Pirellula staleyi (strain ATCC 27377 / DSM 6068 / ICPB 4128) (Pirella staleyi)

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Pirellula; Pirellula staleyi

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4711 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2R746|D2R746_PIRSD Uncharacterized protein OS=Pirellula staleyi (strain ATCC 27377 / DSM 6068 / ICPB 4128) OX=530564 GN=Psta_4607 PE=4 SV=1
MM1 pKa = 6.91KK2 pKa = 10.23HH3 pKa = 4.46LWKK6 pKa = 10.08WLGEE10 pKa = 3.91ALRR13 pKa = 11.84RR14 pKa = 11.84STGEE18 pKa = 3.68SSSRR22 pKa = 11.84SHH24 pKa = 6.47SRR26 pKa = 11.84SKK28 pKa = 10.66NRR30 pKa = 11.84RR31 pKa = 11.84GRR33 pKa = 11.84LRR35 pKa = 11.84LEE37 pKa = 4.13SLEE40 pKa = 4.03SRR42 pKa = 11.84AMMASDD48 pKa = 4.16ILPVLMVVADD58 pKa = 3.68QSDD61 pKa = 3.96FYY63 pKa = 9.91YY64 pKa = 10.54QEE66 pKa = 4.32YY67 pKa = 10.45GDD69 pKa = 3.47TRR71 pKa = 11.84MSIEE75 pKa = 3.88AAGLDD80 pKa = 3.68VQVAATTTNPTVPHH94 pKa = 6.16VNTGEE99 pKa = 4.16GADD102 pKa = 4.17GGLLTPDD109 pKa = 4.07LALADD114 pKa = 4.27VNASDD119 pKa = 3.8YY120 pKa = 11.09SAIVFVGGWGSSMYY134 pKa = 10.44QYY136 pKa = 11.08AFPGTYY142 pKa = 9.74SDD144 pKa = 3.31GRR146 pKa = 11.84YY147 pKa = 10.26NGDD150 pKa = 3.62LATKK154 pKa = 10.21GVVNEE159 pKa = 4.99LINDD163 pKa = 3.92FVEE166 pKa = 3.64QDD168 pKa = 3.22KK169 pKa = 11.24YY170 pKa = 9.63VTAICHH176 pKa = 5.38GTTVLAWARR185 pKa = 11.84VDD187 pKa = 4.17GVSLLAGRR195 pKa = 11.84TVAIPYY201 pKa = 9.37IGSPATFYY209 pKa = 10.29QGQYY213 pKa = 9.3YY214 pKa = 10.68GYY216 pKa = 10.69FEE218 pKa = 5.75LSQQAQMDD226 pKa = 3.98FNGAITQGSSGAIGDD241 pKa = 4.6PDD243 pKa = 3.71TAADD247 pKa = 4.19DD248 pKa = 3.63VWVDD252 pKa = 3.4GKK254 pKa = 10.88IITAEE259 pKa = 4.13NYY261 pKa = 10.28DD262 pKa = 3.69SAAMFGQVIASHH274 pKa = 6.81LLADD278 pKa = 5.05AEE280 pKa = 4.59GDD282 pKa = 4.04DD283 pKa = 4.42EE284 pKa = 5.49GGPIDD289 pKa = 5.02PPPPTNHH296 pKa = 6.88APTIADD302 pKa = 2.85GSMSIEE308 pKa = 4.17EE309 pKa = 4.07NSAIATLVVQVSGSDD324 pKa = 3.49VDD326 pKa = 4.72PEE328 pKa = 4.34DD329 pKa = 4.07LLTYY333 pKa = 10.46SIVSGNEE340 pKa = 3.4SGIFSIDD347 pKa = 3.07AATGEE352 pKa = 4.04IRR354 pKa = 11.84IASSTNLDD362 pKa = 3.69FEE364 pKa = 6.75ADD366 pKa = 3.51ALHH369 pKa = 6.01TLVVAVSDD377 pKa = 4.47GEE379 pKa = 4.3LSSEE383 pKa = 3.99ASIEE387 pKa = 4.11IAVLDD392 pKa = 3.91VDD394 pKa = 4.46EE395 pKa = 5.2TPPPPPMPLPPGPSNLTLPASPVAVVDD422 pKa = 4.07GVLIVQGTEE431 pKa = 3.85GADD434 pKa = 3.37YY435 pKa = 10.91LYY437 pKa = 10.65VYY439 pKa = 10.08PGAQAGSVMVYY450 pKa = 10.58RR451 pKa = 11.84NGQYY455 pKa = 10.67FSFAAGTVNSAVIHH469 pKa = 6.0ALGGDD474 pKa = 3.99DD475 pKa = 5.61FVGTCMLNLSVEE487 pKa = 4.26IYY489 pKa = 10.5GGNGHH494 pKa = 7.32DD495 pKa = 4.14EE496 pKa = 4.22LHH498 pKa = 6.33GAIAPSYY505 pKa = 9.7IDD507 pKa = 3.71GGEE510 pKa = 4.11GSDD513 pKa = 4.02RR514 pKa = 11.84IYY516 pKa = 11.29GGASSDD522 pKa = 3.71WIVGGNGDD530 pKa = 3.92DD531 pKa = 3.75QIEE534 pKa = 4.21ARR536 pKa = 11.84GGADD540 pKa = 3.5LVQGGAGTDD549 pKa = 3.81TINGGLGNDD558 pKa = 3.68ILIGGQGPDD567 pKa = 3.66RR568 pKa = 11.84LNGQADD574 pKa = 4.25DD575 pKa = 4.73DD576 pKa = 4.46LLIGGDD582 pKa = 3.82LQSAAVGASLLGLLAQWNTDD602 pKa = 2.84AATRR606 pKa = 11.84AQIWDD611 pKa = 3.52QGDD614 pKa = 3.41LGGDD618 pKa = 3.83FSVVGDD624 pKa = 4.12SAVDD628 pKa = 3.65CLFSGSGNDD637 pKa = 3.19WLLAGLTDD645 pKa = 4.34YY646 pKa = 10.66IYY648 pKa = 10.48QQEE651 pKa = 4.44PGDD654 pKa = 3.75EE655 pKa = 4.15LVRR658 pKa = 11.84RR659 pKa = 4.2

Molecular weight: 68.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2R1P8|D2R1P8_PIRSD Planctomycete cytochrome C OS=Pirellula staleyi (strain ATCC 27377 / DSM 6068 / ICPB 4128) OX=530564 GN=Psta_2093 PE=4 SV=1
MM1 pKa = 7.61AKK3 pKa = 9.85PHH5 pKa = 6.76RR6 pKa = 11.84SHH8 pKa = 7.16KK9 pKa = 9.8KK10 pKa = 8.31ANHH13 pKa = 5.91GKK15 pKa = 9.98RR16 pKa = 11.84PANAKK21 pKa = 9.59ARR23 pKa = 11.84RR24 pKa = 11.84AKK26 pKa = 9.74RR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 9.54VRR31 pKa = 11.84TT32 pKa = 3.48

Molecular weight: 3.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4711

0

4711

1765092

30

5010

374.7

40.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.629 ± 0.049

1.055 ± 0.015

5.441 ± 0.028

6.214 ± 0.045

3.449 ± 0.022

7.542 ± 0.056

2.062 ± 0.022

4.96 ± 0.024

4.013 ± 0.04

10.377 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.02

2.841 ± 0.04

5.583 ± 0.04

3.96 ± 0.023

6.325 ± 0.044

6.681 ± 0.036

5.794 ± 0.048

7.079 ± 0.03

1.414 ± 0.014

2.374 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski