Amino acid dipepetide frequency for Paucibacter sp. KBW04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.223AlaAla: 18.223 ± 0.186
1.349AlaCys: 1.349 ± 0.028
5.961AlaAsp: 5.961 ± 0.06
7.532AlaGlu: 7.532 ± 0.08
3.892AlaPhe: 3.892 ± 0.046
10.069AlaGly: 10.069 ± 0.086
2.479AlaHis: 2.479 ± 0.041
4.689AlaIle: 4.689 ± 0.062
4.682AlaLys: 4.682 ± 0.068
16.635AlaLeu: 16.635 ± 0.202
3.322AlaMet: 3.322 ± 0.049
3.161AlaAsn: 3.161 ± 0.049
5.841AlaPro: 5.841 ± 0.078
7.529AlaGln: 7.529 ± 0.087
8.157AlaArg: 8.157 ± 0.083
7.765AlaSer: 7.765 ± 0.101
5.163AlaThr: 5.163 ± 0.06
7.894AlaVal: 7.894 ± 0.081
2.241AlaTrp: 2.241 ± 0.04
2.482AlaTyr: 2.482 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
1.169CysAla: 1.169 ± 0.029
0.128CysCys: 0.128 ± 0.01
0.464CysAsp: 0.464 ± 0.019
0.547CysGlu: 0.547 ± 0.021
0.328CysPhe: 0.328 ± 0.014
0.896CysGly: 0.896 ± 0.025
0.227CysHis: 0.227 ± 0.011
0.351CysIle: 0.351 ± 0.016
0.257CysLys: 0.257 ± 0.013
1.03CysLeu: 1.03 ± 0.028
0.199CysMet: 0.199 ± 0.012
0.215CysAsn: 0.215 ± 0.011
0.483CysPro: 0.483 ± 0.018
0.353CysGln: 0.353 ± 0.013
0.526CysArg: 0.526 ± 0.019
0.589CysSer: 0.589 ± 0.021
0.438CysThr: 0.438 ± 0.016
0.619CysVal: 0.619 ± 0.018
0.141CysTrp: 0.141 ± 0.01
0.198CysTyr: 0.198 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
6.272AspAla: 6.272 ± 0.068
0.457AspCys: 0.457 ± 0.016
2.095AspAsp: 2.095 ± 0.052
3.129AspGlu: 3.129 ± 0.048
2.022AspPhe: 2.022 ± 0.04
4.182AspGly: 4.182 ± 0.068
0.918AspHis: 0.918 ± 0.023
2.023AspIle: 2.023 ± 0.04
1.81AspLys: 1.81 ± 0.036
5.793AspLeu: 5.793 ± 0.062
1.009AspMet: 1.009 ± 0.022
1.081AspAsn: 1.081 ± 0.028
2.608AspPro: 2.608 ± 0.043
1.861AspGln: 1.861 ± 0.041
2.757AspArg: 2.757 ± 0.045
2.53AspSer: 2.53 ± 0.048
1.939AspThr: 1.939 ± 0.043
3.28AspVal: 3.28 ± 0.047
1.082AspTrp: 1.082 ± 0.026
1.361AspTyr: 1.361 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.701GluAla: 7.701 ± 0.086
0.371GluCys: 0.371 ± 0.017
2.27GluAsp: 2.27 ± 0.038
2.547GluGlu: 2.547 ± 0.049
1.87GluPhe: 1.87 ± 0.033
3.993GluGly: 3.993 ± 0.058
1.275GluHis: 1.275 ± 0.028
2.622GluIle: 2.622 ± 0.042
1.761GluLys: 1.761 ± 0.04
7.044GluLeu: 7.044 ± 0.075
1.284GluMet: 1.284 ± 0.03
1.197GluAsn: 1.197 ± 0.029
2.534GluPro: 2.534 ± 0.047
3.27GluGln: 3.27 ± 0.048
4.5GluArg: 4.5 ± 0.074
2.705GluSer: 2.705 ± 0.047
2.28GluThr: 2.28 ± 0.039
3.959GluVal: 3.959 ± 0.056
0.713GluTrp: 0.713 ± 0.02
0.939GluTyr: 0.939 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.197PheAla: 4.197 ± 0.051
0.412PheCys: 0.412 ± 0.017
2.353PheAsp: 2.353 ± 0.041
2.297PheGlu: 2.297 ± 0.042
1.442PhePhe: 1.442 ± 0.035
3.245PheGly: 3.245 ± 0.053
0.675PheHis: 0.675 ± 0.022
1.403PheIle: 1.403 ± 0.029
1.438PheLys: 1.438 ± 0.03
2.955PheLeu: 2.955 ± 0.049
0.733PheMet: 0.733 ± 0.022
1.196PheAsn: 1.196 ± 0.027
1.319PhePro: 1.319 ± 0.03
1.15PheGln: 1.15 ± 0.026
1.665PheArg: 1.665 ± 0.037
2.441PheSer: 2.441 ± 0.039
1.634PheThr: 1.634 ± 0.034
2.624PheVal: 2.624 ± 0.045
0.566PheTrp: 0.566 ± 0.021
0.89PheTyr: 0.89 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
8.634GlyAla: 8.634 ± 0.086
0.828GlyCys: 0.828 ± 0.024
3.647GlyAsp: 3.647 ± 0.056
4.617GlyGlu: 4.617 ± 0.054
3.332GlyPhe: 3.332 ± 0.047
6.783GlyGly: 6.783 ± 0.115
1.772GlyHis: 1.772 ± 0.034
3.275GlyIle: 3.275 ± 0.05
3.193GlyLys: 3.193 ± 0.052
10.841GlyLeu: 10.841 ± 0.096
2.047GlyMet: 2.047 ± 0.035
2.21GlyAsn: 2.21 ± 0.071
3.115GlyPro: 3.115 ± 0.051
4.257GlyGln: 4.257 ± 0.052
5.392GlyArg: 5.392 ± 0.075
5.018GlySer: 5.018 ± 0.087
3.576GlyThr: 3.576 ± 0.071
5.722GlyVal: 5.722 ± 0.064
1.371GlyTrp: 1.371 ± 0.037
2.083GlyTyr: 2.083 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.544HisAla: 2.544 ± 0.047
0.289HisCys: 0.289 ± 0.015
0.932HisAsp: 0.932 ± 0.025
1.209HisGlu: 1.209 ± 0.027
0.831HisPhe: 0.831 ± 0.023
1.87HisGly: 1.87 ± 0.038
0.553HisHis: 0.553 ± 0.021
0.859HisIle: 0.859 ± 0.022
0.608HisLys: 0.608 ± 0.019
2.406HisLeu: 2.406 ± 0.039
0.402HisMet: 0.402 ± 0.016
0.432HisAsn: 0.432 ± 0.018
1.273HisPro: 1.273 ± 0.031
0.827HisGln: 0.827 ± 0.023
1.302HisArg: 1.302 ± 0.032
1.2HisSer: 1.2 ± 0.024
0.834HisThr: 0.834 ± 0.022
1.266HisVal: 1.266 ± 0.028
0.406HisTrp: 0.406 ± 0.016
0.581HisTyr: 0.581 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
5.527IleAla: 5.527 ± 0.067
0.438IleCys: 0.438 ± 0.016
2.482IleAsp: 2.482 ± 0.039
2.873IleGlu: 2.873 ± 0.058
1.249IlePhe: 1.249 ± 0.032
3.437IleGly: 3.437 ± 0.052
0.802IleHis: 0.802 ± 0.021
1.422IleIle: 1.422 ± 0.037
1.764IleLys: 1.764 ± 0.035
3.268IleLeu: 3.268 ± 0.05
0.669IleMet: 0.669 ± 0.018
1.518IleAsn: 1.518 ± 0.03
1.796IlePro: 1.796 ± 0.031
1.507IleGln: 1.507 ± 0.036
2.267IleArg: 2.267 ± 0.045
2.829IleSer: 2.829 ± 0.05
2.157IleThr: 2.157 ± 0.043
2.837IleVal: 2.837 ± 0.047
0.497IleTrp: 0.497 ± 0.02
0.947IleTyr: 0.947 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
4.44LysAla: 4.44 ± 0.072
0.151LysCys: 0.151 ± 0.012
1.707LysAsp: 1.707 ± 0.037
1.496LysGlu: 1.496 ± 0.033
1.057LysPhe: 1.057 ± 0.031
2.473LysGly: 2.473 ± 0.048
0.724LysHis: 0.724 ± 0.02
1.525LysIle: 1.525 ± 0.033
1.487LysLys: 1.487 ± 0.046
4.058LysLeu: 4.058 ± 0.056
0.733LysMet: 0.733 ± 0.023
1.056LysAsn: 1.056 ± 0.027
2.557LysPro: 2.557 ± 0.045
1.574LysGln: 1.574 ± 0.03
2.34LysArg: 2.34 ± 0.039
2.089LysSer: 2.089 ± 0.043
2.075LysThr: 2.075 ± 0.04
2.3LysVal: 2.3 ± 0.048
0.315LysTrp: 0.315 ± 0.013
0.621LysTyr: 0.621 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
16.283LeuAla: 16.283 ± 0.16
1.084LeuCys: 1.084 ± 0.029
6.162LeuAsp: 6.162 ± 0.079
5.418LeuGlu: 5.418 ± 0.075
3.459LeuPhe: 3.459 ± 0.053
9.969LeuGly: 9.969 ± 0.089
2.342LeuHis: 2.342 ± 0.042
4.796LeuIle: 4.796 ± 0.061
4.166LeuLys: 4.166 ± 0.058
13.833LeuLeu: 13.833 ± 0.151
2.826LeuMet: 2.826 ± 0.047
3.403LeuAsn: 3.403 ± 0.062
6.643LeuPro: 6.643 ± 0.071
5.855LeuGln: 5.855 ± 0.08
8.778LeuArg: 8.778 ± 0.092
8.619LeuSer: 8.619 ± 0.123
4.978LeuThr: 4.978 ± 0.068
7.288LeuVal: 7.288 ± 0.067
1.545LeuTrp: 1.545 ± 0.035
1.989LeuTyr: 1.989 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
2.887MetAla: 2.887 ± 0.045
0.153MetCys: 0.153 ± 0.01
1.085MetAsp: 1.085 ± 0.028
1.045MetGlu: 1.045 ± 0.021
0.703MetPhe: 0.703 ± 0.022
1.852MetGly: 1.852 ± 0.037
0.445MetHis: 0.445 ± 0.016
0.826MetIle: 0.826 ± 0.022
0.961MetLys: 0.961 ± 0.025
2.638MetLeu: 2.638 ± 0.049
0.557MetMet: 0.557 ± 0.018
0.835MetAsn: 0.835 ± 0.023
1.416MetPro: 1.416 ± 0.033
1.157MetGln: 1.157 ± 0.026
1.61MetArg: 1.61 ± 0.033
1.826MetSer: 1.826 ± 0.031
1.221MetThr: 1.221 ± 0.03
1.546MetVal: 1.546 ± 0.032
0.206MetTrp: 0.206 ± 0.011
0.306MetTyr: 0.306 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.313AsnAla: 3.313 ± 0.048
0.257AsnCys: 0.257 ± 0.012
1.227AsnAsp: 1.227 ± 0.048
1.417AsnGlu: 1.417 ± 0.029
1.076AsnPhe: 1.076 ± 0.03
2.227AsnGly: 2.227 ± 0.055
0.524AsnHis: 0.524 ± 0.019
1.195AsnIle: 1.195 ± 0.026
0.989AsnLys: 0.989 ± 0.028
2.923AsnLeu: 2.923 ± 0.041
0.556AsnMet: 0.556 ± 0.018
0.838AsnAsn: 0.838 ± 0.027
1.773AsnPro: 1.773 ± 0.032
1.091AsnGln: 1.091 ± 0.033
1.543AsnArg: 1.543 ± 0.032
1.714AsnSer: 1.714 ± 0.052
1.443AsnThr: 1.443 ± 0.039
1.698AsnVal: 1.698 ± 0.037
0.456AsnTrp: 0.456 ± 0.017
0.739AsnTyr: 0.739 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.926ProAla: 6.926 ± 0.073
0.377ProCys: 0.377 ± 0.015
2.554ProAsp: 2.554 ± 0.039
3.538ProGlu: 3.538 ± 0.047
1.638ProPhe: 1.638 ± 0.03
4.402ProGly: 4.402 ± 0.057
0.935ProHis: 0.935 ± 0.023
1.758ProIle: 1.758 ± 0.036
1.806ProLys: 1.806 ± 0.036
5.604ProLeu: 5.604 ± 0.073
1.263ProMet: 1.263 ± 0.032
1.288ProAsn: 1.288 ± 0.028
2.393ProPro: 2.393 ± 0.043
2.498ProGln: 2.498 ± 0.042
2.798ProArg: 2.798 ± 0.051
3.2ProSer: 3.2 ± 0.053
2.266ProThr: 2.266 ± 0.036
3.631ProVal: 3.631 ± 0.053
0.797ProTrp: 0.797 ± 0.025
1.011ProTyr: 1.011 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
8.091GlnAla: 8.091 ± 0.101
0.32GlnCys: 0.32 ± 0.014
2.092GlnAsp: 2.092 ± 0.037
2.071GlnGlu: 2.071 ± 0.041
1.443GlnPhe: 1.443 ± 0.026
4.088GlnGly: 4.088 ± 0.055
1.094GlnHis: 1.094 ± 0.028
2.183GlnIle: 2.183 ± 0.064
1.194GlnLys: 1.194 ± 0.028
5.486GlnLeu: 5.486 ± 0.072
1.117GlnMet: 1.117 ± 0.03
0.988GlnAsn: 0.988 ± 0.023
2.428GlnPro: 2.428 ± 0.04
2.489GlnGln: 2.489 ± 0.05
3.964GlnArg: 3.964 ± 0.055
2.561GlnSer: 2.561 ± 0.04
2.326GlnThr: 2.326 ± 0.043
3.469GlnVal: 3.469 ± 0.084
0.733GlnTrp: 0.733 ± 0.022
0.794GlnTyr: 0.794 ± 0.029
0.001GlnXaa: 0.001 ± 0.001
Arg
7.488ArgAla: 7.488 ± 0.083
0.631ArgCys: 0.631 ± 0.023
3.09ArgAsp: 3.09 ± 0.048
4.214ArgGlu: 4.214 ± 0.063
2.634ArgPhe: 2.634 ± 0.043
4.48ArgGly: 4.48 ± 0.06
1.545ArgHis: 1.545 ± 0.034
2.989ArgIle: 2.989 ± 0.045
1.896ArgLys: 1.896 ± 0.039
8.474ArgLeu: 8.474 ± 0.093
1.728ArgMet: 1.728 ± 0.032
1.733ArgAsn: 1.733 ± 0.034
3.084ArgPro: 3.084 ± 0.049
3.366ArgGln: 3.366 ± 0.063
4.666ArgArg: 4.666 ± 0.069
3.883ArgSer: 3.883 ± 0.045
2.297ArgThr: 2.297 ± 0.039
4.688ArgVal: 4.688 ± 0.056
1.195ArgTrp: 1.195 ± 0.029
1.79ArgTyr: 1.79 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
7.477SerAla: 7.477 ± 0.084
0.56SerCys: 0.56 ± 0.019
2.804SerAsp: 2.804 ± 0.066
3.117SerGlu: 3.117 ± 0.048
2.447SerPhe: 2.447 ± 0.045
5.698SerGly: 5.698 ± 0.11
1.292SerHis: 1.292 ± 0.03
2.422SerIle: 2.422 ± 0.045
2.095SerLys: 2.095 ± 0.039
7.414SerLeu: 7.414 ± 0.089
1.37SerMet: 1.37 ± 0.03
1.757SerAsn: 1.757 ± 0.046
3.299SerPro: 3.299 ± 0.052
2.813SerGln: 2.813 ± 0.063
3.745SerArg: 3.745 ± 0.047
4.308SerSer: 4.308 ± 0.089
3.05SerThr: 3.05 ± 0.08
3.914SerVal: 3.914 ± 0.056
0.945SerTrp: 0.945 ± 0.021
1.539SerTyr: 1.539 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
5.436ThrAla: 5.436 ± 0.066
0.332ThrCys: 0.332 ± 0.014
1.909ThrAsp: 1.909 ± 0.05
2.25ThrGlu: 2.25 ± 0.04
1.311ThrPhe: 1.311 ± 0.032
3.982ThrGly: 3.982 ± 0.058
0.956ThrHis: 0.956 ± 0.027
1.631ThrIle: 1.631 ± 0.037
1.155ThrLys: 1.155 ± 0.034
5.905ThrLeu: 5.905 ± 0.056
0.912ThrMet: 0.912 ± 0.023
1.079ThrAsn: 1.079 ± 0.029
3.141ThrPro: 3.141 ± 0.049
2.21ThrGln: 2.21 ± 0.037
2.758ThrArg: 2.758 ± 0.042
2.54ThrSer: 2.54 ± 0.056
2.2ThrThr: 2.2 ± 0.055
3.618ThrVal: 3.618 ± 0.059
0.618ThrTrp: 0.618 ± 0.022
0.878ThrTyr: 0.878 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
8.146ValAla: 8.146 ± 0.072
0.657ValCys: 0.657 ± 0.023
3.689ValAsp: 3.689 ± 0.049
3.862ValGlu: 3.862 ± 0.054
2.484ValPhe: 2.484 ± 0.039
5.126ValGly: 5.126 ± 0.061
1.34ValHis: 1.34 ± 0.028
2.958ValIle: 2.958 ± 0.051
2.38ValLys: 2.38 ± 0.048
8.397ValLeu: 8.397 ± 0.085
1.712ValMet: 1.712 ± 0.035
1.995ValAsn: 1.995 ± 0.04
3.163ValPro: 3.163 ± 0.049
3.171ValGln: 3.171 ± 0.053
4.16ValArg: 4.16 ± 0.054
4.127ValSer: 4.127 ± 0.054
3.082ValThr: 3.082 ± 0.056
4.904ValVal: 4.904 ± 0.068
0.984ValTrp: 0.984 ± 0.025
1.427ValTyr: 1.427 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.711TrpAla: 1.711 ± 0.041
0.148TrpCys: 0.148 ± 0.01
0.641TrpAsp: 0.641 ± 0.019
0.53TrpGlu: 0.53 ± 0.019
0.515TrpPhe: 0.515 ± 0.018
1.061TrpGly: 1.061 ± 0.028
0.35TrpHis: 0.35 ± 0.015
0.593TrpIle: 0.593 ± 0.019
0.42TrpLys: 0.42 ± 0.016
2.293TrpLeu: 2.293 ± 0.046
0.454TrpMet: 0.454 ± 0.015
0.398TrpAsn: 0.398 ± 0.015
0.829TrpPro: 0.829 ± 0.024
0.967TrpGln: 0.967 ± 0.022
1.342TrpArg: 1.342 ± 0.029
0.832TrpSer: 0.832 ± 0.026
0.757TrpThr: 0.757 ± 0.023
1.086TrpVal: 1.086 ± 0.03
0.285TrpTrp: 0.285 ± 0.013
0.274TrpTyr: 0.274 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.597TyrAla: 2.597 ± 0.046
0.213TyrCys: 0.213 ± 0.012
1.049TyrAsp: 1.049 ± 0.058
1.124TyrGlu: 1.124 ± 0.028
0.899TyrPhe: 0.899 ± 0.023
1.881TyrGly: 1.881 ± 0.034
0.376TyrHis: 0.376 ± 0.015
0.765TyrIle: 0.765 ± 0.02
0.714TyrLys: 0.714 ± 0.023
2.44TyrLeu: 2.44 ± 0.035
0.383TyrMet: 0.383 ± 0.016
0.62TyrAsn: 0.62 ± 0.025
0.987TyrPro: 0.987 ± 0.028
0.99TyrGln: 0.99 ± 0.023
1.604TyrArg: 1.604 ± 0.033
1.289TyrSer: 1.289 ± 0.036
1.106TyrThr: 1.106 ± 0.029
1.424TyrVal: 1.424 ± 0.032
0.389TyrTrp: 0.389 ± 0.014
0.511TyrTyr: 0.511 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.001XaaGly: 0.001 ± 0.001
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4784 proteins (1670315 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski