Eubenangee virus
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9ZXS0|H9ZXS0_9REOV VP6 OS=Eubenangee virus OX=40056 PE=4 SV=1
MM1 pKa = 7.4 EE2 pKa = 5.4 SKK4 pKa = 9.47 TVRR7 pKa = 11.84 RR8 pKa = 11.84 VFTKK12 pKa = 9.0 QVCILDD18 pKa = 4.05 PGCTTICGKK27 pKa = 9.57 IARR30 pKa = 11.84 EE31 pKa = 4.08 AKK33 pKa = 10.3 CPYY36 pKa = 9.95 AQLKK40 pKa = 7.91 TGRR43 pKa = 11.84 VCGFQAVKK51 pKa = 10.55 SPPPKK56 pKa = 10.45 AYY58 pKa = 9.16 ILEE61 pKa = 4.1 ITRR64 pKa = 11.84 PGAYY68 pKa = 9.3 RR69 pKa = 11.84 IMDD72 pKa = 4.04 GQDD75 pKa = 3.52 QISMMIEE82 pKa = 3.56 EE83 pKa = 4.9 GGIEE87 pKa = 3.88 ITTDD91 pKa = 2.77 RR92 pKa = 11.84 WEE94 pKa = 3.76 EE95 pKa = 3.5 WKK97 pKa = 11.19 YY98 pKa = 10.99 EE99 pKa = 4.19 VVTAMPMTALVDD111 pKa = 3.87 TPKK114 pKa = 11.07 GKK116 pKa = 10.32 VDD118 pKa = 4.66 AEE120 pKa = 3.98 LKK122 pKa = 7.97 YY123 pKa = 11.11 AKK125 pKa = 10.37 GAGLVPAYY133 pKa = 8.44 TKK135 pKa = 11.16 NEE137 pKa = 3.72 FDD139 pKa = 3.82 RR140 pKa = 11.84 RR141 pKa = 11.84 EE142 pKa = 4.16 LPSLPGVGISEE153 pKa = 3.84 ISVRR157 pKa = 11.84 DD158 pKa = 3.41 LRR160 pKa = 11.84 AKK162 pKa = 10.53 LKK164 pKa = 10.82 AEE166 pKa = 3.39 RR167 pKa = 11.84 VMDD170 pKa = 3.92 NPRR173 pKa = 11.84 SEE175 pKa = 4.74 HH176 pKa = 6.34 RR177 pKa = 11.84 DD178 pKa = 3.23 DD179 pKa = 3.81 RR180 pKa = 11.84 RR181 pKa = 11.84 KK182 pKa = 8.9 STRR185 pKa = 11.84 DD186 pKa = 3.14 EE187 pKa = 4.3 TPVFDD192 pKa = 4.26 VSEE195 pKa = 4.02 MLKK198 pKa = 10.29 KK199 pKa = 10.09 VEE201 pKa = 4.04 EE202 pKa = 4.13 MRR204 pKa = 11.84 SEE206 pKa = 3.93 EE207 pKa = 4.09 RR208 pKa = 11.84 SWSDD212 pKa = 3.31 EE213 pKa = 4.12 MEE215 pKa = 4.79 DD216 pKa = 3.54 EE217 pKa = 4.35 AAGGSVDD224 pKa = 3.62 VQQDD228 pKa = 3.7 DD229 pKa = 3.9 GALWEE234 pKa = 5.16 DD235 pKa = 3.84 EE236 pKa = 4.33 TAKK239 pKa = 11.08 DD240 pKa = 3.38 VDD242 pKa = 4.3 DD243 pKa = 4.44 EE244 pKa = 5.12 SKK246 pKa = 11.63 VMLEE250 pKa = 4.89 DD251 pKa = 2.99 ITEE254 pKa = 4.08 EE255 pKa = 4.12 EE256 pKa = 4.54 EE257 pKa = 4.26 PFNATNFITEE267 pKa = 4.02 AFVTNVSAYY276 pKa = 8.23 MKK278 pKa = 10.57 SGDD281 pKa = 3.53 KK282 pKa = 10.42 RR283 pKa = 11.84 VVGLASSFPKK293 pKa = 10.45 SAGSFDD299 pKa = 4.33 SVVCVKK305 pKa = 10.19 KK306 pKa = 10.81 ANWMNVPLFDD316 pKa = 4.25 VDD318 pKa = 3.85 VGKK321 pKa = 10.62 KK322 pKa = 10.5 GYY324 pKa = 8.14 GLRR327 pKa = 11.84 MIGDD331 pKa = 3.73 SAKK334 pKa = 10.7 LYY336 pKa = 9.41 IVKK339 pKa = 10.32 RR340 pKa = 11.84 GLSVMVLPAGVV351 pKa = 3.07
Molecular weight: 39.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.045
IPC2_protein 5.054
IPC_protein 4.978
Toseland 4.825
ProMoST 5.067
Dawson 4.902
Bjellqvist 5.054
Wikipedia 4.762
Rodwell 4.813
Grimsley 4.736
Solomon 4.902
Lehninger 4.863
Nozaki 5.016
DTASelect 5.156
Thurlkill 4.813
EMBOSS 4.774
Sillero 5.08
Patrickios 4.368
IPC_peptide 4.914
IPC2_peptide 5.067
IPC2.peptide.svr19 5.137
Protein with the highest isoelectric point:
>tr|H9ZXR3|H9ZXR3_9REOV Outer capsid protein VP2 OS=Eubenangee virus OX=40056 PE=3 SV=1
MM1 pKa = 6.93 VATVRR6 pKa = 11.84 GVEE9 pKa = 3.87 IVDD12 pKa = 3.5 RR13 pKa = 11.84 VLKK16 pKa = 10.51 RR17 pKa = 11.84 FYY19 pKa = 9.35 PTAEE23 pKa = 3.65 FDD25 pKa = 3.55 YY26 pKa = 11.3 TRR28 pKa = 11.84 GFWHH32 pKa = 8.01 LYY34 pKa = 9.68 RR35 pKa = 11.84 FSTNIRR41 pKa = 11.84 AIRR44 pKa = 11.84 RR45 pKa = 11.84 KK46 pKa = 10.22 KK47 pKa = 7.44 GTKK50 pKa = 7.07 YY51 pKa = 10.49 QRR53 pKa = 11.84 DD54 pKa = 3.98 DD55 pKa = 3.51 EE56 pKa = 4.57 VLANLVDD63 pKa = 3.89 RR64 pKa = 11.84 RR65 pKa = 11.84 TEE67 pKa = 3.7 TLYY70 pKa = 11.07 GIRR73 pKa = 11.84 VLNEE77 pKa = 3.63 ASWNEE82 pKa = 3.62 LTEE85 pKa = 4.44 EE86 pKa = 4.09 YY87 pKa = 10.64 NKK89 pKa = 10.31 RR90 pKa = 11.84 SRR92 pKa = 11.84 GLKK95 pKa = 9.28 IFEE98 pKa = 4.21 NSVLDD103 pKa = 4.1 HH104 pKa = 7.05 EE105 pKa = 4.72 EE106 pKa = 4.16 LDD108 pKa = 3.81 PEE110 pKa = 4.45 EE111 pKa = 4.21 EE112 pKa = 3.85 FLRR115 pKa = 11.84 NYY117 pKa = 10.46 KK118 pKa = 10.04 VVEE121 pKa = 4.78 DD122 pKa = 4.39 GAGHH126 pKa = 7.64 LSTFIEE132 pKa = 4.26 TRR134 pKa = 11.84 AKK136 pKa = 10.27 IEE138 pKa = 3.73 MQIYY142 pKa = 10.12 GDD144 pKa = 4.31 LPMKK148 pKa = 9.84 IWFIFVEE155 pKa = 4.19 QLCRR159 pKa = 11.84 HH160 pKa = 6.16 IGFEE164 pKa = 4.38 TEE166 pKa = 3.76 GLAVMKK172 pKa = 10.68 SFLSKK177 pKa = 10.82 YY178 pKa = 7.19 GTPFHH183 pKa = 6.66 QGLRR187 pKa = 11.84 DD188 pKa = 3.58 LSKK191 pKa = 11.23 LDD193 pKa = 3.89 GFRR196 pKa = 11.84 MSYY199 pKa = 7.72 STPMLFEE206 pKa = 4.12 MSCMEE211 pKa = 4.59 SLLEE215 pKa = 3.84 INIFKK220 pKa = 10.8 RR221 pKa = 11.84 MQEE224 pKa = 3.82 EE225 pKa = 4.7 KK226 pKa = 10.37 IRR228 pKa = 11.84 CLQFGNRR235 pKa = 11.84 NIDD238 pKa = 4.0 PIALLRR244 pKa = 11.84 EE245 pKa = 4.74 FYY247 pKa = 10.52 LICLPHH253 pKa = 7.06 PKK255 pKa = 9.92 RR256 pKa = 11.84 INNILRR262 pKa = 11.84 SPYY265 pKa = 9.91 SWFVKK270 pKa = 8.71 TWGVAADD277 pKa = 4.48 PIVVLKK283 pKa = 11.04 SSGGDD288 pKa = 3.27 DD289 pKa = 3.51 RR290 pKa = 11.84 NSKK293 pKa = 10.17 DD294 pKa = 3.18 VYY296 pKa = 10.11 YY297 pKa = 10.84 EE298 pKa = 3.56 KK299 pKa = 10.66 FQYY302 pKa = 10.54 VEE304 pKa = 3.74 NSYY307 pKa = 11.22 VPIFDD312 pKa = 3.21 ARR314 pKa = 11.84 FYY316 pKa = 11.16 QKK318 pKa = 10.76 SLEE321 pKa = 4.34 DD322 pKa = 3.41 NVEE325 pKa = 4.14 KK326 pKa = 11.06 VNEE329 pKa = 4.52 AIEE332 pKa = 4.07 YY333 pKa = 10.01 SRR335 pKa = 11.84 EE336 pKa = 3.96 LGQHH340 pKa = 6.82 DD341 pKa = 4.1 EE342 pKa = 4.19 GLPIFMSMLKK352 pKa = 10.26 DD353 pKa = 3.48 VYY355 pKa = 9.76 RR356 pKa = 11.84 TPFVPNKK363 pKa = 9.24 PSNLILASFMLSIQTITGYY382 pKa = 10.39 GRR384 pKa = 11.84 AWVKK388 pKa = 10.81 NVGTDD393 pKa = 3.62 FGKK396 pKa = 10.27 QKK398 pKa = 10.64 RR399 pKa = 11.84 PSKK402 pKa = 10.96 DD403 pKa = 3.02 NFIEE407 pKa = 4.15 RR408 pKa = 11.84 VSDD411 pKa = 3.44 YY412 pKa = 9.97 TANNFIAAYY421 pKa = 9.4 EE422 pKa = 4.21 EE423 pKa = 4.3 ARR425 pKa = 11.84 QHH427 pKa = 6.46 HH428 pKa = 6.7 EE429 pKa = 4.76 DD430 pKa = 3.13 IVFPEE435 pKa = 4.19 DD436 pKa = 4.58 LYY438 pKa = 11.27 TSMLRR443 pKa = 11.84 LARR446 pKa = 11.84 NTSSGFSTSVKK457 pKa = 9.31 VRR459 pKa = 11.84 KK460 pKa = 9.9 AFGPNVKK467 pKa = 10.06 RR468 pKa = 11.84 KK469 pKa = 9.7 PEE471 pKa = 4.82 LIDD474 pKa = 3.04 VTSRR478 pKa = 11.84 IKK480 pKa = 11.02 ALVIFTKK487 pKa = 10.2 GHH489 pKa = 4.59 TVFTPEE495 pKa = 4.34 EE496 pKa = 3.86 LNKK499 pKa = 10.37 RR500 pKa = 11.84 YY501 pKa = 7.86 NTTTNYY507 pKa = 5.56 QTKK510 pKa = 9.9 GSRR513 pKa = 11.84 EE514 pKa = 3.95 VPIKK518 pKa = 9.73 ATRR521 pKa = 11.84 TIYY524 pKa = 10.57 SINLSVLVPQLVVTLPLNEE543 pKa = 3.69 YY544 pKa = 8.82 FARR547 pKa = 11.84 VGGSTSPYY555 pKa = 7.63 CKK557 pKa = 10.44 KK558 pKa = 10.01 MGGKK562 pKa = 9.59 IIVGDD567 pKa = 4.15 LEE569 pKa = 4.43 ATGSRR574 pKa = 11.84 VMDD577 pKa = 4.22 AADD580 pKa = 3.62 TFRR583 pKa = 11.84 NSADD587 pKa = 3.3 PGTFTLAIDD596 pKa = 3.5 YY597 pKa = 10.86 SEE599 pKa = 5.11 YY600 pKa = 9.78 DD601 pKa = 3.31 THH603 pKa = 6.43 LTRR606 pKa = 11.84 HH607 pKa = 5.51 NFRR610 pKa = 11.84 GGMIKK615 pKa = 10.49 GLRR618 pKa = 11.84 AAMAKK623 pKa = 9.55 YY624 pKa = 10.31 RR625 pKa = 11.84 NLRR628 pKa = 11.84 YY629 pKa = 9.6 EE630 pKa = 4.6 GYY632 pKa = 10.55 SLDD635 pKa = 3.67 EE636 pKa = 5.44 LIDD639 pKa = 3.68 FGYY642 pKa = 11.11 GDD644 pKa = 4.23 GRR646 pKa = 11.84 VAMTLWNGKK655 pKa = 9.0 RR656 pKa = 11.84 RR657 pKa = 11.84 VFKK660 pKa = 11.11 VPAHH664 pKa = 6.37 LYY666 pKa = 10.53 IMLNEE671 pKa = 4.79 DD672 pKa = 4.13 DD673 pKa = 5.11 RR674 pKa = 11.84 KK675 pKa = 10.75 QGDD678 pKa = 4.15 FKK680 pKa = 10.78 PPPGVRR686 pKa = 11.84 PVTSMAVAKK695 pKa = 10.53 KK696 pKa = 10.36 LIQEE700 pKa = 4.12 KK701 pKa = 10.67 SITDD705 pKa = 3.72 DD706 pKa = 4.18 YY707 pKa = 11.6 ILVSPTDD714 pKa = 3.73 GSDD717 pKa = 3.01 LALIDD722 pKa = 3.54 THH724 pKa = 8.54 LSGEE728 pKa = 4.13 NSTLIANSMHH738 pKa = 5.94 NMAIGTIIQEE748 pKa = 4.21 EE749 pKa = 4.71 VLRR752 pKa = 11.84 KK753 pKa = 9.85 YY754 pKa = 8.83 PTEE757 pKa = 3.5 IAFQSEE763 pKa = 5.03 QYY765 pKa = 11.16 VGDD768 pKa = 3.75 DD769 pKa = 3.37 TLLYY773 pKa = 9.14 TQLLTRR779 pKa = 11.84 DD780 pKa = 3.85 PKK782 pKa = 10.58 VVNGIIEE789 pKa = 4.35 TTFTSVEE796 pKa = 4.21 KK797 pKa = 10.59 CGHH800 pKa = 5.73 EE801 pKa = 3.94 ASASKK806 pKa = 9.91 TMVYY810 pKa = 9.66 PYY812 pKa = 10.42 SVEE815 pKa = 3.92 KK816 pKa = 9.35 TQTHH820 pKa = 6.7 AKK822 pKa = 8.83 QGVYY826 pKa = 10.11 VPQDD830 pKa = 2.61 RR831 pKa = 11.84 MMIISSEE838 pKa = 3.86 RR839 pKa = 11.84 RR840 pKa = 11.84 KK841 pKa = 10.73 DD842 pKa = 3.33 IEE844 pKa = 4.13 NVNGYY849 pKa = 7.96 MRR851 pKa = 11.84 SQVHH855 pKa = 4.65 TMVTKK860 pKa = 9.24 VSRR863 pKa = 11.84 GFSHH867 pKa = 7.84 DD868 pKa = 3.36 LAQWILMLKK877 pKa = 8.78 TVFVGAWKK885 pKa = 10.55 LKK887 pKa = 8.89 RR888 pKa = 11.84 TIKK891 pKa = 10.36 DD892 pKa = 3.01 VHH894 pKa = 6.53 GYY896 pKa = 9.74 RR897 pKa = 11.84 DD898 pKa = 3.28 RR899 pKa = 11.84 KK900 pKa = 10.22 FDD902 pKa = 4.91 DD903 pKa = 4.09 DD904 pKa = 4.99 AEE906 pKa = 5.3 DD907 pKa = 4.13 GFTLVTLKK915 pKa = 10.99 NPLALHH921 pKa = 7.04 IPQSWGGYY929 pKa = 7.11 GAHH932 pKa = 6.95 PAALNIVMTEE942 pKa = 3.81 EE943 pKa = 3.95 MFIDD947 pKa = 3.92 SMQMSRR953 pKa = 11.84 LEE955 pKa = 4.18 VEE957 pKa = 4.25 MQMLTKK963 pKa = 10.42 VAGKK967 pKa = 9.96 LPPAWDD973 pKa = 3.28 EE974 pKa = 4.06 TQADD978 pKa = 3.64 KK979 pKa = 11.21 RR980 pKa = 11.84 QISAEE985 pKa = 3.91 TKK987 pKa = 8.58 MSFFSKK993 pKa = 9.59 MARR996 pKa = 11.84 PAVRR1000 pKa = 11.84 ATLMVPEE1007 pKa = 4.45 IMDD1010 pKa = 3.63 VVEE1013 pKa = 4.42 TLPLGDD1019 pKa = 4.97 FGPGRR1024 pKa = 11.84 LSKK1027 pKa = 9.94 TMMHH1031 pKa = 6.35 SALLKK1036 pKa = 9.33 EE1037 pKa = 4.33 ANARR1041 pKa = 11.84 SLLVSSYY1048 pKa = 9.6 EE1049 pKa = 4.57 LEE1051 pKa = 4.15 YY1052 pKa = 10.8 QKK1054 pKa = 10.83 QLNGWRR1060 pKa = 11.84 EE1061 pKa = 3.86 LPMSFTLDD1069 pKa = 3.41 EE1070 pKa = 4.26 EE1071 pKa = 4.44 SGYY1074 pKa = 11.07 VSSKK1078 pKa = 7.27 YY1079 pKa = 11.22 AKK1081 pKa = 10.02 MFDD1084 pKa = 2.93 IYY1086 pKa = 10.66 FDD1088 pKa = 3.76 EE1089 pKa = 5.35 EE1090 pKa = 4.43 IIEE1093 pKa = 4.36 PAHH1096 pKa = 6.19 VFPDD1100 pKa = 3.77 QNLSPQFYY1108 pKa = 8.4 VQKK1111 pKa = 10.87 AIIGQRR1117 pKa = 11.84 KK1118 pKa = 5.02 STRR1121 pKa = 11.84 MRR1123 pKa = 11.84 MSYY1126 pKa = 10.02 IDD1128 pKa = 4.8 RR1129 pKa = 11.84 IDD1131 pKa = 3.84 SILRR1135 pKa = 11.84 KK1136 pKa = 9.69 DD1137 pKa = 3.48 VVMRR1141 pKa = 11.84 GFLTANTIVNVLEE1154 pKa = 4.15 KK1155 pKa = 11.04 VGISHH1160 pKa = 6.75 TAVDD1164 pKa = 4.8 LVTLFTLMNIEE1175 pKa = 4.04 VKK1177 pKa = 10.55 VAEE1180 pKa = 4.48 EE1181 pKa = 3.58 LAEE1184 pKa = 4.37 YY1185 pKa = 8.54 ITSEE1189 pKa = 4.1 RR1190 pKa = 11.84 VRR1192 pKa = 11.84 FDD1194 pKa = 3.33 AVKK1197 pKa = 10.21 LLKK1200 pKa = 10.44 KK1201 pKa = 10.59 GIVGDD1206 pKa = 4.18 EE1207 pKa = 4.08 FSMSLDD1213 pKa = 3.2 VATQDD1218 pKa = 5.24 MIDD1221 pKa = 3.25 QLIRR1225 pKa = 11.84 YY1226 pKa = 6.75 PHH1228 pKa = 6.93 EE1229 pKa = 3.98 LTKK1232 pKa = 10.77 TEE1234 pKa = 4.13 LDD1236 pKa = 3.46 AVSLYY1241 pKa = 10.56 VSQLIMLRR1249 pKa = 11.84 AALGMKK1255 pKa = 9.57 KK1256 pKa = 9.92 RR1257 pKa = 11.84 RR1258 pKa = 11.84 IRR1260 pKa = 11.84 ISISSDD1266 pKa = 2.48 EE1267 pKa = 4.34 RR1268 pKa = 11.84 EE1269 pKa = 3.85 RR1270 pKa = 11.84 FRR1272 pKa = 11.84 AKK1274 pKa = 8.51 IQRR1277 pKa = 11.84 YY1278 pKa = 6.54 KK1279 pKa = 9.7 THH1281 pKa = 5.86 TPKK1284 pKa = 10.92 LKK1286 pKa = 10.52 LIKK1289 pKa = 10.24 KK1290 pKa = 9.93 LIDD1293 pKa = 3.21 INRR1296 pKa = 11.84 LSVRR1300 pKa = 11.84 ALEE1303 pKa = 4.21 NQFVV1307 pKa = 3.34
Molecular weight: 150.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.001
IPC2_protein 8.141
IPC_protein 8.009
Toseland 8.565
ProMoST 8.536
Dawson 8.96
Bjellqvist 8.799
Wikipedia 9.121
Rodwell 9.092
Grimsley 8.975
Solomon 9.004
Lehninger 8.946
Nozaki 8.814
DTASelect 8.741
Thurlkill 8.829
EMBOSS 9.077
Sillero 8.99
Patrickios 4.571
IPC_peptide 8.99
IPC2_peptide 7.41
IPC2.peptide.svr19 7.566
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
6175
242
1307
617.5
70.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.012 ± 0.634
1.101 ± 0.283
6.04 ± 0.357
7.725 ± 0.501
3.919 ± 0.329
5.992 ± 0.376
2.17 ± 0.286
6.413 ± 0.323
5.846 ± 0.745
8.599 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.676 ± 0.318
4.032 ± 0.257
3.87 ± 0.328
3.271 ± 0.346
7.158 ± 0.385
5.7 ± 0.387
5.344 ± 0.352
7.142 ± 0.371
1.409 ± 0.242
3.579 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here