Amino acid dipepetide frequency for Serratia phage CBH8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.151AlaAla: 5.151 ± 0.391
0.468AlaCys: 0.468 ± 0.099
3.877AlaAsp: 3.877 ± 0.262
5.076AlaGlu: 5.076 ± 0.387
2.772AlaPhe: 2.772 ± 0.227
4.476AlaGly: 4.476 ± 0.356
0.993AlaHis: 0.993 ± 0.15
4.982AlaIle: 4.982 ± 0.254
5.151AlaLys: 5.151 ± 0.323
5.994AlaLeu: 5.994 ± 0.355
2.06AlaMet: 2.06 ± 0.203
3.128AlaAsn: 3.128 ± 0.243
2.51AlaPro: 2.51 ± 0.214
2.547AlaGln: 2.547 ± 0.215
3.409AlaArg: 3.409 ± 0.25
4.439AlaSer: 4.439 ± 0.256
3.765AlaThr: 3.765 ± 0.372
4.439AlaVal: 4.439 ± 0.283
0.955AlaTrp: 0.955 ± 0.122
2.566AlaTyr: 2.566 ± 0.203
0.0AlaXaa: 0.0 ± 0.0
Cys
0.712CysAla: 0.712 ± 0.148
0.225CysCys: 0.225 ± 0.063
0.936CysAsp: 0.936 ± 0.143
0.749CysGlu: 0.749 ± 0.143
0.375CysPhe: 0.375 ± 0.078
0.637CysGly: 0.637 ± 0.106
0.356CysHis: 0.356 ± 0.085
0.618CysIle: 0.618 ± 0.122
0.524CysLys: 0.524 ± 0.101
0.562CysLeu: 0.562 ± 0.096
0.356CysMet: 0.356 ± 0.083
0.562CysAsn: 0.562 ± 0.091
0.506CysPro: 0.506 ± 0.1
0.337CysGln: 0.337 ± 0.08
0.562CysArg: 0.562 ± 0.103
1.068CysSer: 1.068 ± 0.133
0.637CysThr: 0.637 ± 0.116
0.73CysVal: 0.73 ± 0.115
0.112CysTrp: 0.112 ± 0.047
0.337CysTyr: 0.337 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
3.915AspAla: 3.915 ± 0.339
0.693AspCys: 0.693 ± 0.103
3.783AspAsp: 3.783 ± 0.297
4.982AspGlu: 4.982 ± 0.307
3.559AspPhe: 3.559 ± 0.266
4.757AspGly: 4.757 ± 0.295
1.011AspHis: 1.011 ± 0.126
4.439AspIle: 4.439 ± 0.293
4.645AspLys: 4.645 ± 0.337
4.963AspLeu: 4.963 ± 0.293
1.573AspMet: 1.573 ± 0.165
3.184AspAsn: 3.184 ± 0.235
2.416AspPro: 2.416 ± 0.219
1.592AspGln: 1.592 ± 0.176
2.079AspArg: 2.079 ± 0.219
3.933AspSer: 3.933 ± 0.24
3.465AspThr: 3.465 ± 0.276
4.383AspVal: 4.383 ± 0.269
1.33AspTrp: 1.33 ± 0.174
3.034AspTyr: 3.034 ± 0.248
0.0AspXaa: 0.0 ± 0.0
Glu
5.713GluAla: 5.713 ± 0.311
0.768GluCys: 0.768 ± 0.106
4.439GluAsp: 4.439 ± 0.306
5.375GluGlu: 5.375 ± 0.374
3.315GluPhe: 3.315 ± 0.268
4.064GluGly: 4.064 ± 0.273
1.367GluHis: 1.367 ± 0.156
5.787GluIle: 5.787 ± 0.327
4.982GluLys: 4.982 ± 0.319
7.005GluLeu: 7.005 ± 0.387
2.266GluMet: 2.266 ± 0.214
3.39GluAsn: 3.39 ± 0.245
1.892GluPro: 1.892 ± 0.176
2.454GluGln: 2.454 ± 0.204
3.109GluArg: 3.109 ± 0.227
3.84GluSer: 3.84 ± 0.291
3.896GluThr: 3.896 ± 0.28
5.113GluVal: 5.113 ± 0.283
0.993GluTrp: 0.993 ± 0.146
3.109GluTyr: 3.109 ± 0.219
0.0GluXaa: 0.0 ± 0.0
Phe
2.791PheAla: 2.791 ± 0.242
0.393PheCys: 0.393 ± 0.088
3.222PheAsp: 3.222 ± 0.227
3.634PheGlu: 3.634 ± 0.312
1.536PhePhe: 1.536 ± 0.194
3.147PheGly: 3.147 ± 0.254
0.73PheHis: 0.73 ± 0.135
3.296PheIle: 3.296 ± 0.243
4.027PheLys: 4.027 ± 0.267
2.266PheLeu: 2.266 ± 0.207
1.311PheMet: 1.311 ± 0.173
2.585PheAsn: 2.585 ± 0.221
1.049PhePro: 1.049 ± 0.131
1.311PheGln: 1.311 ± 0.166
1.948PheArg: 1.948 ± 0.202
2.585PheSer: 2.585 ± 0.175
2.416PheThr: 2.416 ± 0.237
3.015PheVal: 3.015 ± 0.233
0.749PheTrp: 0.749 ± 0.13
1.573PheTyr: 1.573 ± 0.138
0.0PheXaa: 0.0 ± 0.0
Gly
3.577GlyAla: 3.577 ± 0.293
0.73GlyCys: 0.73 ± 0.146
4.046GlyAsp: 4.046 ± 0.333
4.214GlyGlu: 4.214 ± 0.282
3.278GlyPhe: 3.278 ± 0.283
3.615GlyGly: 3.615 ± 0.329
1.461GlyHis: 1.461 ± 0.144
4.327GlyIle: 4.327 ± 0.307
4.645GlyLys: 4.645 ± 0.335
4.795GlyLeu: 4.795 ± 0.319
2.042GlyMet: 2.042 ± 0.21
3.128GlyAsn: 3.128 ± 0.412
1.555GlyPro: 1.555 ± 0.171
1.967GlyGln: 1.967 ± 0.21
2.959GlyArg: 2.959 ± 0.26
4.289GlySer: 4.289 ± 0.29
4.121GlyThr: 4.121 ± 0.311
4.308GlyVal: 4.308 ± 0.296
1.18GlyTrp: 1.18 ± 0.155
2.884GlyTyr: 2.884 ± 0.227
0.0GlyXaa: 0.0 ± 0.0
His
1.068HisAla: 1.068 ± 0.151
0.375HisCys: 0.375 ± 0.101
1.161HisAsp: 1.161 ± 0.14
1.03HisGlu: 1.03 ± 0.143
0.768HisPhe: 0.768 ± 0.12
1.068HisGly: 1.068 ± 0.131
0.45HisHis: 0.45 ± 0.095
1.517HisIle: 1.517 ± 0.169
1.723HisLys: 1.723 ± 0.177
1.667HisLeu: 1.667 ± 0.186
0.524HisMet: 0.524 ± 0.106
0.862HisAsn: 0.862 ± 0.128
0.955HisPro: 0.955 ± 0.135
0.581HisGln: 0.581 ± 0.104
0.787HisArg: 0.787 ± 0.123
1.143HisSer: 1.143 ± 0.142
0.88HisThr: 0.88 ± 0.129
1.143HisVal: 1.143 ± 0.147
0.262HisTrp: 0.262 ± 0.072
0.805HisTyr: 0.805 ± 0.127
0.0HisXaa: 0.0 ± 0.0
Ile
4.308IleAla: 4.308 ± 0.292
0.824IleCys: 0.824 ± 0.126
5.469IleAsp: 5.469 ± 0.312
5.563IleGlu: 5.563 ± 0.308
2.229IlePhe: 2.229 ± 0.183
3.634IleGly: 3.634 ± 0.285
1.255IleHis: 1.255 ± 0.14
4.42IleIle: 4.42 ± 0.279
6.106IleLys: 6.106 ± 0.328
4.701IleLeu: 4.701 ± 0.363
1.854IleMet: 1.854 ± 0.18
4.102IleAsn: 4.102 ± 0.307
2.978IlePro: 2.978 ± 0.268
2.248IleGln: 2.248 ± 0.205
3.54IleArg: 3.54 ± 0.285
4.645IleSer: 4.645 ± 0.282
4.533IleThr: 4.533 ± 0.262
3.858IleVal: 3.858 ± 0.281
0.487IleTrp: 0.487 ± 0.1
2.603IleTyr: 2.603 ± 0.216
0.0IleXaa: 0.0 ± 0.0
Lys
6.612LysAla: 6.612 ± 0.397
0.749LysCys: 0.749 ± 0.104
4.72LysAsp: 4.72 ± 0.332
5.713LysGlu: 5.713 ± 0.347
3.708LysPhe: 3.708 ± 0.248
4.439LysGly: 4.439 ± 0.247
1.536LysHis: 1.536 ± 0.168
5.151LysIle: 5.151 ± 0.343
4.888LysLys: 4.888 ± 0.373
5.9LysLeu: 5.9 ± 0.42
2.472LysMet: 2.472 ± 0.193
3.989LysAsn: 3.989 ± 0.292
2.435LysPro: 2.435 ± 0.203
2.472LysGln: 2.472 ± 0.213
3.428LysArg: 3.428 ± 0.247
4.308LysSer: 4.308 ± 0.328
4.177LysThr: 4.177 ± 0.269
4.926LysVal: 4.926 ± 0.312
1.124LysTrp: 1.124 ± 0.143
3.203LysTyr: 3.203 ± 0.256
0.0LysXaa: 0.0 ± 0.0
Leu
5.619LeuAla: 5.619 ± 0.457
0.637LeuCys: 0.637 ± 0.111
4.795LeuAsp: 4.795 ± 0.317
5.469LeuGlu: 5.469 ± 0.346
3.09LeuPhe: 3.09 ± 0.244
4.439LeuGly: 4.439 ± 0.287
1.255LeuHis: 1.255 ± 0.143
4.963LeuIle: 4.963 ± 0.273
6.312LeuLys: 6.312 ± 0.317
4.701LeuLeu: 4.701 ± 0.305
2.285LeuMet: 2.285 ± 0.187
4.439LeuAsn: 4.439 ± 0.248
3.053LeuPro: 3.053 ± 0.216
2.978LeuGln: 2.978 ± 0.224
3.765LeuArg: 3.765 ± 0.261
4.982LeuSer: 4.982 ± 0.292
4.214LeuThr: 4.214 ± 0.276
4.963LeuVal: 4.963 ± 0.275
0.955LeuTrp: 0.955 ± 0.134
2.566LeuTyr: 2.566 ± 0.25
0.0LeuXaa: 0.0 ± 0.0
Met
2.36MetAla: 2.36 ± 0.224
0.356MetCys: 0.356 ± 0.078
1.836MetAsp: 1.836 ± 0.156
1.555MetGlu: 1.555 ± 0.17
1.236MetPhe: 1.236 ± 0.176
1.367MetGly: 1.367 ± 0.153
0.45MetHis: 0.45 ± 0.088
1.892MetIle: 1.892 ± 0.167
2.697MetLys: 2.697 ± 0.249
2.285MetLeu: 2.285 ± 0.176
0.88MetMet: 0.88 ± 0.133
2.098MetAsn: 2.098 ± 0.194
0.805MetPro: 0.805 ± 0.115
1.199MetGln: 1.199 ± 0.138
1.068MetArg: 1.068 ± 0.136
2.585MetSer: 2.585 ± 0.228
1.704MetThr: 1.704 ± 0.192
1.667MetVal: 1.667 ± 0.161
0.318MetTrp: 0.318 ± 0.069
1.049MetTyr: 1.049 ± 0.13
0.0MetXaa: 0.0 ± 0.0
Asn
3.409AsnAla: 3.409 ± 0.281
0.562AsnCys: 0.562 ± 0.107
3.203AsnAsp: 3.203 ± 0.25
3.877AsnGlu: 3.877 ± 0.27
2.454AsnPhe: 2.454 ± 0.219
3.896AsnGly: 3.896 ± 0.418
0.974AsnHis: 0.974 ± 0.127
3.502AsnIle: 3.502 ± 0.27
3.634AsnLys: 3.634 ± 0.212
4.214AsnLeu: 4.214 ± 0.266
1.573AsnMet: 1.573 ± 0.175
2.828AsnAsn: 2.828 ± 0.252
2.191AsnPro: 2.191 ± 0.214
1.892AsnGln: 1.892 ± 0.219
2.529AsnArg: 2.529 ± 0.209
2.772AsnSer: 2.772 ± 0.26
2.735AsnThr: 2.735 ± 0.227
3.259AsnVal: 3.259 ± 0.314
0.618AsnTrp: 0.618 ± 0.093
2.042AsnTyr: 2.042 ± 0.198
0.0AsnXaa: 0.0 ± 0.0
Pro
2.116ProAla: 2.116 ± 0.194
0.45ProCys: 0.45 ± 0.102
2.491ProAsp: 2.491 ± 0.217
2.997ProGlu: 2.997 ± 0.203
1.48ProPhe: 1.48 ± 0.172
2.66ProGly: 2.66 ± 0.244
0.581ProHis: 0.581 ± 0.091
2.21ProIle: 2.21 ± 0.199
2.491ProLys: 2.491 ± 0.247
2.435ProLeu: 2.435 ± 0.192
0.787ProMet: 0.787 ± 0.112
2.004ProAsn: 2.004 ± 0.187
0.843ProPro: 0.843 ± 0.132
0.936ProGln: 0.936 ± 0.122
1.517ProArg: 1.517 ± 0.173
2.379ProSer: 2.379 ± 0.217
2.042ProThr: 2.042 ± 0.229
2.622ProVal: 2.622 ± 0.204
0.581ProTrp: 0.581 ± 0.113
1.236ProTyr: 1.236 ± 0.173
0.0ProXaa: 0.0 ± 0.0
Gln
2.454GlnAla: 2.454 ± 0.189
0.281GlnCys: 0.281 ± 0.083
1.873GlnAsp: 1.873 ± 0.195
2.36GlnGlu: 2.36 ± 0.222
1.555GlnPhe: 1.555 ± 0.171
1.91GlnGly: 1.91 ± 0.192
0.805GlnHis: 0.805 ± 0.142
2.379GlnIle: 2.379 ± 0.201
2.416GlnLys: 2.416 ± 0.257
2.847GlnLeu: 2.847 ± 0.218
0.993GlnMet: 0.993 ± 0.121
1.349GlnAsn: 1.349 ± 0.156
1.049GlnPro: 1.049 ± 0.146
1.068GlnGln: 1.068 ± 0.124
1.779GlnArg: 1.779 ± 0.198
2.098GlnSer: 2.098 ± 0.211
2.098GlnThr: 2.098 ± 0.207
2.248GlnVal: 2.248 ± 0.196
0.73GlnTrp: 0.73 ± 0.117
1.48GlnTyr: 1.48 ± 0.21
0.0GlnXaa: 0.0 ± 0.0
Arg
2.978ArgAla: 2.978 ± 0.259
0.468ArgCys: 0.468 ± 0.097
2.828ArgAsp: 2.828 ± 0.239
3.502ArgGlu: 3.502 ± 0.253
2.042ArgPhe: 2.042 ± 0.201
3.034ArgGly: 3.034 ± 0.231
0.918ArgHis: 0.918 ± 0.148
3.54ArgIle: 3.54 ± 0.246
3.259ArgLys: 3.259 ± 0.263
3.577ArgLeu: 3.577 ± 0.242
1.255ArgMet: 1.255 ± 0.147
2.135ArgAsn: 2.135 ± 0.182
1.33ArgPro: 1.33 ± 0.165
1.592ArgGln: 1.592 ± 0.173
2.248ArgArg: 2.248 ± 0.249
2.697ArgSer: 2.697 ± 0.228
2.791ArgThr: 2.791 ± 0.233
2.997ArgVal: 2.997 ± 0.274
0.787ArgTrp: 0.787 ± 0.139
1.798ArgTyr: 1.798 ± 0.191
0.0ArgXaa: 0.0 ± 0.0
Ser
4.195SerAla: 4.195 ± 0.31
0.712SerCys: 0.712 ± 0.136
3.727SerAsp: 3.727 ± 0.288
4.289SerGlu: 4.289 ± 0.319
2.622SerPhe: 2.622 ± 0.254
4.982SerGly: 4.982 ± 0.295
1.124SerHis: 1.124 ± 0.165
4.27SerIle: 4.27 ± 0.232
4.926SerLys: 4.926 ± 0.27
4.776SerLeu: 4.776 ± 0.309
2.079SerMet: 2.079 ± 0.177
2.903SerAsn: 2.903 ± 0.239
2.229SerPro: 2.229 ± 0.236
1.985SerGln: 1.985 ± 0.2
3.315SerArg: 3.315 ± 0.254
4.195SerSer: 4.195 ± 0.268
3.915SerThr: 3.915 ± 0.352
4.064SerVal: 4.064 ± 0.327
0.899SerTrp: 0.899 ± 0.141
2.341SerTyr: 2.341 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
3.577ThrAla: 3.577 ± 0.262
0.637ThrCys: 0.637 ± 0.098
3.296ThrAsp: 3.296 ± 0.253
4.158ThrGlu: 4.158 ± 0.273
2.435ThrPhe: 2.435 ± 0.238
4.046ThrGly: 4.046 ± 0.32
1.143ThrHis: 1.143 ± 0.125
3.802ThrIle: 3.802 ± 0.273
4.083ThrLys: 4.083 ± 0.3
4.139ThrLeu: 4.139 ± 0.278
1.255ThrMet: 1.255 ± 0.139
2.566ThrAsn: 2.566 ± 0.227
2.753ThrPro: 2.753 ± 0.264
2.135ThrGln: 2.135 ± 0.232
2.435ThrArg: 2.435 ± 0.215
4.121ThrSer: 4.121 ± 0.331
3.109ThrThr: 3.109 ± 0.249
4.42ThrVal: 4.42 ± 0.304
0.899ThrTrp: 0.899 ± 0.147
2.116ThrTyr: 2.116 ± 0.195
0.0ThrXaa: 0.0 ± 0.0
Val
4.589ValAla: 4.589 ± 0.267
0.88ValCys: 0.88 ± 0.146
4.233ValAsp: 4.233 ± 0.222
4.888ValGlu: 4.888 ± 0.317
2.735ValPhe: 2.735 ± 0.234
3.746ValGly: 3.746 ± 0.359
1.18ValHis: 1.18 ± 0.15
4.701ValIle: 4.701 ± 0.319
5.488ValLys: 5.488 ± 0.296
4.608ValLeu: 4.608 ± 0.291
2.154ValMet: 2.154 ± 0.18
3.671ValAsn: 3.671 ± 0.22
2.173ValPro: 2.173 ± 0.198
2.285ValGln: 2.285 ± 0.196
3.015ValArg: 3.015 ± 0.239
4.195ValSer: 4.195 ± 0.331
3.671ValThr: 3.671 ± 0.323
4.533ValVal: 4.533 ± 0.297
0.974ValTrp: 0.974 ± 0.13
2.66ValTyr: 2.66 ± 0.245
0.0ValXaa: 0.0 ± 0.0
Trp
1.011TrpAla: 1.011 ± 0.151
0.225TrpCys: 0.225 ± 0.064
1.105TrpAsp: 1.105 ± 0.125
0.936TrpGlu: 0.936 ± 0.131
0.73TrpPhe: 0.73 ± 0.125
0.562TrpGly: 0.562 ± 0.104
0.3TrpHis: 0.3 ± 0.09
0.693TrpIle: 0.693 ± 0.116
1.236TrpLys: 1.236 ± 0.163
1.255TrpLeu: 1.255 ± 0.145
0.712TrpMet: 0.712 ± 0.107
0.768TrpAsn: 0.768 ± 0.125
0.487TrpPro: 0.487 ± 0.098
0.506TrpGln: 0.506 ± 0.102
0.637TrpArg: 0.637 ± 0.114
0.862TrpSer: 0.862 ± 0.135
0.805TrpThr: 0.805 ± 0.131
1.049TrpVal: 1.049 ± 0.151
0.225TrpTrp: 0.225 ± 0.061
0.73TrpTyr: 0.73 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.603TyrAla: 2.603 ± 0.265
0.468TyrCys: 0.468 ± 0.084
2.697TyrAsp: 2.697 ± 0.255
2.51TyrGlu: 2.51 ± 0.244
1.667TyrPhe: 1.667 ± 0.172
2.641TyrGly: 2.641 ± 0.253
0.88TyrHis: 0.88 ± 0.113
2.866TyrIle: 2.866 ± 0.209
2.716TyrLys: 2.716 ± 0.22
2.791TyrLeu: 2.791 ± 0.239
0.955TyrMet: 0.955 ± 0.137
2.491TyrAsn: 2.491 ± 0.231
1.648TyrPro: 1.648 ± 0.194
1.686TyrGln: 1.686 ± 0.195
1.648TyrArg: 1.648 ± 0.2
2.416TyrSer: 2.416 ± 0.24
2.154TyrThr: 2.154 ± 0.206
2.641TyrVal: 2.641 ± 0.23
0.656TyrTrp: 0.656 ± 0.114
1.48TyrTyr: 1.48 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 275 proteins (53392 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski