Streptococcus phage CHPC1073

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FAU5|A0A3G8FAU5_9CAUD Single-stranded DNA-binding protein OS=Streptococcus phage CHPC1073 OX=2365023 GN=CHPC1073_0035 PE=4 SV=1
MM1 pKa = 7.13VNWVDD6 pKa = 4.58ANGNDD11 pKa = 3.45IPDD14 pKa = 4.08GKK16 pKa = 10.99SEE18 pKa = 4.08DD19 pKa = 4.06FKK21 pKa = 11.35PGMFFSFAGDD31 pKa = 3.45EE32 pKa = 4.32VNITDD37 pKa = 3.77TGEE40 pKa = 3.65GGYY43 pKa = 10.61YY44 pKa = 9.76GGYY47 pKa = 8.0YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FAL9|A0A3G8FAL9_9CAUD Transcriptional regulator OS=Streptococcus phage CHPC1073 OX=2365023 GN=CHPC1073_0030 PE=4 SV=1
MM1 pKa = 6.65TTLSKK6 pKa = 10.54RR7 pKa = 11.84DD8 pKa = 3.43NQLRR12 pKa = 11.84ILTSLLLISIVINVTTIIRR31 pKa = 11.84VVNRR35 pKa = 11.84PIEE38 pKa = 4.03TVVIHH43 pKa = 6.42KK44 pKa = 10.22VDD46 pKa = 3.47NATVLHH52 pKa = 6.1GKK54 pKa = 8.05ITGKK58 pKa = 10.27QMIGKK63 pKa = 9.61LYY65 pKa = 10.34TIDD68 pKa = 3.41CGAYY72 pKa = 10.1GKK74 pKa = 10.6FLVTKK79 pKa = 8.57EE80 pKa = 4.3QYY82 pKa = 11.14DD83 pKa = 4.0SVQVGDD89 pKa = 5.03DD90 pKa = 3.5VPSYY94 pKa = 11.25LKK96 pKa = 10.97GRR98 pKa = 11.84GQQ100 pKa = 3.22

Molecular weight: 11.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

10640

40

1593

236.4

26.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.438 ± 0.527

0.62 ± 0.149

6.551 ± 0.224

6.617 ± 0.439

4.305 ± 0.244

6.579 ± 0.495

1.466 ± 0.1

6.72 ± 0.269

8.412 ± 0.558

8.12 ± 0.357

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.164

6.476 ± 0.287

3.017 ± 0.218

4.126 ± 0.204

4.182 ± 0.264

6.222 ± 0.228

6.372 ± 0.314

6.118 ± 0.298

1.429 ± 0.151

3.882 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski