Achromobacter phage JWAlpha

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rothmandenesvirinae; Jwalphavirus; Achromobacter virus JWAlpha

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9VF66|V9VF66_9CAUD Uncharacterized protein OS=Achromobacter phage JWAlpha OX=1416009 GN=JJJB_0032 PE=4 SV=1
MM1 pKa = 7.43WHH3 pKa = 6.61DD4 pKa = 3.29QYY6 pKa = 11.49PRR8 pKa = 11.84SEE10 pKa = 3.98HH11 pKa = 4.73QARR14 pKa = 11.84VLLGDD19 pKa = 4.15PVALAYY25 pKa = 9.95EE26 pKa = 4.68WISRR30 pKa = 11.84YY31 pKa = 10.14AEE33 pKa = 3.95NLTVQVQKK41 pKa = 11.21YY42 pKa = 10.03NEE44 pKa = 4.17DD45 pKa = 3.54DD46 pKa = 3.74EE47 pKa = 4.96YY48 pKa = 11.68GNNEE52 pKa = 5.67DD53 pKa = 3.73ITADD57 pKa = 3.97DD58 pKa = 5.58LISTADD64 pKa = 3.28SHH66 pKa = 7.45QGDD69 pKa = 3.01RR70 pKa = 11.84WGDD73 pKa = 3.51YY74 pKa = 9.93ISRR77 pKa = 11.84GGTFEE82 pKa = 4.53GEE84 pKa = 4.49SVDD87 pKa = 3.44PTFWKK92 pKa = 10.29HH93 pKa = 3.83YY94 pKa = 8.77ATIKK98 pKa = 10.11GLEE101 pKa = 4.17VEE103 pKa = 5.42DD104 pKa = 4.32VVQSSFFSCYY114 pKa = 9.61CC115 pKa = 3.74

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9VCT7|V9VCT7_9CAUD Uncharacterized protein OS=Achromobacter phage JWAlpha OX=1416009 GN=JJJB_0014 PE=4 SV=1
MM1 pKa = 7.42SLYY4 pKa = 11.08AFLNFLMVIAVGGAIGVLCWWFKK27 pKa = 11.33SRR29 pKa = 11.84DD30 pKa = 3.42FTKK33 pKa = 10.66RR34 pKa = 11.84ITAGARR40 pKa = 11.84GLYY43 pKa = 10.39AIVLFRR49 pKa = 11.84LFRR52 pKa = 11.84CVIVGMALGLLVATFISWW70 pKa = 2.99

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

22967

29

3425

252.4

27.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.522 ± 0.621

0.919 ± 0.158

6.043 ± 0.126

5.939 ± 0.251

3.448 ± 0.185

7.241 ± 0.302

2.181 ± 0.203

5.107 ± 0.311

5.769 ± 0.262

8.255 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.305 ± 0.126

4.711 ± 0.241

4.506 ± 0.191

4.794 ± 0.263

5.081 ± 0.191

5.908 ± 0.187

6.07 ± 0.207

6.818 ± 0.216

1.328 ± 0.129

3.052 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski