Rhodobacter phage RcapMu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8GWF6|G8GWF6_9CAUD Tape measure protein OS=Rhodobacter phage RcapMu OX=1105286 GN=RcapMu51 PE=4 SV=1
MM1 pKa = 7.39ATPDD5 pKa = 3.07IAAQFAAMGAEE16 pKa = 3.68IEE18 pKa = 4.35APVTEE23 pKa = 4.65DD24 pKa = 4.8VIEE27 pKa = 4.01IWQCHH32 pKa = 5.3ARR34 pKa = 11.84TVAAFFDD41 pKa = 4.04IQTQWRR47 pKa = 11.84VTEE50 pKa = 3.83VHH52 pKa = 5.71GALWLGLDD60 pKa = 3.64YY61 pKa = 11.53SGVDD65 pKa = 3.15VALRR69 pKa = 11.84RR70 pKa = 11.84GGYY73 pKa = 10.43DD74 pKa = 3.3DD75 pKa = 5.77VDD77 pKa = 4.15FADD80 pKa = 3.98LQLMEE85 pKa = 4.93RR86 pKa = 11.84AAIDD90 pKa = 3.51AFVGRR95 pKa = 4.68

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8GWC3|G8GWC3_9CAUD LacI-like regulatory protein OS=Rhodobacter phage RcapMu OX=1105286 GN=RcapMu18 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.37VLQHH6 pKa = 6.73LALPLTAPTIQIALALEE23 pKa = 4.27VSPKK27 pKa = 10.11RR28 pKa = 11.84VSKK31 pKa = 10.72AAASLVDD38 pKa = 3.41RR39 pKa = 11.84GYY41 pKa = 10.63ISRR44 pKa = 11.84DD45 pKa = 2.79RR46 pKa = 11.84PGIYY50 pKa = 10.12KK51 pKa = 8.46ITFRR55 pKa = 11.84GLQAIADD62 pKa = 4.12GKK64 pKa = 10.85VITSGPVDD72 pKa = 3.24ASSNVLSRR80 pKa = 11.84PEE82 pKa = 3.96TNGFRR87 pKa = 11.84DD88 pKa = 3.76RR89 pKa = 11.84VWRR92 pKa = 11.84SMRR95 pKa = 11.84LRR97 pKa = 11.84GTFTISDD104 pKa = 4.16VVCDD108 pKa = 3.84AVDD111 pKa = 3.74GEE113 pKa = 4.4RR114 pKa = 11.84DD115 pKa = 3.22AMTNAGSYY123 pKa = 10.27IRR125 pKa = 11.84VLKK128 pKa = 10.67SSGYY132 pKa = 9.34VSEE135 pKa = 4.35LPGRR139 pKa = 11.84RR140 pKa = 11.84KK141 pKa = 8.57GTKK144 pKa = 9.31PGSNGLKK151 pKa = 10.28RR152 pKa = 11.84FRR154 pKa = 11.84LDD156 pKa = 3.19RR157 pKa = 11.84NTGPRR162 pKa = 11.84APLHH166 pKa = 5.91RR167 pKa = 11.84SKK169 pKa = 11.12LGIVHH174 pKa = 7.33DD175 pKa = 4.54FNTGEE180 pKa = 5.37DD181 pKa = 3.66IPCRR185 pKa = 3.77

Molecular weight: 20.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12543

35

1142

216.3

23.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.466 ± 0.432

0.678 ± 0.109

6.019 ± 0.193

5.979 ± 0.337

3.133 ± 0.212

8.515 ± 0.33

1.706 ± 0.176

5.094 ± 0.284

4.106 ± 0.337

8.387 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.214

2.28 ± 0.165

5.055 ± 0.184

3.532 ± 0.251

7.024 ± 0.356

5.342 ± 0.261

6.131 ± 0.37

7.04 ± 0.308

1.905 ± 0.203

2.137 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski