Amino acid dipepetide frequency for Stenotrophomonas phage Smp131

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.209AlaAla: 16.209 ± 2.29
1.094AlaCys: 1.094 ± 0.368
7.657AlaAsp: 7.657 ± 0.902
5.668AlaGlu: 5.668 ± 0.694
2.983AlaPhe: 2.983 ± 0.636
11.237AlaGly: 11.237 ± 1.72
3.083AlaHis: 3.083 ± 0.461
4.972AlaIle: 4.972 ± 0.646
3.878AlaLys: 3.878 ± 0.655
10.342AlaLeu: 10.342 ± 1.154
3.978AlaMet: 3.978 ± 0.615
3.481AlaAsn: 3.481 ± 0.611
5.768AlaPro: 5.768 ± 0.726
6.066AlaGln: 6.066 ± 0.894
8.751AlaArg: 8.751 ± 1.084
8.154AlaSer: 8.154 ± 0.937
9.646AlaThr: 9.646 ± 1.421
7.558AlaVal: 7.558 ± 0.83
2.188AlaTrp: 2.188 ± 0.534
2.685AlaTyr: 2.685 ± 0.462
0.0AlaXaa: 0.0 ± 0.0
Cys
1.691CysAla: 1.691 ± 0.396
0.398CysCys: 0.398 ± 0.191
0.994CysAsp: 0.994 ± 0.373
0.597CysGlu: 0.597 ± 0.24
0.199CysPhe: 0.199 ± 0.115
1.492CysGly: 1.492 ± 0.457
0.398CysHis: 0.398 ± 0.173
0.0CysIle: 0.0 ± 0.0
0.0CysLys: 0.0 ± 0.0
0.199CysLeu: 0.199 ± 0.129
0.099CysMet: 0.099 ± 0.113
0.099CysAsn: 0.099 ± 0.113
1.293CysPro: 1.293 ± 0.366
0.398CysGln: 0.398 ± 0.212
0.796CysArg: 0.796 ± 0.291
0.696CysSer: 0.696 ± 0.242
0.497CysThr: 0.497 ± 0.239
0.994CysVal: 0.994 ± 0.316
0.099CysTrp: 0.099 ± 0.097
0.199CysTyr: 0.199 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
7.359AspAla: 7.359 ± 0.743
0.0AspCys: 0.0 ± 0.0
2.784AspAsp: 2.784 ± 0.408
2.685AspGlu: 2.685 ± 0.377
1.989AspPhe: 1.989 ± 0.366
4.773AspGly: 4.773 ± 0.852
0.994AspHis: 0.994 ± 0.268
2.387AspIle: 2.387 ± 0.545
1.492AspLys: 1.492 ± 0.362
5.27AspLeu: 5.27 ± 0.703
1.293AspMet: 1.293 ± 0.347
0.696AspAsn: 0.696 ± 0.305
2.983AspPro: 2.983 ± 0.463
1.889AspGln: 1.889 ± 0.36
3.878AspArg: 3.878 ± 0.725
2.486AspSer: 2.486 ± 0.702
3.381AspThr: 3.381 ± 0.633
4.276AspVal: 4.276 ± 0.601
0.796AspTrp: 0.796 ± 0.253
2.088AspTyr: 2.088 ± 0.493
0.0AspXaa: 0.0 ± 0.0
Glu
5.469GluAla: 5.469 ± 0.767
0.398GluCys: 0.398 ± 0.273
1.889GluAsp: 1.889 ± 0.389
1.989GluGlu: 1.989 ± 0.493
3.282GluPhe: 3.282 ± 0.694
3.083GluGly: 3.083 ± 0.59
0.895GluHis: 0.895 ± 0.269
1.989GluIle: 1.989 ± 0.491
2.188GluLys: 2.188 ± 0.572
5.37GluLeu: 5.37 ± 0.698
0.597GluMet: 0.597 ± 0.264
1.193GluAsn: 1.193 ± 0.412
2.088GluPro: 2.088 ± 0.431
2.784GluGln: 2.784 ± 0.396
4.773GluArg: 4.773 ± 0.609
3.083GluSer: 3.083 ± 0.593
2.088GluThr: 2.088 ± 0.457
3.481GluVal: 3.481 ± 0.639
1.293GluTrp: 1.293 ± 0.293
1.293GluTyr: 1.293 ± 0.313
0.0GluXaa: 0.0 ± 0.0
Phe
3.083PheAla: 3.083 ± 0.472
0.199PheCys: 0.199 ± 0.147
3.481PheAsp: 3.481 ± 0.675
1.889PheGlu: 1.889 ± 0.378
1.094PhePhe: 1.094 ± 0.401
2.287PheGly: 2.287 ± 0.492
0.696PheHis: 0.696 ± 0.295
0.994PheIle: 0.994 ± 0.27
1.492PheLys: 1.492 ± 0.376
2.287PheLeu: 2.287 ± 0.558
0.298PheMet: 0.298 ± 0.184
1.392PheAsn: 1.392 ± 0.39
1.79PhePro: 1.79 ± 0.394
0.895PheGln: 0.895 ± 0.318
1.989PheArg: 1.989 ± 0.492
1.591PheSer: 1.591 ± 0.265
2.287PheThr: 2.287 ± 0.469
1.989PheVal: 1.989 ± 0.437
0.696PheTrp: 0.696 ± 0.273
0.398PheTyr: 0.398 ± 0.249
0.0PheXaa: 0.0 ± 0.0
Gly
9.745GlyAla: 9.745 ± 0.946
0.696GlyCys: 0.696 ± 0.278
4.375GlyAsp: 4.375 ± 0.774
3.182GlyGlu: 3.182 ± 0.535
2.287GlyPhe: 2.287 ± 0.51
7.06GlyGly: 7.06 ± 1.106
1.889GlyHis: 1.889 ± 0.483
4.177GlyIle: 4.177 ± 0.784
3.282GlyLys: 3.282 ± 0.391
7.558GlyLeu: 7.558 ± 1.125
1.889GlyMet: 1.889 ± 0.402
2.983GlyAsn: 2.983 ± 0.523
3.182GlyPro: 3.182 ± 0.585
4.475GlyGln: 4.475 ± 0.946
5.967GlyArg: 5.967 ± 0.874
4.873GlySer: 4.873 ± 0.886
6.464GlyThr: 6.464 ± 0.758
5.37GlyVal: 5.37 ± 0.684
1.691GlyTrp: 1.691 ± 0.33
1.889GlyTyr: 1.889 ± 0.476
0.0GlyXaa: 0.0 ± 0.0
His
2.287HisAla: 2.287 ± 0.376
0.696HisCys: 0.696 ± 0.277
0.895HisAsp: 0.895 ± 0.348
1.392HisGlu: 1.392 ± 0.411
0.597HisPhe: 0.597 ± 0.209
2.088HisGly: 2.088 ± 0.433
0.994HisHis: 0.994 ± 0.399
0.994HisIle: 0.994 ± 0.285
1.094HisLys: 1.094 ± 0.271
1.889HisLeu: 1.889 ± 0.561
0.398HisMet: 0.398 ± 0.259
0.597HisAsn: 0.597 ± 0.243
0.895HisPro: 0.895 ± 0.332
0.895HisGln: 0.895 ± 0.31
3.083HisArg: 3.083 ± 0.697
1.392HisSer: 1.392 ± 0.452
1.193HisThr: 1.193 ± 0.345
0.994HisVal: 0.994 ± 0.393
0.099HisTrp: 0.099 ± 0.101
0.298HisTyr: 0.298 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
5.171IleAla: 5.171 ± 0.903
0.895IleCys: 0.895 ± 0.28
2.884IleAsp: 2.884 ± 0.563
3.58IleGlu: 3.58 ± 0.534
1.094IlePhe: 1.094 ± 0.344
3.58IleGly: 3.58 ± 0.597
0.895IleHis: 0.895 ± 0.328
0.994IleIle: 0.994 ± 0.39
1.392IleLys: 1.392 ± 0.424
2.486IleLeu: 2.486 ± 0.452
0.696IleMet: 0.696 ± 0.256
2.287IleAsn: 2.287 ± 0.607
1.293IlePro: 1.293 ± 0.39
1.79IleGln: 1.79 ± 0.392
2.387IleArg: 2.387 ± 0.536
2.586IleSer: 2.586 ± 0.537
2.586IleThr: 2.586 ± 0.553
2.685IleVal: 2.685 ± 0.504
0.497IleTrp: 0.497 ± 0.202
0.895IleTyr: 0.895 ± 0.28
0.0IleXaa: 0.0 ± 0.0
Lys
3.878LysAla: 3.878 ± 0.659
0.398LysCys: 0.398 ± 0.235
1.193LysAsp: 1.193 ± 0.247
1.989LysGlu: 1.989 ± 0.462
0.895LysPhe: 0.895 ± 0.269
1.989LysGly: 1.989 ± 0.402
0.597LysHis: 0.597 ± 0.269
1.392LysIle: 1.392 ± 0.432
2.287LysLys: 2.287 ± 0.595
3.481LysLeu: 3.481 ± 0.684
0.796LysMet: 0.796 ± 0.344
1.492LysAsn: 1.492 ± 0.338
1.79LysPro: 1.79 ± 0.392
0.994LysGln: 0.994 ± 0.362
2.486LysArg: 2.486 ± 0.548
1.691LysSer: 1.691 ± 0.539
2.088LysThr: 2.088 ± 0.532
1.492LysVal: 1.492 ± 0.358
0.298LysTrp: 0.298 ± 0.169
0.696LysTyr: 0.696 ± 0.296
0.0LysXaa: 0.0 ± 0.0
Leu
11.436LeuAla: 11.436 ± 1.18
0.796LeuCys: 0.796 ± 0.247
5.27LeuAsp: 5.27 ± 0.596
3.282LeuGlu: 3.282 ± 0.633
3.481LeuPhe: 3.481 ± 0.47
6.464LeuGly: 6.464 ± 1.073
2.188LeuHis: 2.188 ± 0.449
3.282LeuIle: 3.282 ± 0.471
2.486LeuLys: 2.486 ± 0.566
8.751LeuLeu: 8.751 ± 1.015
1.591LeuMet: 1.591 ± 0.324
1.989LeuAsn: 1.989 ± 0.366
5.668LeuPro: 5.668 ± 0.932
5.867LeuGln: 5.867 ± 0.697
8.154LeuArg: 8.154 ± 0.96
6.663LeuSer: 6.663 ± 0.951
5.867LeuThr: 5.867 ± 0.73
5.668LeuVal: 5.668 ± 0.869
1.094LeuTrp: 1.094 ± 0.317
1.691LeuTyr: 1.691 ± 0.475
0.0LeuXaa: 0.0 ± 0.0
Met
3.182MetAla: 3.182 ± 0.61
0.199MetCys: 0.199 ± 0.12
0.895MetAsp: 0.895 ± 0.257
1.094MetGlu: 1.094 ± 0.281
0.199MetPhe: 0.199 ± 0.137
1.79MetGly: 1.79 ± 0.441
0.298MetHis: 0.298 ± 0.19
0.597MetIle: 0.597 ± 0.233
0.696MetLys: 0.696 ± 0.32
2.784MetLeu: 2.784 ± 0.492
0.796MetMet: 0.796 ± 0.298
0.298MetAsn: 0.298 ± 0.201
1.79MetPro: 1.79 ± 0.449
1.492MetGln: 1.492 ± 0.302
1.989MetArg: 1.989 ± 0.413
1.392MetSer: 1.392 ± 0.324
1.492MetThr: 1.492 ± 0.375
1.492MetVal: 1.492 ± 0.34
0.398MetTrp: 0.398 ± 0.211
0.099MetTyr: 0.099 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
4.375AsnAla: 4.375 ± 0.786
0.199AsnCys: 0.199 ± 0.129
0.796AsnAsp: 0.796 ± 0.257
1.591AsnGlu: 1.591 ± 0.485
0.696AsnPhe: 0.696 ± 0.275
3.481AsnGly: 3.481 ± 0.837
0.199AsnHis: 0.199 ± 0.131
0.298AsnIle: 0.298 ± 0.205
0.895AsnLys: 0.895 ± 0.352
2.685AsnLeu: 2.685 ± 0.579
0.796AsnMet: 0.796 ± 0.286
0.994AsnAsn: 0.994 ± 0.283
1.691AsnPro: 1.691 ± 0.379
1.193AsnGln: 1.193 ± 0.335
1.889AsnArg: 1.889 ± 0.492
0.895AsnSer: 0.895 ± 0.288
1.591AsnThr: 1.591 ± 0.393
1.79AsnVal: 1.79 ± 0.442
0.099AsnTrp: 0.099 ± 0.091
0.597AsnTyr: 0.597 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
7.955ProAla: 7.955 ± 1.087
0.796ProCys: 0.796 ± 0.311
2.188ProAsp: 2.188 ± 0.417
4.276ProGlu: 4.276 ± 0.809
1.79ProPhe: 1.79 ± 0.37
4.375ProGly: 4.375 ± 0.628
1.79ProHis: 1.79 ± 0.663
2.188ProIle: 2.188 ± 0.411
0.696ProLys: 0.696 ± 0.266
4.475ProLeu: 4.475 ± 0.612
1.293ProMet: 1.293 ± 0.315
1.392ProAsn: 1.392 ± 0.299
2.685ProPro: 2.685 ± 0.698
1.79ProGln: 1.79 ± 0.433
3.679ProArg: 3.679 ± 0.996
3.878ProSer: 3.878 ± 0.68
3.282ProThr: 3.282 ± 0.694
3.679ProVal: 3.679 ± 0.495
0.597ProTrp: 0.597 ± 0.221
1.492ProTyr: 1.492 ± 0.423
0.0ProXaa: 0.0 ± 0.0
Gln
5.27GlnAla: 5.27 ± 1.11
0.597GlnCys: 0.597 ± 0.255
1.591GlnAsp: 1.591 ± 0.376
1.492GlnGlu: 1.492 ± 0.383
0.796GlnPhe: 0.796 ± 0.303
2.983GlnGly: 2.983 ± 0.559
1.193GlnHis: 1.193 ± 0.436
3.182GlnIle: 3.182 ± 0.448
1.691GlnLys: 1.691 ± 0.446
5.27GlnLeu: 5.27 ± 0.625
1.193GlnMet: 1.193 ± 0.358
0.696GlnAsn: 0.696 ± 0.222
3.481GlnPro: 3.481 ± 0.88
2.784GlnGln: 2.784 ± 0.786
5.469GlnArg: 5.469 ± 1.02
2.287GlnSer: 2.287 ± 0.414
0.994GlnThr: 0.994 ± 0.235
3.58GlnVal: 3.58 ± 0.445
0.398GlnTrp: 0.398 ± 0.223
0.696GlnTyr: 0.696 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
8.751ArgAla: 8.751 ± 1.031
1.193ArgCys: 1.193 ± 0.474
3.779ArgAsp: 3.779 ± 0.727
3.878ArgGlu: 3.878 ± 0.527
2.586ArgPhe: 2.586 ± 0.53
6.464ArgGly: 6.464 ± 0.689
2.884ArgHis: 2.884 ± 0.64
3.779ArgIle: 3.779 ± 0.579
2.784ArgLys: 2.784 ± 0.627
8.055ArgLeu: 8.055 ± 1.15
1.989ArgMet: 1.989 ± 0.433
1.193ArgAsn: 1.193 ± 0.408
4.276ArgPro: 4.276 ± 0.744
4.177ArgGln: 4.177 ± 0.689
8.254ArgArg: 8.254 ± 1.046
4.972ArgSer: 4.972 ± 0.73
4.475ArgThr: 4.475 ± 0.615
5.768ArgVal: 5.768 ± 0.639
1.094ArgTrp: 1.094 ± 0.301
1.79ArgTyr: 1.79 ± 0.564
0.0ArgXaa: 0.0 ± 0.0
Ser
8.453SerAla: 8.453 ± 1.2
0.597SerCys: 0.597 ± 0.201
2.586SerAsp: 2.586 ± 0.508
2.586SerGlu: 2.586 ± 0.453
1.79SerPhe: 1.79 ± 0.532
5.569SerGly: 5.569 ± 0.915
0.895SerHis: 0.895 ± 0.277
2.287SerIle: 2.287 ± 0.495
1.392SerLys: 1.392 ± 0.359
4.773SerLeu: 4.773 ± 0.85
1.193SerMet: 1.193 ± 0.314
2.088SerAsn: 2.088 ± 0.478
3.58SerPro: 3.58 ± 0.797
2.088SerGln: 2.088 ± 0.549
5.768SerArg: 5.768 ± 0.728
3.58SerSer: 3.58 ± 0.703
4.077SerThr: 4.077 ± 0.634
3.878SerVal: 3.878 ± 0.59
0.696SerTrp: 0.696 ± 0.235
2.188SerTyr: 2.188 ± 0.494
0.0SerXaa: 0.0 ± 0.0
Thr
8.95ThrAla: 8.95 ± 1.081
0.895ThrCys: 0.895 ± 0.256
4.475ThrAsp: 4.475 ± 0.698
2.784ThrGlu: 2.784 ± 0.466
2.188ThrPhe: 2.188 ± 0.492
6.265ThrGly: 6.265 ± 0.947
0.696ThrHis: 0.696 ± 0.243
3.182ThrIle: 3.182 ± 0.679
0.994ThrLys: 0.994 ± 0.308
6.762ThrLeu: 6.762 ± 0.862
1.193ThrMet: 1.193 ± 0.369
0.994ThrAsn: 0.994 ± 0.247
4.574ThrPro: 4.574 ± 0.891
2.088ThrGln: 2.088 ± 0.417
3.58ThrArg: 3.58 ± 0.642
3.58ThrSer: 3.58 ± 0.779
5.37ThrThr: 5.37 ± 0.862
3.679ThrVal: 3.679 ± 0.796
0.696ThrTrp: 0.696 ± 0.282
1.79ThrTyr: 1.79 ± 0.496
0.0ThrXaa: 0.0 ± 0.0
Val
9.049ValAla: 9.049 ± 1.158
0.497ValCys: 0.497 ± 0.273
3.779ValAsp: 3.779 ± 0.591
3.779ValGlu: 3.779 ± 0.661
1.392ValPhe: 1.392 ± 0.315
4.873ValGly: 4.873 ± 0.865
1.591ValHis: 1.591 ± 0.367
2.685ValIle: 2.685 ± 0.499
2.188ValLys: 2.188 ± 0.443
5.37ValLeu: 5.37 ± 0.729
1.79ValMet: 1.79 ± 0.373
1.989ValAsn: 1.989 ± 0.466
3.182ValPro: 3.182 ± 0.683
2.486ValGln: 2.486 ± 0.525
5.569ValArg: 5.569 ± 0.802
4.375ValSer: 4.375 ± 0.824
4.375ValThr: 4.375 ± 0.885
4.475ValVal: 4.475 ± 0.992
1.094ValTrp: 1.094 ± 0.345
0.895ValTyr: 0.895 ± 0.279
0.0ValXaa: 0.0 ± 0.0
Trp
1.392TrpAla: 1.392 ± 0.349
0.298TrpCys: 0.298 ± 0.13
0.597TrpAsp: 0.597 ± 0.212
0.497TrpGlu: 0.497 ± 0.201
0.398TrpPhe: 0.398 ± 0.228
0.994TrpGly: 0.994 ± 0.295
0.398TrpHis: 0.398 ± 0.219
0.597TrpIle: 0.597 ± 0.192
0.497TrpLys: 0.497 ± 0.183
1.691TrpLeu: 1.691 ± 0.399
0.298TrpMet: 0.298 ± 0.152
0.497TrpAsn: 0.497 ± 0.195
0.994TrpPro: 0.994 ± 0.27
0.696TrpGln: 0.696 ± 0.235
1.492TrpArg: 1.492 ± 0.393
0.597TrpSer: 0.597 ± 0.245
1.392TrpThr: 1.392 ± 0.343
0.597TrpVal: 0.597 ± 0.236
0.497TrpTrp: 0.497 ± 0.31
0.398TrpTyr: 0.398 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.691TyrAla: 1.691 ± 0.409
0.298TyrCys: 0.298 ± 0.166
1.392TyrAsp: 1.392 ± 0.369
0.994TyrGlu: 0.994 ± 0.319
1.094TyrPhe: 1.094 ± 0.426
2.188TyrGly: 2.188 ± 0.391
0.199TyrHis: 0.199 ± 0.127
0.696TyrIle: 0.696 ± 0.226
0.497TyrLys: 0.497 ± 0.202
2.188TyrLeu: 2.188 ± 0.454
0.696TyrMet: 0.696 ± 0.223
0.398TyrAsn: 0.398 ± 0.165
1.392TyrPro: 1.392 ± 0.364
0.796TyrGln: 0.796 ± 0.217
2.188TyrArg: 2.188 ± 0.467
1.293TyrSer: 1.293 ± 0.428
1.591TyrThr: 1.591 ± 0.349
1.989TyrVal: 1.989 ± 0.479
0.497TyrTrp: 0.497 ± 0.261
0.895TyrTyr: 0.895 ± 0.293
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (10057 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski