Odonata-associated circular virus-13
Average proteome isoelectric point is 8.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UGT7|A0A0B4UGT7_9VIRU Putative capsid protein OS=Odonata-associated circular virus-13 OX=1592113 PE=4 SV=1
MM1 pKa = 7.48 GSNVLRR7 pKa = 11.84 AKK9 pKa = 10.37 NGSSGPRR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 10.16 YY19 pKa = 10.64 KK20 pKa = 10.48 LYY22 pKa = 10.34 CFTSFAVDD30 pKa = 3.25 QEE32 pKa = 4.22 PSYY35 pKa = 11.61 DD36 pKa = 3.73 PTAMQYY42 pKa = 10.92 LIYY45 pKa = 10.62 GRR47 pKa = 11.84 EE48 pKa = 3.89 TCPDD52 pKa = 3.1 SGRR55 pKa = 11.84 THH57 pKa = 6.61 LQGFVAFKK65 pKa = 9.95 NRR67 pKa = 11.84 QYY69 pKa = 10.15 FTACKK74 pKa = 9.76 KK75 pKa = 10.65 YY76 pKa = 10.13 FGTAHH81 pKa = 6.45 VEE83 pKa = 3.95 ACKK86 pKa = 9.95 GTFAEE91 pKa = 4.12 NQEE94 pKa = 4.2 YY95 pKa = 10.2 CKK97 pKa = 10.6 KK98 pKa = 10.86 DD99 pKa = 3.0 GDD101 pKa = 3.97 YY102 pKa = 11.34 KK103 pKa = 11.18 EE104 pKa = 5.56 FGTAPEE110 pKa = 4.32 IKK112 pKa = 10.34 SGGDD116 pKa = 3.09 VFNDD120 pKa = 3.29 VLRR123 pKa = 11.84 KK124 pKa = 10.15 AEE126 pKa = 4.3 AGSIQEE132 pKa = 4.76 IKK134 pKa = 10.65 DD135 pKa = 4.13 LYY137 pKa = 8.17 PGLYY141 pKa = 9.27 IRR143 pKa = 11.84 YY144 pKa = 8.93 KK145 pKa = 9.67 KK146 pKa = 8.93 TLEE149 pKa = 4.07 SIKK152 pKa = 10.52 RR153 pKa = 11.84 FNAEE157 pKa = 3.91 QLEE160 pKa = 4.45 EE161 pKa = 4.28 SCGIWLTGPPRR172 pKa = 11.84 SGKK175 pKa = 10.15 DD176 pKa = 3.33 YY177 pKa = 11.02 AVSTFFSSIYY187 pKa = 10.94 SKK189 pKa = 10.34 MLNKK193 pKa = 9.62 WFDD196 pKa = 3.44 GYY198 pKa = 11.04 EE199 pKa = 4.13 GEE201 pKa = 4.5 EE202 pKa = 4.3 CVHH205 pKa = 6.9 LSDD208 pKa = 3.54 MDD210 pKa = 3.98 KK211 pKa = 10.36 NHH213 pKa = 6.3 VYY215 pKa = 9.72 MGSFLKK221 pKa = 10.15 IWCDD225 pKa = 3.14 RR226 pKa = 11.84 YY227 pKa = 10.41 PFRR230 pKa = 11.84 AEE232 pKa = 3.65 IKK234 pKa = 9.86 GGTMVIRR241 pKa = 11.84 PKK243 pKa = 10.88 YY244 pKa = 9.87 IVVTSNYY251 pKa = 9.48 KK252 pKa = 10.67 LEE254 pKa = 5.3 DD255 pKa = 3.44 IFDD258 pKa = 3.97 GSMLSALQARR268 pKa = 11.84 FMVMCYY274 pKa = 10.59 DD275 pKa = 3.58 PVDD278 pKa = 3.97 GVVVTPRR285 pKa = 11.84 PVFQPSDD292 pKa = 3.33 RR293 pKa = 11.84 FIQALQHH300 pKa = 6.68 AIPKK304 pKa = 10.41 GEE306 pKa = 5.01 DD307 pKa = 3.42 VPDD310 pKa = 3.81 MAPKK314 pKa = 10.43 AGPSNALQEE323 pKa = 4.78 AEE325 pKa = 4.52 FSSSDD330 pKa = 3.45 EE331 pKa = 4.35 FKK333 pKa = 10.75 EE334 pKa = 4.16 SQVPKK339 pKa = 10.2 RR340 pKa = 11.84 KK341 pKa = 9.83 KK342 pKa = 10.31 KK343 pKa = 10.39 LL344 pKa = 3.32
Molecular weight: 39.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.109
IPC2_protein 7.497
IPC_protein 7.351
Toseland 6.956
ProMoST 7.907
Dawson 8.068
Bjellqvist 8.404
Wikipedia 7.995
Rodwell 8.083
Grimsley 6.971
Solomon 8.141
Lehninger 8.156
Nozaki 8.565
DTASelect 8.156
Thurlkill 8.214
EMBOSS 8.273
Sillero 8.536
Patrickios 4.101
IPC_peptide 8.126
IPC2_peptide 7.337
IPC2.peptide.svr19 7.605
Protein with the highest isoelectric point:
>tr|A0A0B4UGT7|A0A0B4UGT7_9VIRU Putative capsid protein OS=Odonata-associated circular virus-13 OX=1592113 PE=4 SV=1
MM1 pKa = 8.19 RR2 pKa = 11.84 YY3 pKa = 9.5 RR4 pKa = 11.84 RR5 pKa = 11.84 GKK7 pKa = 8.38 TYY9 pKa = 9.4 RR10 pKa = 11.84 TWRR13 pKa = 11.84 RR14 pKa = 11.84 KK15 pKa = 9.32 RR16 pKa = 11.84 GRR18 pKa = 11.84 LTRR21 pKa = 11.84 YY22 pKa = 8.74 KK23 pKa = 10.38 KK24 pKa = 10.31 RR25 pKa = 11.84 NFRR28 pKa = 11.84 LRR30 pKa = 11.84 MNSRR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 FQNEE41 pKa = 3.2 RR42 pKa = 11.84 RR43 pKa = 11.84 SFKK46 pKa = 10.22 STATYY51 pKa = 10.67 QLFYY55 pKa = 10.66 TVDD58 pKa = 4.23 LPTSDD63 pKa = 3.7 TPTVSNARR71 pKa = 11.84 YY72 pKa = 8.55 FQDD75 pKa = 3.39 SVKK78 pKa = 9.72 MFPLGSQLFNRR89 pKa = 11.84 YY90 pKa = 8.78 LRR92 pKa = 11.84 TYY94 pKa = 10.87 AWVKK98 pKa = 10.27 LNSVTYY104 pKa = 7.45 YY105 pKa = 9.8 WRR107 pKa = 11.84 IGFVGYY113 pKa = 10.82 NEE115 pKa = 4.36 AEE117 pKa = 4.37 KK118 pKa = 11.12 VPDD121 pKa = 4.54 PNDD124 pKa = 3.69 PQKK127 pKa = 11.05 KK128 pKa = 7.97 KK129 pKa = 10.57 QLVVMEE135 pKa = 4.8 AVNSLMGKK143 pKa = 9.95 FPFYY147 pKa = 11.03 LNWSLDD153 pKa = 3.13 GDD155 pKa = 4.43 NPTKK159 pKa = 10.33 MAMDD163 pKa = 5.15 DD164 pKa = 3.8 LAACPSSRR172 pKa = 11.84 KK173 pKa = 10.07 VYY175 pKa = 10.68 INGKK179 pKa = 9.12 KK180 pKa = 8.46 ATKK183 pKa = 8.56 FTYY186 pKa = 10.18 SIPKK190 pKa = 9.07 AYY192 pKa = 10.33 RR193 pKa = 11.84 MYY195 pKa = 10.76 LSSSVFRR202 pKa = 11.84 SMSVSSGIKK211 pKa = 10.46 NNITTLFNNSSLRR224 pKa = 11.84 CPDD227 pKa = 3.25 KK228 pKa = 11.34 FNGSMLDD235 pKa = 3.04 IFEE238 pKa = 4.85 KK239 pKa = 10.51 YY240 pKa = 10.63 AGVSLDD246 pKa = 3.58 NDD248 pKa = 3.92 MACEE252 pKa = 4.63 FILWCDD258 pKa = 3.25 VYY260 pKa = 10.57 MDD262 pKa = 4.56 CSFKK266 pKa = 11.1 GRR268 pKa = 11.84 IDD270 pKa = 3.47 NAA272 pKa = 3.81
Molecular weight: 32.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.575
IPC_protein 9.78
Toseland 10.072
ProMoST 9.823
Dawson 10.306
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.643
Grimsley 10.379
Solomon 10.321
Lehninger 10.277
Nozaki 10.087
DTASelect 9.999
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.218
Patrickios 10.014
IPC_peptide 10.321
IPC2_peptide 8.843
IPC2.peptide.svr19 8.543
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
616
272
344
308.0
35.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.844 ± 0.443
2.273 ± 0.276
6.006 ± 0.079
4.708 ± 1.589
6.169 ± 0.052
6.169 ± 1.116
0.812 ± 0.516
3.734 ± 0.504
8.604 ± 0.328
6.169 ± 0.519
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.571 ± 0.534
4.708 ± 1.447
4.87 ± 0.525
3.084 ± 0.558
6.981 ± 1.638
8.279 ± 0.579
4.87 ± 0.643
5.682 ± 0.106
1.299 ± 0.343
6.169 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here