Pseudomonas phage DL64

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Migulavirinae; Litunavirus; unclassified Litunavirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6WDN6|A0A0F6WDN6_9CAUD ATP-dependent Clp protease ATP-binding subunit ClpX OS=Pseudomonas phage DL64 OX=1640973 PE=4 SV=1
MM1 pKa = 7.89TSTCWVNDD9 pKa = 3.36HH10 pKa = 7.09EE11 pKa = 4.74ITVSQGVVAVRR22 pKa = 11.84KK23 pKa = 10.39DD24 pKa = 3.18GVLVHH29 pKa = 6.46EE30 pKa = 5.47CGGLWAACDD39 pKa = 3.69FARR42 pKa = 11.84GINSAEE48 pKa = 4.06NPGPEE53 pKa = 3.76VDD55 pKa = 3.93EE56 pKa = 4.42VQEE59 pKa = 4.37TVHH62 pKa = 6.58VDD64 pKa = 3.12SDD66 pKa = 3.67EE67 pKa = 4.61CGQAEE72 pKa = 4.49CSS74 pKa = 3.53

Molecular weight: 7.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6WDQ1|A0A0F6WDQ1_9CAUD Uncharacterized protein OS=Pseudomonas phage DL64 OX=1640973 PE=4 SV=1
MM1 pKa = 6.86NTNHH5 pKa = 6.69NLTLVGRR12 pKa = 11.84LYY14 pKa = 10.56KK15 pKa = 10.36IKK17 pKa = 10.45IIPTKK22 pKa = 10.72GKK24 pKa = 8.41LTGFRR29 pKa = 11.84VQVTLINKK37 pKa = 7.72GFPDD41 pKa = 3.72TVLTHH46 pKa = 6.57VVMCGDD52 pKa = 4.22EE53 pKa = 4.32YY54 pKa = 10.67TAKK57 pKa = 9.84TLTSAIRR64 pKa = 11.84NRR66 pKa = 11.84RR67 pKa = 11.84QLNLKK72 pKa = 7.73HH73 pKa = 5.31WHH75 pKa = 5.54WAGVNKK81 pKa = 10.02DD82 pKa = 3.56VPGACTNRR90 pKa = 11.84LKK92 pKa = 10.12TKK94 pKa = 8.44PTTLEE99 pKa = 3.62II100 pKa = 4.54

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

22247

46

3398

247.2

27.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.143 ± 0.533

0.921 ± 0.148

6.037 ± 0.184

6.954 ± 0.315

3.794 ± 0.188

6.738 ± 0.25

1.892 ± 0.189

4.985 ± 0.257

5.915 ± 0.26

8.9 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.12 ± 0.149

4.603 ± 0.179

3.974 ± 0.216

4.482 ± 0.283

5.385 ± 0.237

5.677 ± 0.194

5.929 ± 0.269

7.048 ± 0.236

1.295 ± 0.135

3.209 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski