Amino acid dipepetide frequency for Achromobacter phage JWDelta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.077AlaAla: 11.077 ± 1.225
0.476AlaCys: 0.476 ± 0.168
4.76AlaAsp: 4.76 ± 0.499
6.317AlaGlu: 6.317 ± 0.892
2.769AlaPhe: 2.769 ± 0.381
6.274AlaGly: 6.274 ± 0.789
1.731AlaHis: 1.731 ± 0.332
4.803AlaIle: 4.803 ± 0.405
5.063AlaLys: 5.063 ± 0.619
9.0AlaLeu: 9.0 ± 1.312
3.115AlaMet: 3.115 ± 0.358
4.76AlaAsn: 4.76 ± 0.45
4.37AlaPro: 4.37 ± 0.619
4.457AlaGln: 4.457 ± 0.727
4.327AlaArg: 4.327 ± 0.406
4.716AlaSer: 4.716 ± 0.489
5.625AlaThr: 5.625 ± 0.628
6.75AlaVal: 6.75 ± 0.759
1.212AlaTrp: 1.212 ± 0.219
3.115AlaTyr: 3.115 ± 0.317
0.0AlaXaa: 0.0 ± 0.0
Cys
0.563CysAla: 0.563 ± 0.208
0.173CysCys: 0.173 ± 0.093
0.476CysAsp: 0.476 ± 0.155
0.476CysGlu: 0.476 ± 0.17
0.346CysPhe: 0.346 ± 0.133
0.909CysGly: 0.909 ± 0.274
0.216CysHis: 0.216 ± 0.102
0.563CysIle: 0.563 ± 0.185
0.779CysLys: 0.779 ± 0.207
0.736CysLeu: 0.736 ± 0.244
0.26CysMet: 0.26 ± 0.12
0.519CysAsn: 0.519 ± 0.163
0.519CysPro: 0.519 ± 0.173
0.173CysGln: 0.173 ± 0.082
0.26CysArg: 0.26 ± 0.119
0.389CysSer: 0.389 ± 0.154
0.606CysThr: 0.606 ± 0.184
0.519CysVal: 0.519 ± 0.169
0.216CysTrp: 0.216 ± 0.112
0.087CysTyr: 0.087 ± 0.068
0.0CysXaa: 0.0 ± 0.0
Asp
6.62AspAla: 6.62 ± 0.664
0.519AspCys: 0.519 ± 0.171
2.856AspAsp: 2.856 ± 0.418
3.808AspGlu: 3.808 ± 0.408
2.466AspPhe: 2.466 ± 0.302
4.413AspGly: 4.413 ± 0.536
1.558AspHis: 1.558 ± 0.259
2.899AspIle: 2.899 ± 0.449
3.029AspLys: 3.029 ± 0.377
5.192AspLeu: 5.192 ± 0.458
2.12AspMet: 2.12 ± 0.298
2.423AspAsn: 2.423 ± 0.34
3.635AspPro: 3.635 ± 0.38
2.639AspGln: 2.639 ± 0.293
3.635AspArg: 3.635 ± 0.403
2.769AspSer: 2.769 ± 0.303
3.072AspThr: 3.072 ± 0.336
3.764AspVal: 3.764 ± 0.35
0.865AspTrp: 0.865 ± 0.222
1.774AspTyr: 1.774 ± 0.338
0.0AspXaa: 0.0 ± 0.0
Glu
6.447GluAla: 6.447 ± 0.919
0.476GluCys: 0.476 ± 0.171
3.202GluAsp: 3.202 ± 0.401
4.673GluGlu: 4.673 ± 0.679
1.861GluPhe: 1.861 ± 0.344
4.024GluGly: 4.024 ± 0.579
1.298GluHis: 1.298 ± 0.267
3.505GluIle: 3.505 ± 0.488
3.245GluLys: 3.245 ± 0.386
5.192GluLeu: 5.192 ± 0.594
1.99GluMet: 1.99 ± 0.221
2.25GluAsn: 2.25 ± 0.333
1.861GluPro: 1.861 ± 0.39
2.899GluGln: 2.899 ± 0.363
3.029GluArg: 3.029 ± 0.323
3.202GluSer: 3.202 ± 0.349
2.769GluThr: 2.769 ± 0.273
4.413GluVal: 4.413 ± 0.484
0.822GluTrp: 0.822 ± 0.177
1.904GluTyr: 1.904 ± 0.253
0.0GluXaa: 0.0 ± 0.0
Phe
2.553PheAla: 2.553 ± 0.366
0.303PheCys: 0.303 ± 0.119
2.726PheAsp: 2.726 ± 0.272
1.125PheGlu: 1.125 ± 0.204
1.471PhePhe: 1.471 ± 0.213
3.115PheGly: 3.115 ± 0.396
0.606PheHis: 0.606 ± 0.197
1.904PheIle: 1.904 ± 0.284
2.034PheLys: 2.034 ± 0.336
2.813PheLeu: 2.813 ± 0.404
1.514PheMet: 1.514 ± 0.273
1.774PheAsn: 1.774 ± 0.333
1.298PhePro: 1.298 ± 0.296
1.125PheGln: 1.125 ± 0.216
2.207PheArg: 2.207 ± 0.336
2.466PheSer: 2.466 ± 0.349
2.034PheThr: 2.034 ± 0.304
2.034PheVal: 2.034 ± 0.296
0.476PheTrp: 0.476 ± 0.132
1.168PheTyr: 1.168 ± 0.223
0.0PheXaa: 0.0 ± 0.0
Gly
5.668GlyAla: 5.668 ± 0.627
0.779GlyCys: 0.779 ± 0.215
3.981GlyAsp: 3.981 ± 0.475
3.894GlyGlu: 3.894 ± 0.392
2.856GlyPhe: 2.856 ± 0.342
5.495GlyGly: 5.495 ± 0.963
0.995GlyHis: 0.995 ± 0.219
4.067GlyIle: 4.067 ± 0.509
4.846GlyLys: 4.846 ± 0.5
5.192GlyLeu: 5.192 ± 0.549
2.899GlyMet: 2.899 ± 0.388
4.197GlyAsn: 4.197 ± 0.556
2.38GlyPro: 2.38 ± 0.354
2.856GlyGln: 2.856 ± 0.428
3.462GlyArg: 3.462 ± 0.442
4.976GlySer: 4.976 ± 0.501
4.803GlyThr: 4.803 ± 0.468
5.582GlyVal: 5.582 ± 0.473
1.168GlyTrp: 1.168 ± 0.236
2.51GlyTyr: 2.51 ± 0.272
0.0GlyXaa: 0.0 ± 0.0
His
1.861HisAla: 1.861 ± 0.274
0.346HisCys: 0.346 ± 0.14
1.558HisAsp: 1.558 ± 0.257
0.909HisGlu: 0.909 ± 0.187
1.038HisPhe: 1.038 ± 0.254
1.168HisGly: 1.168 ± 0.34
0.433HisHis: 0.433 ± 0.167
1.125HisIle: 1.125 ± 0.263
1.471HisLys: 1.471 ± 0.27
2.337HisLeu: 2.337 ± 0.332
0.692HisMet: 0.692 ± 0.173
1.082HisAsn: 1.082 ± 0.168
1.514HisPro: 1.514 ± 0.312
0.692HisGln: 0.692 ± 0.168
1.688HisArg: 1.688 ± 0.312
0.909HisSer: 0.909 ± 0.198
0.909HisThr: 0.909 ± 0.222
1.341HisVal: 1.341 ± 0.305
0.433HisTrp: 0.433 ± 0.124
0.736HisTyr: 0.736 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
4.716IleAla: 4.716 ± 0.464
0.303IleCys: 0.303 ± 0.113
3.808IleAsp: 3.808 ± 0.405
2.899IleGlu: 2.899 ± 0.397
1.601IlePhe: 1.601 ± 0.296
3.202IleGly: 3.202 ± 0.332
1.341IleHis: 1.341 ± 0.221
2.423IleIle: 2.423 ± 0.487
3.115IleLys: 3.115 ± 0.371
3.764IleLeu: 3.764 ± 0.42
1.428IleMet: 1.428 ± 0.263
2.12IleAsn: 2.12 ± 0.321
2.856IlePro: 2.856 ± 0.431
2.683IleGln: 2.683 ± 0.324
2.726IleArg: 2.726 ± 0.351
2.813IleSer: 2.813 ± 0.4
4.067IleThr: 4.067 ± 0.366
3.115IleVal: 3.115 ± 0.512
0.779IleTrp: 0.779 ± 0.206
1.688IleTyr: 1.688 ± 0.258
0.0IleXaa: 0.0 ± 0.0
Lys
5.409LysAla: 5.409 ± 0.599
0.216LysCys: 0.216 ± 0.126
4.111LysAsp: 4.111 ± 0.491
3.462LysGlu: 3.462 ± 0.401
2.034LysPhe: 2.034 ± 0.306
3.635LysGly: 3.635 ± 0.355
1.558LysHis: 1.558 ± 0.342
2.12LysIle: 2.12 ± 0.264
3.245LysLys: 3.245 ± 0.445
5.106LysLeu: 5.106 ± 0.431
2.25LysMet: 2.25 ± 0.381
2.466LysAsn: 2.466 ± 0.307
2.942LysPro: 2.942 ± 0.468
3.288LysGln: 3.288 ± 0.32
2.942LysArg: 2.942 ± 0.34
2.899LysSer: 2.899 ± 0.396
4.63LysThr: 4.63 ± 0.425
3.678LysVal: 3.678 ± 0.419
0.649LysTrp: 0.649 ± 0.197
1.385LysTyr: 1.385 ± 0.259
0.0LysXaa: 0.0 ± 0.0
Leu
7.832LeuAla: 7.832 ± 0.871
0.736LeuCys: 0.736 ± 0.194
6.144LeuAsp: 6.144 ± 0.46
4.37LeuGlu: 4.37 ± 0.373
2.942LeuPhe: 2.942 ± 0.298
6.101LeuGly: 6.101 ± 0.622
2.207LeuHis: 2.207 ± 0.354
3.288LeuIle: 3.288 ± 0.306
4.933LeuLys: 4.933 ± 0.446
6.231LeuLeu: 6.231 ± 0.662
2.726LeuMet: 2.726 ± 0.301
4.37LeuAsn: 4.37 ± 0.451
3.721LeuPro: 3.721 ± 0.341
3.635LeuGln: 3.635 ± 0.5
3.894LeuArg: 3.894 ± 0.456
4.37LeuSer: 4.37 ± 0.466
5.149LeuThr: 5.149 ± 0.491
5.928LeuVal: 5.928 ± 0.54
0.692LeuTrp: 0.692 ± 0.19
2.38LeuTyr: 2.38 ± 0.327
0.0LeuXaa: 0.0 ± 0.0
Met
3.245MetAla: 3.245 ± 0.392
0.303MetCys: 0.303 ± 0.133
1.817MetAsp: 1.817 ± 0.325
1.644MetGlu: 1.644 ± 0.198
0.822MetPhe: 0.822 ± 0.191
2.034MetGly: 2.034 ± 0.291
0.649MetHis: 0.649 ± 0.243
1.904MetIle: 1.904 ± 0.266
2.293MetLys: 2.293 ± 0.347
2.596MetLeu: 2.596 ± 0.365
0.736MetMet: 0.736 ± 0.243
1.514MetAsn: 1.514 ± 0.216
1.428MetPro: 1.428 ± 0.226
1.99MetGln: 1.99 ± 0.246
1.212MetArg: 1.212 ± 0.236
3.072MetSer: 3.072 ± 0.349
2.769MetThr: 2.769 ± 0.359
1.947MetVal: 1.947 ± 0.311
0.303MetTrp: 0.303 ± 0.153
0.822MetTyr: 0.822 ± 0.206
0.0MetXaa: 0.0 ± 0.0
Asn
3.375AsnAla: 3.375 ± 0.392
0.389AsnCys: 0.389 ± 0.15
2.207AsnAsp: 2.207 ± 0.275
2.553AsnGlu: 2.553 ± 0.315
1.428AsnPhe: 1.428 ± 0.257
3.462AsnGly: 3.462 ± 0.472
1.168AsnHis: 1.168 ± 0.19
2.077AsnIle: 2.077 ± 0.441
2.683AsnLys: 2.683 ± 0.349
4.067AsnLeu: 4.067 ± 0.414
1.125AsnMet: 1.125 ± 0.226
3.029AsnAsn: 3.029 ± 0.688
3.115AsnPro: 3.115 ± 0.346
3.029AsnGln: 3.029 ± 0.388
2.51AsnArg: 2.51 ± 0.357
3.245AsnSer: 3.245 ± 0.471
2.899AsnThr: 2.899 ± 0.458
3.072AsnVal: 3.072 ± 0.391
0.779AsnTrp: 0.779 ± 0.235
1.558AsnTyr: 1.558 ± 0.273
0.0AsnXaa: 0.0 ± 0.0
Pro
4.543ProAla: 4.543 ± 0.617
0.476ProCys: 0.476 ± 0.152
2.596ProAsp: 2.596 ± 0.442
3.678ProGlu: 3.678 ± 0.517
1.947ProPhe: 1.947 ± 0.313
3.721ProGly: 3.721 ± 0.473
0.606ProHis: 0.606 ± 0.193
2.163ProIle: 2.163 ± 0.295
2.726ProLys: 2.726 ± 0.332
2.986ProLeu: 2.986 ± 0.361
1.125ProMet: 1.125 ± 0.204
1.428ProAsn: 1.428 ± 0.262
1.601ProPro: 1.601 ± 0.375
1.644ProGln: 1.644 ± 0.3
1.644ProArg: 1.644 ± 0.336
2.769ProSer: 2.769 ± 0.306
3.721ProThr: 3.721 ± 0.355
4.587ProVal: 4.587 ± 0.544
0.649ProTrp: 0.649 ± 0.171
0.865ProTyr: 0.865 ± 0.189
0.0ProXaa: 0.0 ± 0.0
Gln
6.058GlnAla: 6.058 ± 0.868
0.216GlnCys: 0.216 ± 0.105
2.293GlnAsp: 2.293 ± 0.365
2.639GlnGlu: 2.639 ± 0.484
1.644GlnPhe: 1.644 ± 0.24
3.938GlnGly: 3.938 ± 0.462
0.909GlnHis: 0.909 ± 0.192
2.639GlnIle: 2.639 ± 0.347
2.337GlnLys: 2.337 ± 0.347
3.418GlnLeu: 3.418 ± 0.35
1.688GlnMet: 1.688 ± 0.277
2.12GlnAsn: 2.12 ± 0.356
1.212GlnPro: 1.212 ± 0.256
2.942GlnGln: 2.942 ± 0.43
2.726GlnArg: 2.726 ± 0.382
3.072GlnSer: 3.072 ± 0.502
1.99GlnThr: 1.99 ± 0.332
2.856GlnVal: 2.856 ± 0.291
0.606GlnTrp: 0.606 ± 0.139
1.688GlnTyr: 1.688 ± 0.367
0.0GlnXaa: 0.0 ± 0.0
Arg
4.543ArgAla: 4.543 ± 0.638
0.692ArgCys: 0.692 ± 0.254
3.202ArgAsp: 3.202 ± 0.422
3.115ArgGlu: 3.115 ± 0.414
2.077ArgPhe: 2.077 ± 0.331
3.159ArgGly: 3.159 ± 0.385
1.082ArgHis: 1.082 ± 0.286
3.202ArgIle: 3.202 ± 0.41
2.942ArgLys: 2.942 ± 0.404
5.106ArgLeu: 5.106 ± 0.42
2.163ArgMet: 2.163 ± 0.316
1.904ArgAsn: 1.904 ± 0.256
2.077ArgPro: 2.077 ± 0.284
2.596ArgGln: 2.596 ± 0.497
1.861ArgArg: 1.861 ± 0.393
3.288ArgSer: 3.288 ± 0.425
2.51ArgThr: 2.51 ± 0.301
3.029ArgVal: 3.029 ± 0.27
0.736ArgTrp: 0.736 ± 0.192
1.385ArgTyr: 1.385 ± 0.219
0.0ArgXaa: 0.0 ± 0.0
Ser
5.063SerAla: 5.063 ± 0.504
0.692SerCys: 0.692 ± 0.218
2.899SerAsp: 2.899 ± 0.374
3.029SerGlu: 3.029 ± 0.352
1.947SerPhe: 1.947 ± 0.3
4.37SerGly: 4.37 ± 0.526
1.341SerHis: 1.341 ± 0.322
3.072SerIle: 3.072 ± 0.363
3.548SerLys: 3.548 ± 0.346
5.365SerLeu: 5.365 ± 0.495
1.817SerMet: 1.817 ± 0.303
3.332SerAsn: 3.332 ± 0.394
2.466SerPro: 2.466 ± 0.307
2.423SerGln: 2.423 ± 0.399
3.332SerArg: 3.332 ± 0.433
3.505SerSer: 3.505 ± 0.392
4.197SerThr: 4.197 ± 0.499
4.37SerVal: 4.37 ± 0.556
0.433SerTrp: 0.433 ± 0.149
1.774SerTyr: 1.774 ± 0.311
0.0SerXaa: 0.0 ± 0.0
Thr
5.928ThrAla: 5.928 ± 0.505
0.563ThrCys: 0.563 ± 0.206
4.284ThrAsp: 4.284 ± 0.478
3.548ThrGlu: 3.548 ± 0.348
2.207ThrPhe: 2.207 ± 0.264
4.933ThrGly: 4.933 ± 0.473
0.995ThrHis: 0.995 ± 0.227
3.418ThrIle: 3.418 ± 0.424
3.029ThrLys: 3.029 ± 0.373
4.37ThrLeu: 4.37 ± 0.412
1.99ThrMet: 1.99 ± 0.256
2.813ThrAsn: 2.813 ± 0.463
3.202ThrPro: 3.202 ± 0.52
2.38ThrGln: 2.38 ± 0.326
3.288ThrArg: 3.288 ± 0.317
3.288ThrSer: 3.288 ± 0.41
4.197ThrThr: 4.197 ± 0.661
4.327ThrVal: 4.327 ± 0.514
0.995ThrTrp: 0.995 ± 0.227
1.644ThrTyr: 1.644 ± 0.308
0.0ThrXaa: 0.0 ± 0.0
Val
6.188ValAla: 6.188 ± 0.742
0.649ValCys: 0.649 ± 0.22
3.808ValAsp: 3.808 ± 0.423
4.37ValGlu: 4.37 ± 0.336
1.99ValPhe: 1.99 ± 0.307
5.279ValGly: 5.279 ± 0.573
1.99ValHis: 1.99 ± 0.35
3.635ValIle: 3.635 ± 0.517
3.851ValLys: 3.851 ± 0.343
5.192ValLeu: 5.192 ± 0.504
2.12ValMet: 2.12 ± 0.271
3.548ValAsn: 3.548 ± 0.441
3.721ValPro: 3.721 ± 0.574
3.072ValGln: 3.072 ± 0.497
3.678ValArg: 3.678 ± 0.325
4.716ValSer: 4.716 ± 0.358
3.851ValThr: 3.851 ± 0.452
4.24ValVal: 4.24 ± 0.527
0.909ValTrp: 0.909 ± 0.191
2.034ValTyr: 2.034 ± 0.255
0.0ValXaa: 0.0 ± 0.0
Trp
1.168TrpAla: 1.168 ± 0.247
0.173TrpCys: 0.173 ± 0.087
1.255TrpAsp: 1.255 ± 0.287
0.692TrpGlu: 0.692 ± 0.198
0.563TrpPhe: 0.563 ± 0.181
0.865TrpGly: 0.865 ± 0.155
0.476TrpHis: 0.476 ± 0.139
0.909TrpIle: 0.909 ± 0.178
1.038TrpLys: 1.038 ± 0.191
0.909TrpLeu: 0.909 ± 0.211
0.433TrpMet: 0.433 ± 0.128
0.606TrpAsn: 0.606 ± 0.197
0.389TrpPro: 0.389 ± 0.124
0.389TrpGln: 0.389 ± 0.124
0.649TrpArg: 0.649 ± 0.133
0.779TrpSer: 0.779 ± 0.179
0.433TrpThr: 0.433 ± 0.122
0.995TrpVal: 0.995 ± 0.207
0.26TrpTrp: 0.26 ± 0.138
0.433TrpTyr: 0.433 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.861TyrAla: 1.861 ± 0.311
0.303TyrCys: 0.303 ± 0.124
2.077TyrAsp: 2.077 ± 0.236
1.861TyrGlu: 1.861 ± 0.315
0.736TyrPhe: 0.736 ± 0.216
2.51TyrGly: 2.51 ± 0.434
1.082TyrHis: 1.082 ± 0.274
1.731TyrIle: 1.731 ± 0.402
1.817TyrLys: 1.817 ± 0.294
2.077TyrLeu: 2.077 ± 0.392
0.865TyrMet: 0.865 ± 0.18
1.514TyrAsn: 1.514 ± 0.205
1.212TyrPro: 1.212 ± 0.269
2.077TyrGln: 2.077 ± 0.329
1.601TyrArg: 1.601 ± 0.256
1.731TyrSer: 1.731 ± 0.276
1.168TyrThr: 1.168 ± 0.242
2.293TyrVal: 2.293 ± 0.357
0.433TyrTrp: 0.433 ± 0.116
1.082TyrTyr: 1.082 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (23112 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski