Sanxia tombus-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGG5|A0A1L3KGG5_9VIRU RNA-directed RNA polymerase OS=Sanxia tombus-like virus 8 OX=1923392 PE=4 SV=1
MM1 pKa = 7.16EE2 pKa = 6.71AIRR5 pKa = 11.84NIYY8 pKa = 9.56RR9 pKa = 11.84HH10 pKa = 5.38VAGAGPPPEE19 pKa = 4.36TDD21 pKa = 3.15DD22 pKa = 3.87TNLMEE27 pKa = 5.86SLEE30 pKa = 3.99QLLNANTTLNSTTIEE45 pKa = 4.18SYY47 pKa = 10.95KK48 pKa = 10.6KK49 pKa = 10.33VLGTVRR55 pKa = 11.84DD56 pKa = 3.78LTTICASLPTAATNTILDD74 pKa = 3.52EE75 pKa = 4.21RR76 pKa = 11.84LRR78 pKa = 11.84AEE80 pKa = 4.35TLTQQIAGIQHH91 pKa = 6.7GDD93 pKa = 3.37RR94 pKa = 11.84NEE96 pKa = 3.8QTAEE100 pKa = 4.09KK101 pKa = 10.42IEE103 pKa = 4.0LEE105 pKa = 3.92RR106 pKa = 11.84LKK108 pKa = 11.21AKK110 pKa = 10.47VKK112 pKa = 10.5ALEE115 pKa = 4.13EE116 pKa = 3.86QQILNDD122 pKa = 3.72RR123 pKa = 11.84ARR125 pKa = 11.84PPSIDD130 pKa = 3.35EE131 pKa = 4.21ALAFLKK137 pKa = 10.33IKK139 pKa = 10.7CLGKK143 pKa = 8.54TVDD146 pKa = 3.89EE147 pKa = 5.04TYY149 pKa = 9.8MAYY152 pKa = 10.26CGSILRR158 pKa = 11.84EE159 pKa = 3.94YY160 pKa = 10.61LLQRR164 pKa = 11.84AKK166 pKa = 10.8VSTRR170 pKa = 11.84LEE172 pKa = 3.72YY173 pKa = 10.88SIINGTMDD181 pKa = 2.93MHH183 pKa = 6.76MANIRR188 pKa = 11.84PQYY191 pKa = 10.94DD192 pKa = 3.58YY193 pKa = 11.37DD194 pKa = 3.44LHH196 pKa = 8.52NIMRR200 pKa = 11.84YY201 pKa = 8.23NDD203 pKa = 3.51CVNFVQRR210 pKa = 11.84STVRR214 pKa = 11.84VFNRR218 pKa = 11.84IYY220 pKa = 10.08TIPFTDD226 pKa = 3.22ICLKK230 pKa = 10.03SLNYY234 pKa = 9.75LRR236 pKa = 11.84PVGATEE242 pKa = 4.97NINHH246 pKa = 7.73PYY248 pKa = 9.94RR249 pKa = 11.84PRR251 pKa = 11.84FAYY254 pKa = 9.92LRR256 pKa = 11.84NILLIISILTAAYY269 pKa = 9.88NISPLFLGRR278 pKa = 11.84MKK280 pKa = 10.06HH281 pKa = 4.24TTIPNAPQPPLQPMPTGINLSTWMNIGQNILTTSSHH317 pKa = 5.76YY318 pKa = 10.67ASSSIDD324 pKa = 3.47SAHH327 pKa = 7.0GIMSSISTLWTAPASEE343 pKa = 4.63DD344 pKa = 4.23FISKK348 pKa = 10.37LSTRR352 pKa = 11.84FEE354 pKa = 3.99MDD356 pKa = 2.67EE357 pKa = 4.19KK358 pKa = 11.42SRR360 pKa = 11.84VASLLSLSLRR370 pKa = 11.84KK371 pKa = 8.98WDD373 pKa = 3.82QISTKK378 pKa = 10.65LQDD381 pKa = 3.83LSKK384 pKa = 10.73HH385 pKa = 6.3AIQHH389 pKa = 6.08SISLMDD395 pKa = 3.21ATLNHH400 pKa = 6.42SSVLTNTSYY409 pKa = 11.7NSAKK413 pKa = 10.95ALMTRR418 pKa = 11.84LEE420 pKa = 4.07PKK422 pKa = 9.04YY423 pKa = 9.51TSFLEE428 pKa = 4.25NTNTTLKK435 pKa = 9.44EE436 pKa = 4.25TIRR439 pKa = 11.84LLTHH443 pKa = 6.71TSPEE447 pKa = 4.12TCFEE451 pKa = 3.91YY452 pKa = 11.22AMNITEE458 pKa = 4.02AAINRR463 pKa = 11.84TTNFVDD469 pKa = 5.33FAAKK473 pKa = 10.47LSATRR478 pKa = 11.84LEE480 pKa = 4.12PVKK483 pKa = 11.07VKK485 pKa = 9.09ITAWSEE491 pKa = 3.88RR492 pKa = 11.84ACQEE496 pKa = 3.88TLIPAMEE503 pKa = 4.33TALSTTTYY511 pKa = 9.68SRR513 pKa = 11.84NYY515 pKa = 9.79LEE517 pKa = 4.58YY518 pKa = 10.45YY519 pKa = 9.4GLKK522 pKa = 9.19ATRR525 pKa = 11.84LLMEE529 pKa = 4.0TTLYY533 pKa = 10.88SLRR536 pKa = 11.84PVNWIVSNVNAYY548 pKa = 10.04YY549 pKa = 10.76EE550 pKa = 3.9NLIWKK555 pKa = 9.17RR556 pKa = 11.84RR557 pKa = 11.84WEE559 pKa = 3.98RR560 pKa = 11.84AQTISITLSFAAVALFITLMAIRR583 pKa = 11.84QWLSIQTVYY592 pKa = 10.59RR593 pKa = 11.84RR594 pKa = 11.84YY595 pKa = 7.87TVQPTSRR602 pKa = 11.84GRR604 pKa = 11.84TNNIPGIFRR613 pKa = 4.03

Molecular weight: 69.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGG5|A0A1L3KGG5_9VIRU RNA-directed RNA polymerase OS=Sanxia tombus-like virus 8 OX=1923392 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 4.69SPSKK6 pKa = 10.51RR7 pKa = 11.84RR8 pKa = 11.84FYY10 pKa = 10.58QQVVDD15 pKa = 4.11QIRR18 pKa = 11.84NGRR21 pKa = 11.84KK22 pKa = 6.86ITSGITPFTKK32 pKa = 10.33LEE34 pKa = 4.02KK35 pKa = 10.0MGSNKK40 pKa = 9.73YY41 pKa = 9.41KK42 pKa = 10.57APRR45 pKa = 11.84LIQARR50 pKa = 11.84HH51 pKa = 4.61PTFNIAYY58 pKa = 8.4GCYY61 pKa = 9.54IKK63 pKa = 10.6PLEE66 pKa = 4.37RR67 pKa = 11.84ANKK70 pKa = 9.94HH71 pKa = 5.29KK72 pKa = 10.89LQFGKK77 pKa = 9.01GTYY80 pKa = 10.2DD81 pKa = 3.35EE82 pKa = 5.01IGAKK86 pKa = 8.45IHH88 pKa = 6.0KK89 pKa = 10.11LSRR92 pKa = 11.84KK93 pKa = 6.58YY94 pKa = 10.49QYY96 pKa = 9.28YY97 pKa = 9.31TEE99 pKa = 5.16GDD101 pKa = 3.57HH102 pKa = 6.31KK103 pKa = 9.91TFDD106 pKa = 3.29AHH108 pKa = 5.03VTRR111 pKa = 11.84DD112 pKa = 3.41MLRR115 pKa = 11.84ICHH118 pKa = 5.18EE119 pKa = 4.63HH120 pKa = 6.4YY121 pKa = 10.32RR122 pKa = 11.84SCYY125 pKa = 8.71QQDD128 pKa = 3.28NQLRR132 pKa = 11.84RR133 pKa = 11.84LCSKK137 pKa = 7.65TLRR140 pKa = 11.84NKK142 pKa = 9.85ARR144 pKa = 11.84TRR146 pKa = 11.84EE147 pKa = 4.15GEE149 pKa = 4.07NYY151 pKa = 10.1SVVGTRR157 pKa = 11.84MSGDD161 pKa = 2.98VDD163 pKa = 3.38TGYY166 pKa = 11.59GNSIINYY173 pKa = 8.77YY174 pKa = 9.58ILKK177 pKa = 9.86KK178 pKa = 10.64LLGILRR184 pKa = 11.84IKK186 pKa = 10.69GDD188 pKa = 4.27AIVNGDD194 pKa = 4.0DD195 pKa = 4.65FILFTSSKK203 pKa = 10.47LDD205 pKa = 3.36SEE207 pKa = 4.22QCKK210 pKa = 10.39RR211 pKa = 11.84ILRR214 pKa = 11.84EE215 pKa = 3.97FNMEE219 pKa = 4.03TEE221 pKa = 4.46MGEE224 pKa = 4.19STNNIHH230 pKa = 7.15HH231 pKa = 7.03IEE233 pKa = 4.14FCRR236 pKa = 11.84CRR238 pKa = 11.84LIYY241 pKa = 10.61HH242 pKa = 7.23PDD244 pKa = 3.09GHH246 pKa = 6.52PTMAFDD252 pKa = 4.94PDD254 pKa = 4.02RR255 pKa = 11.84LQKK258 pKa = 10.39IYY260 pKa = 10.73GATYY264 pKa = 10.24LPWTDD269 pKa = 3.44EE270 pKa = 3.94QYY272 pKa = 10.79TGYY275 pKa = 10.48LQVIHH280 pKa = 6.75HH281 pKa = 7.19ANFMINQNSPAHH293 pKa = 5.49RR294 pKa = 11.84QWRR297 pKa = 11.84PLLSLEE303 pKa = 4.83DD304 pKa = 3.72KK305 pKa = 10.31LKK307 pKa = 11.01KK308 pKa = 10.83YY309 pKa = 7.2MTHH312 pKa = 5.42SLEE315 pKa = 4.31RR316 pKa = 11.84VFAKK320 pKa = 10.36QSNNRR325 pKa = 11.84VYY327 pKa = 10.41KK328 pKa = 9.68WDD330 pKa = 3.91YY331 pKa = 8.44LTPSYY336 pKa = 10.87IEE338 pKa = 4.96AYY340 pKa = 9.59PNYY343 pKa = 10.28KK344 pKa = 8.72IDD346 pKa = 3.57KK347 pKa = 8.46PQYY350 pKa = 8.79PYY352 pKa = 10.52YY353 pKa = 10.77KK354 pKa = 9.87PVAPQKK360 pKa = 10.53RR361 pKa = 11.84IVINHH366 pKa = 6.48NIKK369 pKa = 10.48EE370 pKa = 4.13LFIVV374 pKa = 3.93

Molecular weight: 44.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

987

374

613

493.5

56.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.484 ± 1.498

1.418 ± 0.274

4.053 ± 0.784

5.37 ± 0.337

2.837 ± 0.387

3.647 ± 1.03

2.837 ± 0.873

8.004 ± 0.151

5.775 ± 1.684

9.524 ± 1.396

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.199

5.978 ± 0.22

4.357 ± 0.114

3.951 ± 0.522

7.092 ± 0.239

6.89 ± 1.096

8.815 ± 1.938

3.546 ± 0.042

1.114 ± 0.189

5.572 ± 1.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski