Sediminibacterium ginsengisoli
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3581 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4LGF0|A0A1T4LGF0_9BACT Uncharacterized membrane protein YgdD TMEM256/DUF423 family OS=Sediminibacterium ginsengisoli OX=413434 GN=SAMN04488132_102486 PE=3 SV=1
MM1 pKa = 7.73 WIVLYY6 pKa = 10.94 LGTNRR11 pKa = 11.84 NFMQPQPLIEE21 pKa = 4.74 LNSSGAAVIAWQLFLQKK38 pKa = 10.48 NGYY41 pKa = 8.54 NVSTADD47 pKa = 3.97 GNFGPQTQAATMAFQTKK64 pKa = 10.4 NGLTADD70 pKa = 3.97 GVVGPGTYY78 pKa = 8.78 TVAEE82 pKa = 4.11 QQGFTGTAVQMADD95 pKa = 3.33 FVGIDD100 pKa = 3.38 VAHH103 pKa = 6.72 YY104 pKa = 10.41 DD105 pKa = 4.13 SNDD108 pKa = 3.2 GANPINWEE116 pKa = 4.23 LVKK119 pKa = 10.37 QDD121 pKa = 3.41 PQNIKK126 pKa = 10.39 FVFVKK131 pKa = 10.02 ATDD134 pKa = 3.88 GASGPNSVDD143 pKa = 2.89 QTFATNVAGAGAVGLPVGAYY163 pKa = 9.42 HH164 pKa = 7.46 FYY166 pKa = 10.89 EE167 pKa = 4.92 LGSAATAQASNFITNTGNHH186 pKa = 5.81 QLLTLPPVLDD196 pKa = 3.97 FEE198 pKa = 5.29 LPVAASAIQQTMDD211 pKa = 6.18 DD212 pKa = 3.55 INTWLEE218 pKa = 3.93 QVEE221 pKa = 4.41 KK222 pKa = 10.99 AFGKK226 pKa = 8.79 TPIIYY231 pKa = 7.81 TNPNAWDD238 pKa = 3.59 ALGNPSGFSKK248 pKa = 10.89 YY249 pKa = 9.76 PLWIADD255 pKa = 4.09 YY256 pKa = 9.71 NSSIAPPVLGSWPQWAFWQFTEE278 pKa = 4.17 TGTVQGIVNPASMDD292 pKa = 3.3 INHH295 pKa = 6.35 YY296 pKa = 10.27 NPASGILAVV305 pKa = 3.98
Molecular weight: 32.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.93
IPC2_protein 4.215
IPC_protein 4.177
Toseland 3.973
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.088
Rodwell 3.999
Grimsley 3.884
Solomon 4.151
Lehninger 4.113
Nozaki 4.279
DTASelect 4.507
Thurlkill 4.012
EMBOSS 4.101
Sillero 4.291
Patrickios 1.977
IPC_peptide 4.151
IPC2_peptide 4.279
IPC2.peptide.svr19 4.189
Protein with the highest isoelectric point:
>tr|A0A1T4M5K7|A0A1T4M5K7_9BACT Gliding motility-associated protein GldC OS=Sediminibacterium ginsengisoli OX=413434 GN=SAMN04488132_103169 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPHH8 pKa = 4.67 KK9 pKa = 9.7 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.4 SVHH16 pKa = 4.97 GFRR19 pKa = 11.84 KK20 pKa = 10.08 RR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 9.14 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.04 GRR39 pKa = 11.84 KK40 pKa = 8.7 KK41 pKa = 10.08 LTVSSEE47 pKa = 4.1 KK48 pKa = 10.7 GLKK51 pKa = 9.34
Molecular weight: 5.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.179
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3581
0
3581
1239871
30
3257
346.2
38.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.23 ± 0.046
0.883 ± 0.014
5.155 ± 0.029
5.587 ± 0.052
4.669 ± 0.034
6.926 ± 0.043
1.895 ± 0.023
6.763 ± 0.035
6.487 ± 0.049
9.441 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.466 ± 0.023
5.229 ± 0.051
3.918 ± 0.029
3.883 ± 0.029
4.551 ± 0.03
6.283 ± 0.042
5.994 ± 0.076
6.447 ± 0.037
1.214 ± 0.015
3.981 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here