Proteome-pI 2.0 - Proteome Isoelectric Point Database

Mycobacterium phage Kenuha5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A5Q2WHJ5|A0A5Q2WHJ5_9CAUD Uncharacterized protein OS=Mycobacterium phage Kenuha5 OX=2653759 GN=73 PE=4 SV=1

MM1 pKa = 7.52NNPEE5 pKa = 3.95LRR7 pKa = 11.84AVLTEE12 pKa = 3.88ALSRR16 pKa = 11.84SYY18 pKa = 11.18YY19 pKa = 10.27RR20 pKa = 11.84IVGSSSDD27 pKa = 3.53CRR29 pKa = 11.84VDD31 pKa = 3.2PGEE34 pKa = 4.22ILADD38 pKa = 5.07AILSLPGVAVIQLPEE53 pKa = 4.53PNSTRR58 pKa = 11.84YY59 pKa = 9.62EE60 pKa = 4.27DD61 pKa = 4.45EE62 pKa = 5.37DD63 pKa = 5.76DD64 pKa = 4.18EE65 pKa = 5.21FPPADD70 pKa = 4.3RR71 pKa = 11.84LAWWYY76 pKa = 9.65PGSLFGISQWGYY88 pKa = 8.12PNEE91 pKa = 3.98VQIAYY96 pKa = 9.95NGEE99 pKa = 3.78PFEE102 pKa = 4.3PVDD105 pKa = 3.7IIEE108 pKa = 4.47ARR110 pKa = 11.84FIAAALLAAAAAAVVAEE127 pKa = 4.87GEE129 pKa = 4.39DD130 pKa = 3.55KK131 pKa = 11.29

Molecular weight: 14.29 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A5Q2WJL1|A0A5Q2WJL1_9CAUD Uncharacterized protein OS=Mycobacterium phage Kenuha5 OX=2653759 GN=40 PE=4 SV=1

MM1 pKa = 7.55SSATTASSRR10 pKa = 11.84LFPHH14 pKa = 6.76SKK16 pKa = 10.31RR17 pKa = 11.84PPPCRR22 pKa = 11.84ARR24 pKa = 11.84ATDD27 pKa = 3.38NRR29 pKa = 11.84EE30 pKa = 3.89KK31 pKa = 10.45EE32 pKa = 4.28VSVSEE37 pKa = 4.09LQLTKK42 pKa = 10.74DD43 pKa = 3.44EE44 pKa = 4.19YY45 pKa = 10.97HH46 pKa = 6.87LYY48 pKa = 10.45VLEE51 pKa = 4.06LVGFGVKK58 pKa = 10.18VGISKK63 pKa = 10.28NPRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84LATLRR73 pKa = 11.84KK74 pKa = 8.57HH75 pKa = 6.63AEE77 pKa = 3.9GHH79 pKa = 4.83GTPVGRR85 pKa = 11.84AWHH88 pKa = 6.9RR89 pKa = 11.84II90 pKa = 3.55

Molecular weight: 10.14 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
96	0	96	18175	30	1211	189.3	20.71

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
10.729 ± 0.546	1.15 ± 0.159	6.564 ± 0.23	5.821 ± 0.368	2.85 ± 0.19	8.787 ± 0.674	2.327 ± 0.209	4.407 ± 0.168	3.241 ± 0.189	7.406 ± 0.239

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.283 ± 0.115	3.257 ± 0.18	5.948 ± 0.224	3.472 ± 0.156	7.131 ± 0.424	5.777 ± 0.276	6.916 ± 0.234	7.175 ± 0.271	2.272 ± 0.147	2.487 ± 0.168

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski