Cordyline virus 1
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7CT66|E7CT66_9CLOS p6 OS=Cordyline virus 1 OX=937809 PE=4 SV=1
MM1 pKa = 7.55 PTFCRR6 pKa = 11.84 AGVDD10 pKa = 4.04 FGTTFSTISAFVDD23 pKa = 4.48 EE24 pKa = 4.81 VATPLFLEE32 pKa = 5.23 GSPFIPTVITFFDD45 pKa = 3.56 NKK47 pKa = 10.05 VVIGEE52 pKa = 4.08 LAKK55 pKa = 10.27 TISEE59 pKa = 4.35 VVKK62 pKa = 11.17 DD63 pKa = 3.56 NVTYY67 pKa = 10.71 FDD69 pKa = 3.73 LKK71 pKa = 10.3 RR72 pKa = 11.84 WVGVNEE78 pKa = 4.0 KK79 pKa = 10.9 NFIKK83 pKa = 10.48 LKK85 pKa = 10.58 EE86 pKa = 4.04 KK87 pKa = 10.42 LQPSYY92 pKa = 10.83 EE93 pKa = 4.25 CKK95 pKa = 10.43 LEE97 pKa = 4.36 KK98 pKa = 10.75 GEE100 pKa = 4.72 CYY102 pKa = 9.54 MGGIGVRR109 pKa = 11.84 TVFRR113 pKa = 11.84 SVGFLIATYY122 pKa = 10.9 LDD124 pKa = 3.78 VLTKK128 pKa = 10.71 LFEE131 pKa = 4.16 EE132 pKa = 5.09 KK133 pKa = 10.77 YY134 pKa = 10.32 DD135 pKa = 4.01 VKK137 pKa = 10.19 ITNLNVSVPADD148 pKa = 4.42 FYY150 pKa = 10.3 TFQRR154 pKa = 11.84 SYY156 pKa = 10.32 MRR158 pKa = 11.84 NAVNNLGIKK167 pKa = 8.8 VDD169 pKa = 3.6 RR170 pKa = 11.84 MINEE174 pKa = 4.14 PSAAALHH181 pKa = 6.58 SILSNPEE188 pKa = 3.27 FTDD191 pKa = 4.15 FVIFDD196 pKa = 4.08 FGGGTFDD203 pKa = 3.81 VSYY206 pKa = 10.39 IKK208 pKa = 10.71 KK209 pKa = 9.07 KK210 pKa = 10.75 GKK212 pKa = 10.1 IIMICDD218 pKa = 3.59 TQGDD222 pKa = 4.46 LFLGGRR228 pKa = 11.84 DD229 pKa = 3.02 IDD231 pKa = 4.91 KK232 pKa = 10.93 SIQHH236 pKa = 5.75 YY237 pKa = 10.07 FFGKK241 pKa = 10.05 YY242 pKa = 9.99 SIEE245 pKa = 3.6 IHH247 pKa = 6.33 PFSLSYY253 pKa = 10.0 MKK255 pKa = 10.68 EE256 pKa = 3.91 GVSTGKK262 pKa = 9.69 SQSFNVLSSNKK273 pKa = 9.66 EE274 pKa = 3.69 INHH277 pKa = 5.44 VNFSKK282 pKa = 11.22 DD283 pKa = 3.37 EE284 pKa = 4.32 LNSIVSPFAKK294 pKa = 9.96 RR295 pKa = 11.84 SCEE298 pKa = 3.62 ILKK301 pKa = 10.74 AVIDD305 pKa = 4.04 RR306 pKa = 11.84 NEE308 pKa = 3.6 ITNAVICMVGGSSLLTEE325 pKa = 5.07 VYY327 pKa = 10.64 NQVNNVAKK335 pKa = 9.24 GTNNKK340 pKa = 8.95 IFRR343 pKa = 11.84 DD344 pKa = 3.55 EE345 pKa = 3.94 NLRR348 pKa = 11.84 LSVSFGCSCLHH359 pKa = 6.92 FFTDD363 pKa = 5.52 DD364 pKa = 3.62 PDD366 pKa = 3.48 FTYY369 pKa = 10.61 IDD371 pKa = 4.09 VNSHH375 pKa = 5.79 CVFEE379 pKa = 5.0 IDD381 pKa = 4.7 EE382 pKa = 4.24 MFKK385 pKa = 10.75 PSVLIRR391 pKa = 11.84 KK392 pKa = 7.67 PMPIPYY398 pKa = 8.97 TLRR401 pKa = 11.84 QEE403 pKa = 4.21 KK404 pKa = 10.27 QNNNKK409 pKa = 9.5 FLTAVDD415 pKa = 3.67 IYY417 pKa = 10.91 EE418 pKa = 5.14 GDD420 pKa = 3.93 SQWFLDD426 pKa = 4.12 CQVLIKK432 pKa = 10.56 DD433 pKa = 4.18 VYY435 pKa = 9.84 STDD438 pKa = 3.49 AVSSLGSGYY447 pKa = 10.61 YY448 pKa = 9.36 RR449 pKa = 11.84 VIQYY453 pKa = 11.09 DD454 pKa = 3.49 LDD456 pKa = 3.94 GNINVWIEE464 pKa = 4.11 NKK466 pKa = 10.54 DD467 pKa = 3.57 STNKK471 pKa = 10.05 RR472 pKa = 11.84 NLKK475 pKa = 10.45 SLITSHH481 pKa = 6.18 KK482 pKa = 8.79 TIKK485 pKa = 10.62 LEE487 pKa = 3.76 NFEE490 pKa = 4.39 RR491 pKa = 11.84 VQTGSSSLYY500 pKa = 8.67 CTVAEE505 pKa = 4.45 LIKK508 pKa = 10.43 YY509 pKa = 9.3 HH510 pKa = 6.96 KK511 pKa = 10.09 LDD513 pKa = 3.92 KK514 pKa = 10.96 NIEE517 pKa = 4.53 AIDD520 pKa = 3.66 SNLFFKK526 pKa = 10.71 IKK528 pKa = 10.37 DD529 pKa = 3.66 YY530 pKa = 11.5 IEE532 pKa = 4.4 INGGISKK539 pKa = 10.67 YY540 pKa = 10.39 IEE542 pKa = 3.96 TLRR545 pKa = 11.84 KK546 pKa = 10.14 NGVQII551 pKa = 4.32
Molecular weight: 62.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.758
IPC2_protein 5.855
IPC_protein 5.881
Toseland 6.097
ProMoST 6.211
Dawson 6.135
Bjellqvist 6.148
Wikipedia 6.122
Rodwell 6.109
Grimsley 6.186
Solomon 6.135
Lehninger 6.122
Nozaki 6.376
DTASelect 6.561
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.478
Patrickios 4.101
IPC_peptide 6.148
IPC2_peptide 6.389
IPC2.peptide.svr19 6.49
Protein with the highest isoelectric point:
>tr|E7CT65|E7CT65_9CLOS HSP70h OS=Cordyline virus 1 OX=937809 PE=3 SV=1
MM1 pKa = 7.58 IFLFFLFFLLFLFLLNLCTSPSNKK25 pKa = 9.6 GFIVHH30 pKa = 5.68 KK31 pKa = 10.08 QIYY34 pKa = 8.52 YY35 pKa = 10.52 SPP37 pKa = 3.86
Molecular weight: 4.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.761
IPC2_protein 8.39
IPC_protein 8.785
Toseland 8.916
ProMoST 8.887
Dawson 9.092
Bjellqvist 9.107
Wikipedia 9.18
Rodwell 9.121
Grimsley 8.77
Solomon 9.516
Lehninger 9.502
Nozaki 9.194
DTASelect 8.975
Thurlkill 9.019
EMBOSS 9.282
Sillero 9.253
Patrickios 2.409
IPC_peptide 9.502
IPC2_peptide 8.244
IPC2.peptide.svr19 7.88
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
5415
37
2360
541.5
62.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.358 ± 0.413
1.773 ± 0.229
5.854 ± 0.281
5.669 ± 0.558
6.464 ± 0.431
3.767 ± 0.359
1.699 ± 0.179
7.701 ± 0.591
8.79 ± 0.398
9.03 ± 0.331
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.161 ± 0.274
7.313 ± 0.298
2.77 ± 0.363
2.77 ± 0.285
4.543 ± 0.285
7.553 ± 0.374
5.706 ± 0.586
7.018 ± 0.538
0.517 ± 0.109
4.543 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here