Enterococcus phage EFC-1
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097BY35|A0A097BY35_9CAUD Methyltransferase OS=Enterococcus phage EFC-1 OX=1486428 PE=4 SV=1
MM1 pKa = 7.66 YY2 pKa = 10.78 YY3 pKa = 9.81 PDD5 pKa = 3.57 EE6 pKa = 4.95 DD7 pKa = 5.3 KK8 pKa = 11.39 NVEE11 pKa = 3.81 WTIDD15 pKa = 3.62 DD16 pKa = 3.67 DD17 pKa = 4.35 TGFIAPLINLEE28 pKa = 3.85 NGMWGMIDD36 pKa = 4.61 KK37 pKa = 10.34 YY38 pKa = 11.64 VLMQSTGFKK47 pKa = 10.25 DD48 pKa = 3.65 DD49 pKa = 3.71 NGVEE53 pKa = 4.03 IFEE56 pKa = 4.33 GDD58 pKa = 3.6 VVNIHH63 pKa = 6.4 WFYY66 pKa = 11.04 IDD68 pKa = 4.39 YY69 pKa = 10.99 DD70 pKa = 4.21 PEE72 pKa = 4.04 TLGAIEE78 pKa = 4.59 NEE80 pKa = 3.75 ATAEE84 pKa = 4.05 KK85 pKa = 10.83 VVITKK90 pKa = 9.97 EE91 pKa = 3.88 FGNLGFWWNSGDD103 pKa = 4.03 DD104 pKa = 3.88 WVDD107 pKa = 3.33 LATLALTVQLHH118 pKa = 5.43 EE119 pKa = 4.72 EE120 pKa = 4.11 SFEE123 pKa = 4.09 LLGNVHH129 pKa = 6.74 EE130 pKa = 4.6 NPEE133 pKa = 4.24 LLEE136 pKa = 4.38 KK137 pKa = 10.76 ASEE140 pKa = 4.14 EE141 pKa = 4.21 DD142 pKa = 3.57 DD143 pKa = 4.65 SEE145 pKa = 4.44 VV146 pKa = 3.26
Molecular weight: 16.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.051
IPC2_protein 3.694
IPC_protein 3.668
Toseland 3.478
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.503
Grimsley 3.389
Solomon 3.643
Lehninger 3.592
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.516
EMBOSS 3.579
Sillero 3.795
Patrickios 2.969
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.746
Protein with the highest isoelectric point:
>tr|A0A097BYA1|A0A097BYA1_9CAUD Tapemeasure domain-containing protein OS=Enterococcus phage EFC-1 OX=1486428 PE=4 SV=1
MM1 pKa = 7.63 ARR3 pKa = 11.84 NQVRR7 pKa = 11.84 INGLAGISKK16 pKa = 10.06 KK17 pKa = 10.56 LKK19 pKa = 10.57 RR20 pKa = 11.84 NAQLDD25 pKa = 3.93 DD26 pKa = 3.58 VKK28 pKa = 11.14 KK29 pKa = 10.31 VVRR32 pKa = 11.84 NNTAEE37 pKa = 4.08 LTANMQAEE45 pKa = 4.34 AGKK48 pKa = 10.5 VLTGHH53 pKa = 6.39 WEE55 pKa = 3.96 GKK57 pKa = 10.35 KK58 pKa = 9.82 FVKK61 pKa = 8.91 PTGATKK67 pKa = 10.42 RR68 pKa = 11.84 SIVMRR73 pKa = 11.84 LSNNGFSGHH82 pKa = 6.26 TGPGTEE88 pKa = 3.92 YY89 pKa = 10.97 APYY92 pKa = 9.6 LIHH95 pKa = 6.37 GTRR98 pKa = 11.84 FMVKK102 pKa = 9.1 RR103 pKa = 11.84 DD104 pKa = 3.69 FFLPPLKK111 pKa = 9.69 QQKK114 pKa = 9.72 VKK116 pKa = 10.51 FRR118 pKa = 11.84 TDD120 pKa = 3.28 LEE122 pKa = 4.32 RR123 pKa = 11.84 LMKK126 pKa = 10.6
Molecular weight: 14.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 9.853
IPC_protein 10.35
Toseland 11.067
ProMoST 10.687
Dawson 11.111
Bjellqvist 10.76
Wikipedia 11.272
Rodwell 11.535
Grimsley 11.125
Solomon 11.228
Lehninger 11.213
Nozaki 11.023
DTASelect 10.76
Thurlkill 11.038
EMBOSS 11.447
Sillero 11.038
Patrickios 11.272
IPC_peptide 11.242
IPC2_peptide 9.253
IPC2.peptide.svr19 8.7
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
59
0
59
12628
25
1135
214.0
24.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.414 ± 0.424
0.665 ± 0.125
6.296 ± 0.228
8.077 ± 0.348
3.484 ± 0.203
5.836 ± 0.267
1.37 ± 0.2
6.889 ± 0.247
9.075 ± 0.4
8.315 ± 0.299
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.732 ± 0.162
6.058 ± 0.251
3.08 ± 0.197
4.458 ± 0.319
3.69 ± 0.241
6.723 ± 0.318
5.86 ± 0.335
6.066 ± 0.202
1.212 ± 0.129
3.698 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here