Rousettus aegyptiacus adenovirus 
Average proteome isoelectric point is 6.92 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A344X9T9|A0A344X9T9_9ADEN E1B protein  small T-antigen OS=Rousettus aegyptiacus adenovirus OX=1967296 PE=3 SV=1 
MM1 pKa = 7.61  RR2 pKa = 11.84  HH3 pKa = 6.07  FSLTCLDD10 pKa = 4.92  PIYY13 pKa = 11.0  SVADD17 pKa = 3.61  DD18 pKa = 4.67  FLASLHH24 pKa = 6.32  SFSEE28 pKa = 4.44  EE29 pKa = 3.87  PDD31 pKa = 3.13  LSFYY35 pKa = 11.0  SDD37 pKa = 3.04  SAAPSTSHH45 pKa = 6.74  SLPDD49 pKa = 5.03  LVDD52 pKa = 4.48  LDD54 pKa = 4.3  LAPFVPLDD62 pKa = 3.35  NYY64 pKa = 10.85  VDD66 pKa = 4.61  FGYY69 pKa = 10.96  EE70 pKa = 3.74  SDD72 pKa = 4.3  EE73 pKa = 4.5  DD74 pKa = 4.25  FGLQTVPNLPPSVLLFCDD92 pKa = 4.27  APASFPDD99 pKa = 3.33  RR100 pKa = 11.84  TEE102 pKa = 4.15  IDD104 pKa = 3.45  LHH106 pKa = 8.45  CYY108 pKa = 10.27  EE109 pKa = 5.02  SMPSSVEE116 pKa = 3.79  STGAISPPPSAQVSSSPSDD135 pKa = 3.8  CDD137 pKa = 4.02  QHH139 pKa = 8.18  ICDD142 pKa = 5.26  PGNSPCSLCFLRR154 pKa = 11.84  LAYY157 pKa = 10.57  SGMCFSYY164 pKa = 10.2  TFSHH168 pKa = 7.15  LLCC171 pKa = 5.17   
 Molecular weight: 18.63 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.878 
IPC2_protein 3.745 
IPC_protein 3.757 
Toseland    3.528 
ProMoST     3.935 
Dawson      3.77 
Bjellqvist  3.923 
Wikipedia   3.732 
Rodwell     3.579 
Grimsley    3.439 
Solomon     3.745 
Lehninger   3.706 
Nozaki      3.884 
DTASelect   4.151 
Thurlkill   3.592 
EMBOSS      3.732 
Sillero     3.884 
Patrickios  0.006 
IPC_peptide 3.745 
IPC2_peptide  3.859 
IPC2.peptide.svr19  3.801 
 Protein with the highest isoelectric point: 
>tr|A0A344X9U9|A0A344X9U9_9ADEN PX OS=Rousettus aegyptiacus adenovirus OX=1967296 PE=4 SV=1 
MM1 pKa = 8.17  DD2 pKa = 5.38  NLTNPSRR9 pKa = 11.84  LIKK12 pKa = 10.68  EE13 pKa = 4.17  EE14 pKa = 3.83  MLQTFVPEE22 pKa = 4.09  VYY24 pKa = 10.48  GPEE27 pKa = 3.8  RR28 pKa = 11.84  VGKK31 pKa = 10.19  VGRR34 pKa = 11.84  KK35 pKa = 8.45  RR36 pKa = 11.84  KK37 pKa = 9.49  RR38 pKa = 11.84  DD39 pKa = 3.48  NPYY42 pKa = 9.62  VSLGSRR48 pKa = 11.84  RR49 pKa = 11.84  VAFNSPYY56 pKa = 11.09  AMPVKK61 pKa = 10.48  DD62 pKa = 4.33  MPRR65 pKa = 11.84  GAYY68 pKa = 6.92  QWRR71 pKa = 11.84  GRR73 pKa = 11.84  RR74 pKa = 11.84  VKK76 pKa = 10.53  RR77 pKa = 11.84  IVRR80 pKa = 11.84  PGTVIVQNNRR90 pKa = 11.84  RR91 pKa = 11.84  PRR93 pKa = 11.84  ATKK96 pKa = 10.08  RR97 pKa = 11.84  SSDD100 pKa = 3.83  EE101 pKa = 3.66  IFADD105 pKa = 4.57  EE106 pKa = 6.36  DD107 pKa = 3.83  ILTQAANRR115 pKa = 11.84  EE116 pKa = 4.15  GEE118 pKa = 3.94  FAYY121 pKa = 10.11  GKK123 pKa = 9.59  RR124 pKa = 11.84  AHH126 pKa = 6.82  IVQQGSKK133 pKa = 9.53  RR134 pKa = 11.84  KK135 pKa = 9.3  RR136 pKa = 11.84  YY137 pKa = 9.43  DD138 pKa = 3.19  VEE140 pKa = 4.1  EE141 pKa = 4.46  EE142 pKa = 4.02  EE143 pKa = 4.26  SSKK146 pKa = 10.99  RR147 pKa = 11.84  MRR149 pKa = 11.84  TGDD152 pKa = 4.65  FITEE156 pKa = 4.07  TLKK159 pKa = 10.89  RR160 pKa = 11.84  QAEE163 pKa = 4.18  EE164 pKa = 4.68  EE165 pKa = 3.99  IAQRR169 pKa = 11.84  PIRR172 pKa = 11.84  RR173 pKa = 11.84  AVKK176 pKa = 8.84  RR177 pKa = 11.84  RR178 pKa = 11.84  VVEE181 pKa = 4.09  EE182 pKa = 3.75  VDD184 pKa = 2.8  AGPRR188 pKa = 11.84  KK189 pKa = 9.3  RR190 pKa = 11.84  AYY192 pKa = 10.07  VALDD196 pKa = 3.39  TDD198 pKa = 3.87  NPTPALEE205 pKa = 4.4  AVTEE209 pKa = 4.04  QAIVPMNTGDD219 pKa = 4.02  EE220 pKa = 4.37  MVQATAQVLQTKK232 pKa = 9.84  RR233 pKa = 11.84  GRR235 pKa = 11.84  VTEE238 pKa = 3.94  RR239 pKa = 11.84  SEE241 pKa = 3.96  RR242 pKa = 11.84  AEE244 pKa = 4.01  SNIPVEE250 pKa = 4.31  VPIAEE255 pKa = 4.11  GRR257 pKa = 11.84  RR258 pKa = 11.84  VRR260 pKa = 11.84  GALKK264 pKa = 9.76  VRR266 pKa = 11.84  PIKK269 pKa = 10.39  RR270 pKa = 11.84  VAPGIGVRR278 pKa = 11.84  TVDD281 pKa = 3.73  LEE283 pKa = 4.03  IPLRR287 pKa = 11.84  EE288 pKa = 4.12  SVTNVSIPSEE298 pKa = 4.26  YY299 pKa = 10.58  EE300 pKa = 3.72  RR301 pKa = 11.84  MEE303 pKa = 4.16  TTVTRR308 pKa = 11.84  SVRR311 pKa = 11.84  RR312 pKa = 11.84  QPVRR316 pKa = 11.84  RR317 pKa = 11.84  TQKK320 pKa = 8.76  TYY322 pKa = 10.9  KK323 pKa = 8.64  PANSIIPAAVYY334 pKa = 10.0  HH335 pKa = 6.46  PSIQHH340 pKa = 5.9  PRR342 pKa = 11.84  ALPKK346 pKa = 9.88  KK347 pKa = 8.47  EE348 pKa = 4.07  RR349 pKa = 11.84  IIPVVRR355 pKa = 11.84  YY356 pKa = 8.91  HH357 pKa = 6.87  PSITMPRR364 pKa = 11.84  RR365 pKa = 11.84  YY366 pKa = 10.05   
 Molecular weight: 41.97 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.33 
IPC2_protein 9.706 
IPC_protein 10.628 
Toseland    10.745 
ProMoST     10.511 
Dawson      10.847 
Bjellqvist  10.57 
Wikipedia   11.082 
Rodwell     10.979 
Grimsley    10.891 
Solomon     10.994 
Lehninger   10.95 
Nozaki      10.716 
DTASelect   10.57 
Thurlkill   10.745 
EMBOSS      11.155 
Sillero     10.774 
Patrickios  10.672 
IPC_peptide 10.994 
IPC2_peptide  9.414 
IPC2.peptide.svr19  8.669 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        23 
 
        
        2 
 
        
        25 
         
        8185
 
        55
 
        973
 
        327.4
 
        37.08
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        6.097 ± 0.254
1.698 ± 0.32
 
        4.52 ± 0.309
6.158 ± 0.461
 
        4.704 ± 0.297
5.437 ± 0.475
 
        1.894 ± 0.141
5.767 ± 0.32
       
        6.133 ± 0.635
9.42 ± 0.494
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.346 ± 0.146
6.182 ± 0.491
 
        5.816 ± 0.296
4.301 ± 0.352
 
        5.18 ± 0.77
7.221 ± 0.393
 
        5.999 ± 0.344
5.999 ± 0.261
       
        1.1 ± 0.148
4.032 ± 0.392
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here