Acipenser ruthenus (Sterlet sturgeon)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Chondrostei; Acipenseriformes; Acipenseroidei; Acipenseridae; Acipenserinae; Acipenserini; Acipenser

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A444UUG6|A0A444UUG6_ACIRT NF-kappa-B inhibitor zeta OS=Acipenser ruthenus OX=7906 GN=EOD39_20792 PE=4 SV=1
MM1 pKa = 7.13YY2 pKa = 10.76VFDD5 pKa = 5.51IDD7 pKa = 4.86LKK9 pKa = 10.98SSTDD13 pKa = 3.54VAPLVCSDD21 pKa = 3.78VNEE24 pKa = 4.82CEE26 pKa = 4.16DD27 pKa = 3.89GRR29 pKa = 11.84NGGCVANSICSNTQGSFRR47 pKa = 11.84CGPCKK52 pKa = 10.1SGYY55 pKa = 10.12IGDD58 pKa = 3.72QRR60 pKa = 11.84QGCRR64 pKa = 11.84AKK66 pKa = 10.26QGCVNGQPSPCHH78 pKa = 6.62RR79 pKa = 11.84NAQCIPQRR87 pKa = 11.84DD88 pKa = 3.7GAVTCTCGVGWAGNGFMCGEE108 pKa = 4.4DD109 pKa = 3.35TDD111 pKa = 4.57IDD113 pKa = 3.96GFPDD117 pKa = 3.36EE118 pKa = 5.53KK119 pKa = 10.83LPCPEE124 pKa = 4.58TPCQKK129 pKa = 10.68DD130 pKa = 3.04NCLTVPNSGQEE141 pKa = 4.12DD142 pKa = 4.09ADD144 pKa = 3.68SDD146 pKa = 4.74GIGDD150 pKa = 4.47ACDD153 pKa = 3.53EE154 pKa = 4.74DD155 pKa = 5.69ADD157 pKa = 4.52GDD159 pKa = 4.51GILNMEE165 pKa = 4.99DD166 pKa = 3.17NCVLVPNVNQRR177 pKa = 11.84NVDD180 pKa = 3.13QDD182 pKa = 3.92VFGDD186 pKa = 3.45ACDD189 pKa = 3.48NCRR192 pKa = 11.84LINNNDD198 pKa = 3.19QRR200 pKa = 11.84DD201 pKa = 3.5TDD203 pKa = 4.0GDD205 pKa = 4.29SKK207 pKa = 11.76GDD209 pKa = 3.63ACDD212 pKa = 4.97DD213 pKa = 5.26DD214 pKa = 5.12IDD216 pKa = 5.99GDD218 pKa = 4.37GIRR221 pKa = 11.84NALDD225 pKa = 3.29NCEE228 pKa = 4.45KK229 pKa = 10.8VPNTDD234 pKa = 2.86QRR236 pKa = 11.84DD237 pKa = 3.52RR238 pKa = 11.84DD239 pKa = 3.6GDD241 pKa = 4.25GVGDD245 pKa = 4.36ACDD248 pKa = 3.45SCPDD252 pKa = 4.21DD253 pKa = 5.29RR254 pKa = 11.84NPDD257 pKa = 3.52QVGTTIYY264 pKa = 10.42KK265 pKa = 9.56HH266 pKa = 6.0PCVLYY271 pKa = 10.74HH272 pKa = 7.08LFNPWIIHH280 pKa = 6.99DD281 pKa = 4.26GDD283 pKa = 3.6GHH285 pKa = 6.51QDD287 pKa = 3.21SRR289 pKa = 11.84DD290 pKa = 3.54NCPTVINSSQLDD302 pKa = 3.55TDD304 pKa = 3.83RR305 pKa = 11.84DD306 pKa = 4.04GLGDD310 pKa = 3.86EE311 pKa = 5.62CDD313 pKa = 5.01DD314 pKa = 5.7DD315 pKa = 7.06DD316 pKa = 7.33DD317 pKa = 7.18GDD319 pKa = 4.76GIPDD323 pKa = 4.19EE324 pKa = 5.35LPPGPDD330 pKa = 3.02NCRR333 pKa = 11.84LIPNPRR339 pKa = 11.84QEE341 pKa = 5.43DD342 pKa = 3.67SDD344 pKa = 4.64GNNPWAA350 pKa = 4.68

Molecular weight: 37.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A444USL8|A0A444USL8_ACIRT Leucine-rich repeat-containing protein 75A OS=Acipenser ruthenus OX=7906 GN=EOD39_21464 PE=4 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84NPMQSNPMQCNPMQRR18 pKa = 11.84NPMQSNPMQSNPMQHH33 pKa = 6.51NPMQRR38 pKa = 11.84NPMQHH43 pKa = 6.45NPMQRR48 pKa = 11.84NPMQRR53 pKa = 11.84NPMQRR58 pKa = 11.84NPMQRR63 pKa = 11.84NPMQRR68 pKa = 11.84NPMQRR73 pKa = 11.84NPMQRR78 pKa = 11.84NPMQRR83 pKa = 11.84NPMQRR88 pKa = 11.84NPMQRR93 pKa = 11.84NPMQRR98 pKa = 11.84NPMQRR103 pKa = 11.84NPMQRR108 pKa = 11.84NPMQRR113 pKa = 11.84NPMQRR118 pKa = 11.84NPMQRR123 pKa = 11.84NPMQRR128 pKa = 11.84NPMQRR133 pKa = 11.84NPMQRR138 pKa = 11.84NPMQRR143 pKa = 11.84NPMQRR148 pKa = 11.84NPMQRR153 pKa = 11.84NPMQRR158 pKa = 11.84NPMQRR163 pKa = 11.84NPMQRR168 pKa = 11.84NPMQRR173 pKa = 11.84NPMQRR178 pKa = 11.84NPMQRR183 pKa = 11.84NPMQRR188 pKa = 11.84NPMQRR193 pKa = 11.84NPMQRR198 pKa = 11.84NPMQRR203 pKa = 11.84NPMQRR208 pKa = 11.84NPMQRR213 pKa = 11.84NPMQRR218 pKa = 11.84NPMQRR223 pKa = 11.84NPMQRR228 pKa = 11.84NPMQRR233 pKa = 11.84NPMQHH238 pKa = 6.37NPMQHH243 pKa = 6.21NPMQHH248 pKa = 6.21NPMQHH253 pKa = 6.26NPMQRR258 pKa = 11.84NPMQHH263 pKa = 6.37NPMQHH268 pKa = 6.21NPMQHH273 pKa = 4.88NRR275 pKa = 11.84HH276 pKa = 5.47YY277 pKa = 10.7KK278 pKa = 9.39

Molecular weight: 34.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22004

0

22004

11239965

14

7624

510.8

57.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.459 ± 0.014

2.227 ± 0.016

5.091 ± 0.012

7.229 ± 0.025

3.586 ± 0.012

6.115 ± 0.02

2.575 ± 0.01

4.595 ± 0.015

6.071 ± 0.019

9.394 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.008

4.062 ± 0.012

5.502 ± 0.023

4.855 ± 0.018

5.367 ± 0.017

8.593 ± 0.024

5.616 ± 0.015

6.262 ± 0.015

1.115 ± 0.007

2.832 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski