Bacillus shackletonii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Margalitia

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q3TMU8|A0A0Q3TMU8_9BACI Uncharacterized protein OS=Bacillus shackletonii OX=157838 GN=AN964_17210 PE=4 SV=1
MM1 pKa = 8.09AIRR4 pKa = 11.84NFIVNGDD11 pKa = 4.0FEE13 pKa = 6.7DD14 pKa = 5.13GSLDD18 pKa = 3.18NWLEE22 pKa = 3.8EE23 pKa = 4.01NAAITTEE30 pKa = 4.72FSHH33 pKa = 6.78SGYY36 pKa = 10.78YY37 pKa = 9.09SARR40 pKa = 11.84LAGGDD45 pKa = 3.22INSYY49 pKa = 10.24LVQFVDD55 pKa = 3.96ANPGEE60 pKa = 4.43SFEE63 pKa = 4.63FLASLAKK70 pKa = 10.5VGSDD74 pKa = 3.31PSPPVALLVVFYY86 pKa = 11.08DD87 pKa = 3.61NALDD91 pKa = 3.72YY92 pKa = 11.18LGYY95 pKa = 10.83SLIDD99 pKa = 4.2NIPPGALPSADD110 pKa = 3.2NDD112 pKa = 3.13NWLEE116 pKa = 4.12IYY118 pKa = 10.62HH119 pKa = 5.79EE120 pKa = 4.36TSPAPLGTTQALVVINKK137 pKa = 9.64LPFPGSADD145 pKa = 3.12ILVDD149 pKa = 5.56DD150 pKa = 4.81IQLLDD155 pKa = 3.63TTNTGLPGITGPTGPPGATGPAGPTGPAGPPGATGPAGPTGPAGPPGTPGATGPAGATGPAGATGPAGATGPAGATGPAGVTGPAGATGPAGATGPAGVTGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGVTGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGVTGPAGVTGPAGATGPAGATGPAGATGPAGVTGPAGATGPAGATGPAGVTGPAGVTGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGVTGPAGATGPAGATGPAGATGPAGVTGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGATGPAGVTGPAGVTGPAGATGPAGATGPAGATGPAGATGPAGVTGPAGPTGATGTGLAAGFSARR605 pKa = 11.84NQGGITVGVGGAVPFNTPEE624 pKa = 3.97TSFGTDD630 pKa = 2.24ITQPTTTTFSLNAPGFYY647 pKa = 9.83SVRR650 pKa = 11.84FILYY654 pKa = 5.94TTAVSLLGGAEE665 pKa = 3.77ITVNGTPVSGDD676 pKa = 3.24FALAAVGAPLIGEE689 pKa = 4.42TIVNVTTAPVTVSIVVTGVALSLSTGTNAHH719 pKa = 5.8IQIEE723 pKa = 4.3KK724 pKa = 10.34LAA726 pKa = 4.17

Molecular weight: 63.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q3TA23|A0A0Q3TA23_9BACI Uncharacterized protein OS=Bacillus shackletonii OX=157838 GN=AN964_22120 PE=4 SV=1
MM1 pKa = 7.55TEE3 pKa = 3.94FPEE6 pKa = 4.78GNNEE10 pKa = 3.96MFAGPTSRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84SSSRR26 pKa = 11.84RR27 pKa = 11.84SSRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84SSSSSSSSRR41 pKa = 11.84RR42 pKa = 11.84SSRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84SSSRR51 pKa = 11.84RR52 pKa = 11.84SSSRR56 pKa = 11.84RR57 pKa = 11.84SSSRR61 pKa = 11.84RR62 pKa = 11.84SSRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84SSSRR71 pKa = 11.84RR72 pKa = 11.84SSRR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84SSSRR81 pKa = 11.84RR82 pKa = 11.84SSRR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84SSRR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 11.84SSRR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84SSSRR101 pKa = 11.84RR102 pKa = 11.84SSRR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84TSGFLFF113 pKa = 4.23

Molecular weight: 13.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4690

0

4690

1351557

26

1518

288.2

32.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.488 ± 0.036

0.775 ± 0.011

4.931 ± 0.027

7.06 ± 0.043

4.72 ± 0.031

6.761 ± 0.031

2.149 ± 0.016

8.516 ± 0.04

7.464 ± 0.035

9.732 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.017

4.786 ± 0.028

3.607 ± 0.02

3.626 ± 0.023

3.839 ± 0.022

6.115 ± 0.025

5.234 ± 0.023

6.721 ± 0.03

1.065 ± 0.014

3.642 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski