Streptococcus phage Javan496

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B8A5|A0A4D6B8A5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan496 OX=2548210 GN=Javan496_0044 PE=4 SV=1
MM1 pKa = 7.11FVDD4 pKa = 4.52MYY6 pKa = 11.16AGQFTVDD13 pKa = 4.27DD14 pKa = 4.81LKK16 pKa = 11.47LSDD19 pKa = 3.9MSVLDD24 pKa = 4.18TLNDD28 pKa = 3.9LYY30 pKa = 10.68IAALGGEE37 pKa = 4.33EE38 pKa = 4.65EE39 pKa = 4.35EE40 pKa = 5.46GEE42 pKa = 4.39TEE44 pKa = 4.94KK45 pKa = 11.14KK46 pKa = 9.68EE47 pKa = 3.95QQ48 pKa = 3.38

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B8B8|A0A4D6B8B8_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan496 OX=2548210 GN=Javan496_0039 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.22KK7 pKa = 9.09KK8 pKa = 10.4RR9 pKa = 11.84KK10 pKa = 8.96LEE12 pKa = 3.96TAIVLLIKK20 pKa = 10.27EE21 pKa = 4.31VAEE24 pKa = 3.97LRR26 pKa = 11.84SIVSANAKK34 pKa = 8.06ATNNEE39 pKa = 3.65LAAVKK44 pKa = 10.33SAILDD49 pKa = 3.45NQVAIKK55 pKa = 10.63SIGDD59 pKa = 3.5EE60 pKa = 4.1VGHH63 pKa = 6.84IKK65 pKa = 10.4QNYY68 pKa = 6.67KK69 pKa = 10.51RR70 pKa = 11.84KK71 pKa = 8.22WRR73 pKa = 11.84KK74 pKa = 8.88

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

9476

41

725

193.4

21.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.271 ± 0.755

0.644 ± 0.178

6.543 ± 0.35

7.197 ± 0.378

3.789 ± 0.28

6.638 ± 0.419

1.266 ± 0.148

6.923 ± 0.318

9.107 ± 0.397

8.833 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.188

5.604 ± 0.251

2.702 ± 0.248

3.704 ± 0.267

4.095 ± 0.224

6.216 ± 0.404

6.258 ± 0.252

6.205 ± 0.29

1.087 ± 0.116

3.525 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski