Hafnia phage vB_HpaA_yong1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MGX8|A0A482MGX8_9CAUD Uncharacterized protein OS=Hafnia phage vB_HpaA_yong1 OX=2562199 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVKK17 pKa = 10.72AFNEE21 pKa = 4.58RR22 pKa = 11.84IQYY25 pKa = 10.62DD26 pKa = 4.32HH27 pKa = 7.02LTQDD31 pKa = 4.22DD32 pKa = 4.81DD33 pKa = 4.96YY34 pKa = 11.8SDD36 pKa = 4.28ALHH39 pKa = 6.44EE40 pKa = 4.6VVDD43 pKa = 4.57DD44 pKa = 4.1MVPHH48 pKa = 6.78YY49 pKa = 9.72YY50 pKa = 10.36HH51 pKa = 7.58EE52 pKa = 4.82IFTVMAADD60 pKa = 5.4GIDD63 pKa = 3.84HH64 pKa = 6.56EE65 pKa = 5.23FEE67 pKa = 5.3DD68 pKa = 4.36SGLMPEE74 pKa = 4.55TKK76 pKa = 10.2DD77 pKa = 3.18VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 7.14EE88 pKa = 4.21TLYY91 pKa = 11.06NDD93 pKa = 3.45VSNSSDD99 pKa = 3.74VVWFEE104 pKa = 4.38AEE106 pKa = 3.95EE107 pKa = 4.14SDD109 pKa = 5.33EE110 pKa = 4.06EE111 pKa = 4.2DD112 pKa = 3.93EE113 pKa = 4.55YY114 pKa = 11.13WVVDD118 pKa = 3.3ARR120 pKa = 11.84TGVIIEE126 pKa = 4.18QAVPLNDD133 pKa = 5.73AITCAKK139 pKa = 10.24DD140 pKa = 3.55LYY142 pKa = 11.3AVGHH146 pKa = 5.51HH147 pKa = 5.99MKK149 pKa = 10.43VEE151 pKa = 4.77DD152 pKa = 3.7INDD155 pKa = 3.41NVVFDD160 pKa = 4.29PAAAEE165 pKa = 4.13EE166 pKa = 4.35DD167 pKa = 4.35CEE169 pKa = 4.21

Molecular weight: 19.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MH56|A0A482MH56_9CAUD Uncharacterized protein OS=Hafnia phage vB_HpaA_yong1 OX=2562199 PE=4 SV=1
MM1 pKa = 6.5VTYY4 pKa = 9.07GLCKK8 pKa = 10.1HH9 pKa = 6.32HH10 pKa = 6.31VTNARR15 pKa = 11.84IMVKK19 pKa = 9.29TGKK22 pKa = 9.65FKK24 pKa = 10.87HH25 pKa = 6.16DD26 pKa = 2.96AAMRR30 pKa = 11.84LLKK33 pKa = 10.4VVYY36 pKa = 9.77GRR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.48RR41 pKa = 11.84MHH43 pKa = 7.19DD44 pKa = 3.6SLHH47 pKa = 6.84AEE49 pKa = 4.03

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12055

45

1295

241.1

26.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.669 ± 0.416

1.054 ± 0.2

6.429 ± 0.287

6.869 ± 0.437

3.716 ± 0.182

7.905 ± 0.353

1.759 ± 0.207

4.828 ± 0.213

6.595 ± 0.434

8.096 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.186

4.571 ± 0.284

3.633 ± 0.173

3.849 ± 0.306

5.5 ± 0.229

6.064 ± 0.375

5.699 ± 0.317

7.184 ± 0.282

1.402 ± 0.16

3.393 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski