Rousettus aegyptiacus adenovirus
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A344X9T9|A0A344X9T9_9ADEN E1B protein small T-antigen OS=Rousettus aegyptiacus adenovirus OX=1967296 PE=3 SV=1
MM1 pKa = 7.61 RR2 pKa = 11.84 HH3 pKa = 6.07 FSLTCLDD10 pKa = 4.92 PIYY13 pKa = 11.0 SVADD17 pKa = 3.61 DD18 pKa = 4.67 FLASLHH24 pKa = 6.32 SFSEE28 pKa = 4.44 EE29 pKa = 3.87 PDD31 pKa = 3.13 LSFYY35 pKa = 11.0 SDD37 pKa = 3.04 SAAPSTSHH45 pKa = 6.74 SLPDD49 pKa = 5.03 LVDD52 pKa = 4.48 LDD54 pKa = 4.3 LAPFVPLDD62 pKa = 3.35 NYY64 pKa = 10.85 VDD66 pKa = 4.61 FGYY69 pKa = 10.96 EE70 pKa = 3.74 SDD72 pKa = 4.3 EE73 pKa = 4.5 DD74 pKa = 4.25 FGLQTVPNLPPSVLLFCDD92 pKa = 4.27 APASFPDD99 pKa = 3.33 RR100 pKa = 11.84 TEE102 pKa = 4.15 IDD104 pKa = 3.45 LHH106 pKa = 8.45 CYY108 pKa = 10.27 EE109 pKa = 5.02 SMPSSVEE116 pKa = 3.79 STGAISPPPSAQVSSSPSDD135 pKa = 3.8 CDD137 pKa = 4.02 QHH139 pKa = 8.18 ICDD142 pKa = 5.26 PGNSPCSLCFLRR154 pKa = 11.84 LAYY157 pKa = 10.57 SGMCFSYY164 pKa = 10.2 TFSHH168 pKa = 7.15 LLCC171 pKa = 5.17
Molecular weight: 18.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.878
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.528
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.884
Patrickios 0.006
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A344X9U9|A0A344X9U9_9ADEN PX OS=Rousettus aegyptiacus adenovirus OX=1967296 PE=4 SV=1
MM1 pKa = 8.17 DD2 pKa = 5.38 NLTNPSRR9 pKa = 11.84 LIKK12 pKa = 10.68 EE13 pKa = 4.17 EE14 pKa = 3.83 MLQTFVPEE22 pKa = 4.09 VYY24 pKa = 10.48 GPEE27 pKa = 3.8 RR28 pKa = 11.84 VGKK31 pKa = 10.19 VGRR34 pKa = 11.84 KK35 pKa = 8.45 RR36 pKa = 11.84 KK37 pKa = 9.49 RR38 pKa = 11.84 DD39 pKa = 3.48 NPYY42 pKa = 9.62 VSLGSRR48 pKa = 11.84 RR49 pKa = 11.84 VAFNSPYY56 pKa = 11.09 AMPVKK61 pKa = 10.48 DD62 pKa = 4.33 MPRR65 pKa = 11.84 GAYY68 pKa = 6.92 QWRR71 pKa = 11.84 GRR73 pKa = 11.84 RR74 pKa = 11.84 VKK76 pKa = 10.53 RR77 pKa = 11.84 IVRR80 pKa = 11.84 PGTVIVQNNRR90 pKa = 11.84 RR91 pKa = 11.84 PRR93 pKa = 11.84 ATKK96 pKa = 10.08 RR97 pKa = 11.84 SSDD100 pKa = 3.83 EE101 pKa = 3.66 IFADD105 pKa = 4.57 EE106 pKa = 6.36 DD107 pKa = 3.83 ILTQAANRR115 pKa = 11.84 EE116 pKa = 4.15 GEE118 pKa = 3.94 FAYY121 pKa = 10.11 GKK123 pKa = 9.59 RR124 pKa = 11.84 AHH126 pKa = 6.82 IVQQGSKK133 pKa = 9.53 RR134 pKa = 11.84 KK135 pKa = 9.3 RR136 pKa = 11.84 YY137 pKa = 9.43 DD138 pKa = 3.19 VEE140 pKa = 4.1 EE141 pKa = 4.46 EE142 pKa = 4.02 EE143 pKa = 4.26 SSKK146 pKa = 10.99 RR147 pKa = 11.84 MRR149 pKa = 11.84 TGDD152 pKa = 4.65 FITEE156 pKa = 4.07 TLKK159 pKa = 10.89 RR160 pKa = 11.84 QAEE163 pKa = 4.18 EE164 pKa = 4.68 EE165 pKa = 3.99 IAQRR169 pKa = 11.84 PIRR172 pKa = 11.84 RR173 pKa = 11.84 AVKK176 pKa = 8.84 RR177 pKa = 11.84 RR178 pKa = 11.84 VVEE181 pKa = 4.09 EE182 pKa = 3.75 VDD184 pKa = 2.8 AGPRR188 pKa = 11.84 KK189 pKa = 9.3 RR190 pKa = 11.84 AYY192 pKa = 10.07 VALDD196 pKa = 3.39 TDD198 pKa = 3.87 NPTPALEE205 pKa = 4.4 AVTEE209 pKa = 4.04 QAIVPMNTGDD219 pKa = 4.02 EE220 pKa = 4.37 MVQATAQVLQTKK232 pKa = 9.84 RR233 pKa = 11.84 GRR235 pKa = 11.84 VTEE238 pKa = 3.94 RR239 pKa = 11.84 SEE241 pKa = 3.96 RR242 pKa = 11.84 AEE244 pKa = 4.01 SNIPVEE250 pKa = 4.31 VPIAEE255 pKa = 4.11 GRR257 pKa = 11.84 RR258 pKa = 11.84 VRR260 pKa = 11.84 GALKK264 pKa = 9.76 VRR266 pKa = 11.84 PIKK269 pKa = 10.39 RR270 pKa = 11.84 VAPGIGVRR278 pKa = 11.84 TVDD281 pKa = 3.73 LEE283 pKa = 4.03 IPLRR287 pKa = 11.84 EE288 pKa = 4.12 SVTNVSIPSEE298 pKa = 4.26 YY299 pKa = 10.58 EE300 pKa = 3.72 RR301 pKa = 11.84 MEE303 pKa = 4.16 TTVTRR308 pKa = 11.84 SVRR311 pKa = 11.84 RR312 pKa = 11.84 QPVRR316 pKa = 11.84 RR317 pKa = 11.84 TQKK320 pKa = 8.76 TYY322 pKa = 10.9 KK323 pKa = 8.64 PANSIIPAAVYY334 pKa = 10.0 HH335 pKa = 6.46 PSIQHH340 pKa = 5.9 PRR342 pKa = 11.84 ALPKK346 pKa = 9.88 KK347 pKa = 8.47 EE348 pKa = 4.07 RR349 pKa = 11.84 IIPVVRR355 pKa = 11.84 YY356 pKa = 8.91 HH357 pKa = 6.87 PSITMPRR364 pKa = 11.84 RR365 pKa = 11.84 YY366 pKa = 10.05
Molecular weight: 41.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.706
IPC_protein 10.628
Toseland 10.745
ProMoST 10.511
Dawson 10.847
Bjellqvist 10.57
Wikipedia 11.082
Rodwell 10.979
Grimsley 10.891
Solomon 10.994
Lehninger 10.95
Nozaki 10.716
DTASelect 10.57
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.672
IPC_peptide 10.994
IPC2_peptide 9.414
IPC2.peptide.svr19 8.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
2
25
8185
55
973
327.4
37.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.097 ± 0.254
1.698 ± 0.32
4.52 ± 0.309
6.158 ± 0.461
4.704 ± 0.297
5.437 ± 0.475
1.894 ± 0.141
5.767 ± 0.32
6.133 ± 0.635
9.42 ± 0.494
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.346 ± 0.146
6.182 ± 0.491
5.816 ± 0.296
4.301 ± 0.352
5.18 ± 0.77
7.221 ± 0.393
5.999 ± 0.344
5.999 ± 0.261
1.1 ± 0.148
4.032 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here