Staphylococcus phage PMBT8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sextaecvirus; unclassified Sextaecvirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6E7K4|A0A4Y6E7K4_9CAUD HNHc domain-containing protein OS=Staphylococcus phage PMBT8 OX=2590894 PE=4 SV=1
MM1 pKa = 6.9NTNIDD6 pKa = 3.63VLNRR10 pKa = 11.84MLNKK14 pKa = 10.16KK15 pKa = 9.79GLSLSLEE22 pKa = 4.3SMDD25 pKa = 4.43GLTTFSPDD33 pKa = 2.91AQTYY37 pKa = 7.11EE38 pKa = 4.24VNGITIIDD46 pKa = 4.04DD47 pKa = 3.57VTCYY51 pKa = 9.96FVEE54 pKa = 4.73HH55 pKa = 5.72YY56 pKa = 10.22VAYY59 pKa = 10.5DD60 pKa = 3.55EE61 pKa = 4.68IEE63 pKa = 4.11AMNIITEE70 pKa = 4.15LLEE73 pKa = 4.3EE74 pKa = 4.35KK75 pKa = 10.71

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EID1|A0A4Y6EID1_9CAUD Uncharacterized protein OS=Staphylococcus phage PMBT8 OX=2590894 PE=4 SV=1
MM1 pKa = 6.92IQKK4 pKa = 9.6ARR6 pKa = 11.84KK7 pKa = 8.68KK8 pKa = 8.99PVEE11 pKa = 4.11RR12 pKa = 11.84VVGTSTLTKK21 pKa = 10.33KK22 pKa = 10.35ILTTIYY28 pKa = 9.1CTLL31 pKa = 3.54

Molecular weight: 3.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

122

0

122

24854

29

1759

203.7

23.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.571 ± 0.376

0.825 ± 0.122

6.542 ± 0.243

7.785 ± 0.37

4.136 ± 0.175

5.701 ± 0.459

1.811 ± 0.103

7.854 ± 0.231

9.532 ± 0.272

8.152 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.125

6.792 ± 0.153

2.189 ± 0.117

3.621 ± 0.15

4.201 ± 0.143

6.257 ± 0.161

5.335 ± 0.167

5.681 ± 0.18

1.09 ± 0.189

5.227 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski