Halohasta litchfieldiae

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halohasta

Average proteome isoelectric point is 4.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3425 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6RCS2|A0A1H6RCS2_9EURY Adenylate cyclase OS=Halohasta litchfieldiae OX=1073996 GN=SAMN05444271_10298 PE=4 SV=1
MM1 pKa = 7.13SQDD4 pKa = 2.82ICVMVPTIRR13 pKa = 11.84EE14 pKa = 4.13YY15 pKa = 11.0EE16 pKa = 4.16CMRR19 pKa = 11.84SYY21 pKa = 10.53FQNARR26 pKa = 11.84DD27 pKa = 3.62HH28 pKa = 7.2DD29 pKa = 4.42FDD31 pKa = 4.47LSRR34 pKa = 11.84LHH36 pKa = 6.64VVLITEE42 pKa = 4.84DD43 pKa = 3.61FCDD46 pKa = 3.83TDD48 pKa = 3.52EE49 pKa = 4.3MQAMLDD55 pKa = 3.66EE56 pKa = 4.93EE57 pKa = 5.08GVDD60 pKa = 3.83GEE62 pKa = 4.54VFDD65 pKa = 4.37GSRR68 pKa = 11.84RR69 pKa = 11.84EE70 pKa = 4.1EE71 pKa = 3.86WFEE74 pKa = 3.65AHH76 pKa = 7.12DD77 pKa = 3.75VSEE80 pKa = 4.35YY81 pKa = 10.48SHH83 pKa = 6.78IVPAASHH90 pKa = 6.76AEE92 pKa = 4.07TSFGLLYY99 pKa = 9.72MWAGDD104 pKa = 3.51YY105 pKa = 10.91DD106 pKa = 4.12YY107 pKa = 11.67GFFIDD112 pKa = 6.19DD113 pKa = 3.76DD114 pKa = 4.37TLPHH118 pKa = 7.2DD119 pKa = 3.94EE120 pKa = 5.32DD121 pKa = 4.44FFGTHH126 pKa = 5.56MEE128 pKa = 3.99NLDD131 pKa = 3.65FEE133 pKa = 5.1GEE135 pKa = 4.16IEE137 pKa = 4.41SVSSDD142 pKa = 3.71EE143 pKa = 3.72QWVNVLYY150 pKa = 11.0QNAEE154 pKa = 3.85EE155 pKa = 4.46HH156 pKa = 6.72DD157 pKa = 4.72LYY159 pKa = 10.74PRR161 pKa = 11.84GYY163 pKa = 8.97PYY165 pKa = 11.08SAMHH169 pKa = 6.41EE170 pKa = 4.38SVEE173 pKa = 4.37TEE175 pKa = 3.83TTEE178 pKa = 3.54VDD180 pKa = 3.97NIVASQGLWTNVPDD194 pKa = 4.12LDD196 pKa = 4.07AVRR199 pKa = 11.84ILMDD203 pKa = 4.92GDD205 pKa = 4.0LQGQAQTRR213 pKa = 11.84TTRR216 pKa = 11.84EE217 pKa = 3.87DD218 pKa = 3.54FEE220 pKa = 6.44GDD222 pKa = 3.59FVAAEE227 pKa = 4.08DD228 pKa = 4.31NYY230 pKa = 10.86LTVCSMNLAFEE241 pKa = 4.55RR242 pKa = 11.84EE243 pKa = 4.45VIPAFYY249 pKa = 9.9QLPMDD254 pKa = 4.1EE255 pKa = 4.94NEE257 pKa = 3.9WDD259 pKa = 3.27VGRR262 pKa = 11.84FDD264 pKa = 6.36DD265 pKa = 3.27IWSGVFLKK273 pKa = 10.5RR274 pKa = 11.84ACDD277 pKa = 3.42ILDD280 pKa = 3.44KK281 pKa = 10.88QIYY284 pKa = 9.67NGLPLCEE291 pKa = 4.46HH292 pKa = 6.47NKK294 pKa = 10.1APRR297 pKa = 11.84STFDD301 pKa = 3.64DD302 pKa = 4.25LNNEE306 pKa = 4.23VPGLEE311 pKa = 4.22LNEE314 pKa = 4.33HH315 pKa = 5.94LWEE318 pKa = 4.66IVDD321 pKa = 4.41EE322 pKa = 4.31VDD324 pKa = 5.05PDD326 pKa = 4.53LSDD329 pKa = 5.26ADD331 pKa = 4.13DD332 pKa = 4.33PYY334 pKa = 12.07ARR336 pKa = 11.84IFAAMAEE343 pKa = 4.13EE344 pKa = 4.54LADD347 pKa = 4.37GDD349 pKa = 3.28WSEE352 pKa = 4.35YY353 pKa = 10.71NNGDD357 pKa = 3.32FFNYY361 pKa = 9.38IGEE364 pKa = 4.46FMGDD368 pKa = 2.91WLDD371 pKa = 3.68CLEE374 pKa = 5.21GIEE377 pKa = 4.7QAAEE381 pKa = 3.53PAANRR386 pKa = 11.84RR387 pKa = 11.84VV388 pKa = 3.2

Molecular weight: 44.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6TYJ6|A0A1H6TYJ6_9EURY Xaa-Pro aminopeptidase OS=Halohasta litchfieldiae OX=1073996 GN=SAMN05444271_10959 PE=4 SV=1
MM1 pKa = 6.94THH3 pKa = 6.1TAIKK7 pKa = 9.46RR8 pKa = 11.84QKK10 pKa = 10.09FVIRR14 pKa = 11.84HH15 pKa = 4.72LRR17 pKa = 11.84SVNSLYY23 pKa = 10.93AQLVDD28 pKa = 3.42AVVGFGCEE36 pKa = 3.63VDD38 pKa = 3.68GVLQIRR44 pKa = 11.84PKK46 pKa = 10.49RR47 pKa = 3.66

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3425

0

3425

947826

24

1710

276.7

30.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.719 ± 0.045

0.76 ± 0.015

8.214 ± 0.05

8.438 ± 0.055

3.309 ± 0.031

7.975 ± 0.042

1.997 ± 0.021

5.037 ± 0.035

2.251 ± 0.032

8.943 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.786 ± 0.018

2.694 ± 0.028

4.488 ± 0.03

3.157 ± 0.028

5.915 ± 0.041

6.259 ± 0.036

6.901 ± 0.039

8.367 ± 0.04

1.093 ± 0.016

2.696 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski