Mycobacterium lepraemurium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium avium complex (MAC)

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A250BLR8|A0A250BLR8_MYCLP Uncharacterized protein OS=Mycobacterium lepraemurium OX=64667 GN=MLM_0077 PE=4 SV=1
MM1 pKa = 7.87ADD3 pKa = 4.58DD4 pKa = 5.25KK5 pKa = 11.41DD6 pKa = 3.86ASSPGTEE13 pKa = 3.6AFVPDD18 pKa = 5.61FSDD21 pKa = 5.39DD22 pKa = 4.15DD23 pKa = 4.28DD24 pKa = 4.93AADD27 pKa = 4.06TGTQSWAPDD36 pKa = 3.43FDD38 pKa = 5.26DD39 pKa = 5.34AAEE42 pKa = 4.82DD43 pKa = 4.41DD44 pKa = 3.99DD45 pKa = 4.36TEE47 pKa = 4.17AAEE50 pKa = 4.57RR51 pKa = 11.84EE52 pKa = 4.39PEE54 pKa = 4.23PEE56 pKa = 4.38PEE58 pKa = 4.68PKK60 pKa = 10.14PEE62 pKa = 4.07PAGLAGPVPPVAVPGRR78 pKa = 11.84YY79 pKa = 8.7FYY81 pKa = 11.14VKK83 pKa = 8.04WWKK86 pKa = 10.38LVLVLLAVWAASAVVGLGLFSWWYY110 pKa = 10.39HH111 pKa = 6.49SIDD114 pKa = 3.35KK115 pKa = 10.06TPALFAVLVFVVVCVVGGVMLAMVEE140 pKa = 3.95ARR142 pKa = 11.84PLISALAVAVLFGPFAALAAAVPLYY167 pKa = 10.59GYY169 pKa = 8.11YY170 pKa = 8.65YY171 pKa = 9.38CARR174 pKa = 11.84VGHH177 pKa = 6.26CLVGVVPYY185 pKa = 10.88

Molecular weight: 19.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A250BV84|A0A250BV84_MYCLP 4HBT domain-containing protein OS=Mycobacterium lepraemurium OX=64667 GN=MLM_3940 PE=4 SV=1
MM1 pKa = 7.54GKK3 pKa = 10.2GKK5 pKa = 8.75RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.55GRR42 pKa = 11.84RR43 pKa = 11.84ALSAA47 pKa = 3.69

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2680

0

2680

840175

42

4168

313.5

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.125 ± 0.067

0.86 ± 0.015

6.13 ± 0.032

5.129 ± 0.042

2.919 ± 0.024

8.938 ± 0.045

2.267 ± 0.018

4.159 ± 0.031

2.312 ± 0.032

9.866 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.021

2.222 ± 0.022

6.021 ± 0.042

3.092 ± 0.025

7.584 ± 0.048

5.305 ± 0.029

5.78 ± 0.03

8.7 ± 0.038

1.468 ± 0.021

2.119 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski