa = 3.63YY52 pKa = 8.14LTVGKK57 pKa = 10.27YY58 pKa = 9.13LAQRR62 pKa = 11.84GILLSLEE69 pKa = 4.07EE70 pKa = 4.31VEE72 pKa = 4.68QISISFFGEE81 pKa = 4.06DD82 pKa = 4.47GNDD85 pKa = 3.53DD86 pKa = 3.67SS87 pKa = 5.54

Molecular weight: 9.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z3DJF9|A0A2Z3DJF9_9CAUD Uncharacterized protein OS=Escherichia phage EP335 OX=2070199 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.5KK3 pKa = 8.54STKK6 pKa = 10.17RR7 pKa = 11.84MNRR10 pKa = 11.84PEE12 pKa = 4.44RR13 pKa = 11.84MDD15 pKa = 3.65AVANGALKK23 pKa = 10.33IEE25 pKa = 4.35SAMLRR30 pKa = 11.84LTGNIRR36 pKa = 11.84KK37 pKa = 8.79RR38 pKa = 11.84QGDD41 pKa = 3.3WSYY44 pKa = 11.98GGG46 pKa = 3.56

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

125

0

125

22842

28

1473

182.7

20.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.863 ± 0.56

1.173 ± 0.151

6.147 ± 0.214

6.952 ± 0.312

3.818 ± 0.159

7.132 ± 0.316

1.751 ± 0.137

5.595 ± 0.22

6.239 ± 0.298

7.39 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.762 ± 0.117

5.538 ± 0.239

3.686 ± 0.155

4.093 ± 0.315

5.183 ± 0.253

6.926 ± 0.232

5.809 ± 0.333

7.083 ± 0.279

1.467 ± 0.106

3.393 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski