Colletotrichum fructicola chrysovirus 1
Average proteome isoelectric point is 7.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346IME2|A0A346IME2_9VIRU Uncharacterized protein OS=Colletotrichum fructicola chrysovirus 1 OX=2304034 PE=4 SV=1
MM1 pKa = 6.59 STPYY5 pKa = 10.42 FGVNIDD11 pKa = 3.56 ARR13 pKa = 11.84 LMEE16 pKa = 5.71 LDD18 pKa = 3.56 DD19 pKa = 4.4 AYY21 pKa = 10.24 KK22 pKa = 10.23 FRR24 pKa = 11.84 RR25 pKa = 11.84 ISARR29 pKa = 11.84 QLIEE33 pKa = 3.96 HH34 pKa = 6.26 VEE36 pKa = 3.91 PLLPLLEE43 pKa = 4.64 GPVPQEE49 pKa = 3.7 TRR51 pKa = 11.84 DD52 pKa = 3.58 SVRR55 pKa = 11.84 NAEE58 pKa = 4.13 RR59 pKa = 11.84 EE60 pKa = 4.11 LGEE63 pKa = 3.92 VTRR66 pKa = 11.84 LHH68 pKa = 6.25 EE69 pKa = 5.25 CGLGTADD76 pKa = 4.38 CATEE80 pKa = 3.89 RR81 pKa = 11.84 GGGEE85 pKa = 3.94 EE86 pKa = 4.31 LVVEE90 pKa = 4.53 VARR93 pKa = 11.84 VYY95 pKa = 11.36 SDD97 pKa = 3.3 AAAAATKK104 pKa = 9.86 HH105 pKa = 6.41 HH106 pKa = 6.71 NEE108 pKa = 3.5 QSEE111 pKa = 3.99 WGRR114 pKa = 11.84 NRR116 pKa = 11.84 DD117 pKa = 3.19 TRR119 pKa = 11.84 IMRR122 pKa = 11.84 RR123 pKa = 11.84 GHH125 pKa = 6.77 HH126 pKa = 7.05 DD127 pKa = 3.14 VTPINRR133 pKa = 11.84 NLISMSYY140 pKa = 10.06 GLPKK144 pKa = 10.53 GADD147 pKa = 3.24 VRR149 pKa = 11.84 ALAWTDD155 pKa = 3.1 ADD157 pKa = 3.6 VSRR160 pKa = 11.84 PRR162 pKa = 11.84 LLNTLVAGASSLHH175 pKa = 5.91 KK176 pKa = 10.29 LWRR179 pKa = 11.84 LQACSVTLPSGRR191 pKa = 11.84 VLMSEE196 pKa = 4.93 AYY198 pKa = 9.17 PGRR201 pKa = 11.84 GLGGMDD207 pKa = 2.83 MYY209 pKa = 11.24 GFNLHH214 pKa = 6.36 EE215 pKa = 4.31 VALYY219 pKa = 8.27 LTISEE224 pKa = 4.76 DD225 pKa = 3.61 LLVDD229 pKa = 3.37 VSVAGKK235 pKa = 9.82 PEE237 pKa = 3.8 GLRR240 pKa = 11.84 VTNRR244 pKa = 11.84 EE245 pKa = 3.94 RR246 pKa = 11.84 FDD248 pKa = 3.76 EE249 pKa = 4.91 FLDD252 pKa = 3.83 LCSIVQFWIVDD263 pKa = 3.39 RR264 pKa = 11.84 MVPISGLNVLEE275 pKa = 5.2 GYY277 pKa = 8.2 TLAQLKK283 pKa = 8.45 TLTTRR288 pKa = 11.84 TQQACTPSGHH298 pKa = 5.62 MVRR301 pKa = 11.84 GPIGALVSLGEE312 pKa = 4.1 WEE314 pKa = 4.76 ADD316 pKa = 3.37 DD317 pKa = 4.11 SEE319 pKa = 5.52 KK320 pKa = 10.76 IGVSHH325 pKa = 7.05 CAADD329 pKa = 3.98 GTVSRR334 pKa = 11.84 GTLSPNNIPPGLLTVYY350 pKa = 8.78 ATSGGKK356 pKa = 7.59 TVTGGTLTLRR366 pKa = 11.84 GEE368 pKa = 4.23 EE369 pKa = 4.07 GVYY372 pKa = 9.73 TVTSSVTANMTIGHH386 pKa = 6.35 IKK388 pKa = 9.92 EE389 pKa = 4.21 LSKK392 pKa = 10.84 CAPTVRR398 pKa = 11.84 DD399 pKa = 3.62 GLFSPTAAGALLTDD413 pKa = 4.22 GDD415 pKa = 3.86 GGIYY419 pKa = 10.15 VDD421 pKa = 4.18 VNHH424 pKa = 6.28 EE425 pKa = 4.24 HH426 pKa = 6.26 VVVPTIFSGVPDD438 pKa = 3.92 GSEE441 pKa = 3.69 IEE443 pKa = 4.46 VVARR447 pKa = 11.84 NCSAMLGYY455 pKa = 10.64 LEE457 pKa = 5.86 DD458 pKa = 3.69 VAALQGWSYY467 pKa = 11.3 SVEE470 pKa = 3.94 LGGTQFNIDD479 pKa = 3.87 PSEE482 pKa = 4.42 DD483 pKa = 3.73 GAWARR488 pKa = 11.84 IVVKK492 pKa = 10.34 IRR494 pKa = 11.84 YY495 pKa = 8.28 GPEE498 pKa = 3.54 VRR500 pKa = 11.84 PQWATIVAGEE510 pKa = 4.14 LDD512 pKa = 3.95 EE513 pKa = 5.32 LAVDD517 pKa = 3.59 SEE519 pKa = 4.21 LRR521 pKa = 11.84 LDD523 pKa = 3.83 YY524 pKa = 10.86 GVSGLGRR531 pKa = 11.84 YY532 pKa = 8.64 LLSRR536 pKa = 11.84 FLVGGTFIPGTSLKK550 pKa = 10.07 INKK553 pKa = 9.3 RR554 pKa = 11.84 GRR556 pKa = 11.84 FGAIHH561 pKa = 6.75 GVLKK565 pKa = 10.29 EE566 pKa = 4.03 NKK568 pKa = 8.98 AALMLTGSLPTPTNYY583 pKa = 9.71 VVDD586 pKa = 4.24 VKK588 pKa = 10.36 TLARR592 pKa = 11.84 TRR594 pKa = 11.84 GSEE597 pKa = 3.99 TQYY600 pKa = 9.26 YY601 pKa = 10.0 AYY603 pKa = 10.27 AIGVAKK609 pKa = 9.71 FIRR612 pKa = 11.84 NQYY615 pKa = 9.16 VGSACCVDD623 pKa = 4.93 DD624 pKa = 5.62 SPEE627 pKa = 4.02 LDD629 pKa = 3.55 EE630 pKa = 5.44 FLSANRR636 pKa = 11.84 DD637 pKa = 3.66 EE638 pKa = 5.82 LPDD641 pKa = 3.58 EE642 pKa = 4.71 VGALLANPEE651 pKa = 4.21 VPSRR655 pKa = 11.84 DD656 pKa = 3.27 VTTVLSEE663 pKa = 3.97 LRR665 pKa = 11.84 ALEE668 pKa = 4.26 SPPTCVCSLRR678 pKa = 11.84 DD679 pKa = 3.34 VDD681 pKa = 5.37 TSTQCYY687 pKa = 7.94 MEE689 pKa = 4.77 SMSAGPVYY697 pKa = 10.69 SGGRR701 pKa = 11.84 IITVLYY707 pKa = 10.63
Molecular weight: 76.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.007
IPC2_protein 5.067
IPC_protein 5.029
Toseland 4.978
ProMoST 5.169
Dawson 5.029
Bjellqvist 5.156
Wikipedia 4.902
Rodwell 4.94
Grimsley 4.902
Solomon 5.029
Lehninger 4.991
Nozaki 5.156
DTASelect 5.296
Thurlkill 4.978
EMBOSS 4.94
Sillero 5.219
Patrickios 3.884
IPC_peptide 5.041
IPC2_peptide 5.219
IPC2.peptide.svr19 5.158
Protein with the highest isoelectric point:
>tr|A0A346IME4|A0A346IME4_9VIRU Uncharacterized protein OS=Colletotrichum fructicola chrysovirus 1 OX=2304034 PE=4 SV=1
MM1 pKa = 8.12 DD2 pKa = 4.38 SSRR5 pKa = 11.84 DD6 pKa = 3.52 KK7 pKa = 11.37 KK8 pKa = 10.78 EE9 pKa = 3.6 AGQRR13 pKa = 11.84 RR14 pKa = 11.84 LVSARR19 pKa = 11.84 WRR21 pKa = 11.84 VRR23 pKa = 11.84 RR24 pKa = 11.84 PTNASSEE31 pKa = 4.49 TATSVSSNTSFPATVAHH48 pKa = 6.81 LFYY51 pKa = 11.21 VVGDD55 pKa = 3.78 SFDD58 pKa = 4.15 FFVGDD63 pKa = 3.86 TFVPCYY69 pKa = 10.73 AEE71 pKa = 4.04 FVRR74 pKa = 11.84 VFEE77 pKa = 4.76 PGSRR81 pKa = 11.84 CYY83 pKa = 11.02 AWFVSPAVEE92 pKa = 3.89 FRR94 pKa = 11.84 FRR96 pKa = 11.84 VDD98 pKa = 3.26 TLPLGGRR105 pKa = 11.84 RR106 pKa = 11.84 AVRR109 pKa = 11.84 ALRR112 pKa = 11.84 RR113 pKa = 11.84 RR114 pKa = 11.84 LSSCFSRR121 pKa = 11.84 RR122 pKa = 11.84 AALLASPAQLARR134 pKa = 11.84 TRR136 pKa = 11.84 AVRR139 pKa = 11.84 FVRR142 pKa = 11.84 ASEE145 pKa = 4.39 GGPEE149 pKa = 4.03 PKK151 pKa = 9.98 SAEE154 pKa = 3.98 RR155 pKa = 11.84 APVCDD160 pKa = 3.42 NRR162 pKa = 11.84 FFSPRR167 pKa = 11.84 AVEE170 pKa = 4.01 SLSGFEE176 pKa = 4.06 HH177 pKa = 6.54 AVVGTRR183 pKa = 11.84 LEE185 pKa = 4.36 APASVWLRR193 pKa = 11.84 EE194 pKa = 3.93 EE195 pKa = 4.74 GVRR198 pKa = 11.84 AWGSVSDD205 pKa = 3.93 LSARR209 pKa = 11.84 GEE211 pKa = 3.83 RR212 pKa = 11.84 RR213 pKa = 11.84 AALSSRR219 pKa = 11.84 FLSAVTPWSPEE230 pKa = 3.66 LPLQKK235 pKa = 9.71 PRR237 pKa = 11.84 AVVRR241 pKa = 11.84 PPTSVAPLVLQAAVAAPVLRR261 pKa = 11.84 TEE263 pKa = 4.06 VAFDD267 pKa = 3.48 SGALASVWGPRR278 pKa = 11.84 RR279 pKa = 11.84 EE280 pKa = 3.97 EE281 pKa = 4.07 SALGPGASGSGAGGFAALEE300 pKa = 4.13 ASLAPSVRR308 pKa = 11.84 APSGGGFAALEE319 pKa = 4.16 ARR321 pKa = 11.84 TAALGSSGLLGGGCGAVGSRR341 pKa = 11.84 SFSSAQGTRR350 pKa = 11.84 VGSLSGEE357 pKa = 4.18 CATRR361 pKa = 11.84 EE362 pKa = 4.07 VSDD365 pKa = 3.3 VTVTVEE371 pKa = 4.32 PPADD375 pKa = 3.67 DD376 pKa = 4.32 SFTANRR382 pKa = 11.84 ALPSFRR388 pKa = 11.84 EE389 pKa = 4.24 VVRR392 pKa = 11.84 SSLGASDD399 pKa = 4.86 DD400 pKa = 4.03 GSGGLAAVGVPSSLRR415 pKa = 11.84 RR416 pKa = 11.84 RR417 pKa = 11.84 RR418 pKa = 11.84 GFACHH423 pKa = 6.54 KK424 pKa = 10.15 CGAQFSDD431 pKa = 3.54 ALVLSTHH438 pKa = 6.42 TSGCPNHH445 pKa = 6.93 RR446 pKa = 11.84 CICGKK451 pKa = 9.2 PCRR454 pKa = 11.84 THH456 pKa = 6.68 AQRR459 pKa = 11.84 QEE461 pKa = 3.91 CVGRR465 pKa = 11.84 HH466 pKa = 4.68 FAGEE470 pKa = 4.07 PFPCPVCFSGSFRR483 pKa = 11.84 TFAEE487 pKa = 4.15 LCAHH491 pKa = 5.8 VRR493 pKa = 11.84 EE494 pKa = 4.67 APCRR498 pKa = 11.84 RR499 pKa = 11.84 LEE501 pKa = 4.27 LGRR504 pKa = 11.84 ASS506 pKa = 3.39
Molecular weight: 53.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 8.902
IPC_protein 9.224
Toseland 9.765
ProMoST 9.648
Dawson 9.999
Bjellqvist 9.94
Wikipedia 10.189
Rodwell 9.999
Grimsley 10.014
Solomon 10.116
Lehninger 10.087
Nozaki 10.218
DTASelect 9.794
Thurlkill 9.94
EMBOSS 10.233
Sillero 10.087
Patrickios 9.385
IPC_peptide 10.101
IPC2_peptide 9.706
IPC2.peptide.svr19 7.785
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4224
89
1132
603.4
65.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.559 ± 0.57
1.823 ± 0.262
4.948 ± 0.338
5.729 ± 0.473
3.291 ± 0.554
8.333 ± 0.644
2.675 ± 0.361
2.841 ± 0.62
2.936 ± 0.544
9.446 ± 0.498
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.32 ± 0.505
2.509 ± 0.33
5.208 ± 0.501
3.741 ± 0.619
7.434 ± 0.816
7.647 ± 0.834
5.729 ± 0.447
8.57 ± 0.572
1.231 ± 0.093
3.03 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here