Gordonia phage GMA6
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K0NKV9|A0A0K0NKV9_9CAUD HNHc domain-containing protein OS=Gordonia phage GMA6 OX=1647285 GN=GMA6_89 PE=4 SV=1
MM1 pKa = 8.03 GEE3 pKa = 4.11 YY4 pKa = 10.03 TCFNCRR10 pKa = 11.84 KK11 pKa = 10.27 GIDD14 pKa = 3.19 WDD16 pKa = 3.84 QYY18 pKa = 8.81 SEE20 pKa = 4.15 EE21 pKa = 4.25 WFHH24 pKa = 8.18 LEE26 pKa = 4.9 SEE28 pKa = 4.36 QSACNSDD35 pKa = 4.09 DD36 pKa = 3.92 SPGTSAEE43 pKa = 4.08 PDD45 pKa = 3.04 RR46 pKa = 11.84 DD47 pKa = 3.56
Molecular weight: 5.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.948
IPC_protein 3.821
Toseland 3.643
ProMoST 3.897
Dawson 3.821
Bjellqvist 4.05
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.948
Patrickios 0.693
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.925
Protein with the highest isoelectric point:
>tr|A0A0K0NLA6|A0A0K0NLA6_9CAUD Uncharacterized protein OS=Gordonia phage GMA6 OX=1647285 GN=GMA6_56 PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 KK3 pKa = 8.81 HH4 pKa = 5.48 VHH6 pKa = 6.24 PAPHH10 pKa = 6.13 LVVITEE16 pKa = 4.04 DD17 pKa = 3.56 TKK19 pKa = 11.35 EE20 pKa = 3.95 GLAMLSRR27 pKa = 11.84 KK28 pKa = 8.98 IVLPVCDD35 pKa = 3.33 RR36 pKa = 11.84 RR37 pKa = 11.84 LVEE40 pKa = 3.94 RR41 pKa = 11.84 QRR43 pKa = 11.84 AQWRR47 pKa = 11.84 RR48 pKa = 11.84 AWTTGPEE55 pKa = 3.84 RR56 pKa = 11.84 GPKK59 pKa = 9.54 YY60 pKa = 10.17 EE61 pKa = 4.13 YY62 pKa = 9.94 PCWVCGEE69 pKa = 4.19 LGEE72 pKa = 4.74 IGVAGFGTKK81 pKa = 8.61 GTLIRR86 pKa = 11.84 PRR88 pKa = 11.84 NFQHH92 pKa = 6.66 ADD94 pKa = 3.16 CYY96 pKa = 11.08 EE97 pKa = 4.22 SVDD100 pKa = 4.27 EE101 pKa = 4.07 YY102 pKa = 11.64 QKK104 pKa = 10.38 MIWRR108 pKa = 11.84 IRR110 pKa = 11.84 DD111 pKa = 3.51 TKK113 pKa = 10.7 HH114 pKa = 4.81 VLRR117 pKa = 11.84 RR118 pKa = 11.84 AQRR121 pKa = 11.84 RR122 pKa = 11.84 KK123 pKa = 9.91 SKK125 pKa = 10.49 RR126 pKa = 11.84 SYY128 pKa = 10.71 DD129 pKa = 3.63 RR130 pKa = 11.84 EE131 pKa = 3.86 ALRR134 pKa = 11.84 QRR136 pKa = 11.84 RR137 pKa = 11.84 YY138 pKa = 10.96 DD139 pKa = 3.61 LGLDD143 pKa = 3.32 TSTGKK148 pKa = 9.15 QRR150 pKa = 11.84 KK151 pKa = 8.46 ARR153 pKa = 3.8
Molecular weight: 18.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.589
IPC_protein 10.233
Toseland 10.511
ProMoST 10.306
Dawson 10.643
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 10.877
Grimsley 10.687
Solomon 10.716
Lehninger 10.687
Nozaki 10.511
DTASelect 10.321
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.584
IPC_peptide 10.716
IPC2_peptide 9.37
IPC2.peptide.svr19 8.609
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
115
0
115
25834
41
1139
224.6
24.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.147 ± 0.27
0.964 ± 0.097
6.557 ± 0.251
6.329 ± 0.325
2.961 ± 0.139
8.473 ± 0.283
1.695 ± 0.127
4.568 ± 0.211
5.489 ± 0.221
7.366 ± 0.155
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.597 ± 0.147
4.088 ± 0.153
4.672 ± 0.187
3.476 ± 0.104
6.511 ± 0.239
6.375 ± 0.187
6.685 ± 0.23
7.269 ± 0.2
1.645 ± 0.134
3.132 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here