Strawberry polerovirus 1
Average proteome isoelectric point is 7.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097RN44|A0A097RN44_9LUTE Readthrough protein OS=Strawberry polerovirus 1 OX=1564903 PE=3 SV=1
MM1 pKa = 7.38 FEE3 pKa = 4.27 VKK5 pKa = 10.45 FGYY8 pKa = 9.97 LVPTLFEE15 pKa = 4.16 VTDD18 pKa = 3.55 LHH20 pKa = 7.23 DD21 pKa = 3.99 FADD24 pKa = 3.93 AFDD27 pKa = 4.13 AFEE30 pKa = 4.07 GLRR33 pKa = 11.84 FHH35 pKa = 7.52 ALLQEE40 pKa = 3.83 VRR42 pKa = 11.84 KK43 pKa = 9.05 YY44 pKa = 11.14 NRR46 pKa = 11.84 VGFSRR51 pKa = 11.84 SNYY54 pKa = 10.06 AVDD57 pKa = 3.91 RR58 pKa = 11.84 DD59 pKa = 3.84 VNLAFIFYY67 pKa = 10.37 LPLLTQPGNKK77 pKa = 8.47 PQPGDD82 pKa = 3.7 SFSVPLARR90 pKa = 11.84 YY91 pKa = 8.02 KK92 pKa = 10.51 QLIKK96 pKa = 9.76 WALAVGYY103 pKa = 9.67 FPEE106 pKa = 4.76 LRR108 pKa = 11.84 EE109 pKa = 4.41 LPTTVQVCCTHH120 pKa = 7.17 RR121 pKa = 11.84 STEE124 pKa = 3.4 TDD126 pKa = 3.08 YY127 pKa = 11.25 RR128 pKa = 11.84 RR129 pKa = 11.84 KK130 pKa = 10.13 LWEE133 pKa = 4.92 LDD135 pKa = 3.47 TADD138 pKa = 3.28 VAKK141 pKa = 10.81 DD142 pKa = 3.56 VVGCKK147 pKa = 9.82 HH148 pKa = 6.48 LPLLGANTLANIMCQRR164 pKa = 11.84 FKK166 pKa = 11.25 LLQDD170 pKa = 3.2 EE171 pKa = 4.36 HH172 pKa = 8.08 RR173 pKa = 11.84 KK174 pKa = 9.15 IVPITRR180 pKa = 11.84 NARR183 pKa = 11.84 FEE185 pKa = 3.98 VHH187 pKa = 6.9 HH188 pKa = 6.33 IHH190 pKa = 7.21 LVLCLKK196 pKa = 10.85 DD197 pKa = 4.49 DD198 pKa = 3.71 MGFLMEE204 pKa = 5.42 FDD206 pKa = 4.55 YY207 pKa = 11.3 EE208 pKa = 4.09 PHH210 pKa = 6.44 CRR212 pKa = 11.84 RR213 pKa = 11.84 FTLDD217 pKa = 2.86 VARR220 pKa = 11.84 RR221 pKa = 11.84 LHH223 pKa = 6.42 GFGFQRR229 pKa = 11.84 SAMDD233 pKa = 3.03 IFEE236 pKa = 4.37 TSFLRR241 pKa = 11.84 SDD243 pKa = 2.86 ITMEE247 pKa = 4.39 DD248 pKa = 3.4 LEE250 pKa = 4.56 AFYY253 pKa = 10.42 PP254 pKa = 3.92
Molecular weight: 29.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.034
IPC2_protein 5.97
IPC_protein 6.059
Toseland 6.427
ProMoST 6.465
Dawson 6.389
Bjellqvist 6.364
Wikipedia 6.415
Rodwell 6.376
Grimsley 6.561
Solomon 6.389
Lehninger 6.389
Nozaki 6.664
DTASelect 6.854
Thurlkill 6.868
EMBOSS 6.839
Sillero 6.781
Patrickios 4.177
IPC_peptide 6.402
IPC2_peptide 6.737
IPC2.peptide.svr19 6.67
Protein with the highest isoelectric point:
>tr|A0A097RN38|A0A097RN38_9LUTE p0 OS=Strawberry polerovirus 1 OX=1564903 PE=4 SV=1
MM1 pKa = 7.54 ARR3 pKa = 11.84 RR4 pKa = 11.84 PRR6 pKa = 11.84 GSRR9 pKa = 11.84 GGKK12 pKa = 9.11 GKK14 pKa = 10.44 KK15 pKa = 8.92 KK16 pKa = 10.24 QNAGRR21 pKa = 11.84 RR22 pKa = 11.84 NPSRR26 pKa = 11.84 MARR29 pKa = 11.84 PAQPIYY35 pKa = 10.63 VVQRR39 pKa = 11.84 PPNQGTGRR47 pKa = 11.84 RR48 pKa = 11.84 GRR50 pKa = 11.84 RR51 pKa = 11.84 NRR53 pKa = 11.84 NRR55 pKa = 11.84 RR56 pKa = 11.84 LRR58 pKa = 11.84 AQGYY62 pKa = 8.54 GLGGKK67 pKa = 9.33 HH68 pKa = 5.34 EE69 pKa = 4.31 TLKK72 pKa = 9.85 FTKK75 pKa = 10.25 EE76 pKa = 3.82 DD77 pKa = 3.04 IKK79 pKa = 11.14 RR80 pKa = 11.84 NSSGYY85 pKa = 7.97 ITFGPSLSEE94 pKa = 4.34 HH95 pKa = 6.52 PAFSNGNLKK104 pKa = 10.32 AYY106 pKa = 9.04 KK107 pKa = 9.45 HH108 pKa = 5.09 YY109 pKa = 10.53 RR110 pKa = 11.84 ITRR113 pKa = 11.84 INVEE117 pKa = 4.24 YY118 pKa = 10.49 LSEE121 pKa = 4.3 STDD124 pKa = 3.52 QAPGAIKK131 pKa = 10.63 FEE133 pKa = 4.39 MDD135 pKa = 3.0 TSLSATKK142 pKa = 9.19 VTSPIHH148 pKa = 6.15 RR149 pKa = 11.84 FPIKK153 pKa = 10.54 KK154 pKa = 9.58 NGRR157 pKa = 11.84 YY158 pKa = 6.96 TWTAPHH164 pKa = 6.97 INGQLTRR171 pKa = 11.84 EE172 pKa = 4.29 TTSDD176 pKa = 2.92 QCRR179 pKa = 11.84 FLYY182 pKa = 10.42 EE183 pKa = 3.8 ATTTSTEE190 pKa = 3.82 PAGVFNFTYY199 pKa = 10.2 DD200 pKa = 3.29 VHH202 pKa = 8.64 LMDD205 pKa = 5.52 NKK207 pKa = 10.96
Molecular weight: 23.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.999
IPC_protein 10.906
Toseland 10.95
ProMoST 10.789
Dawson 11.038
Bjellqvist 10.789
Wikipedia 11.286
Rodwell 11.228
Grimsley 11.082
Solomon 11.199
Lehninger 11.155
Nozaki 10.921
DTASelect 10.789
Thurlkill 10.95
EMBOSS 11.374
Sillero 10.965
Patrickios 10.935
IPC_peptide 11.213
IPC2_peptide 9.765
IPC2.peptide.svr19 8.326
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3155
185
1139
525.8
59.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.498 ± 0.39
1.585 ± 0.32
4.913 ± 0.435
6.022 ± 0.299
3.74 ± 0.534
5.769 ± 0.368
2.536 ± 0.188
4.976 ± 0.443
5.547 ± 0.31
8.336 ± 1.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.87 ± 0.178
4.216 ± 0.33
6.973 ± 0.715
3.518 ± 0.459
6.815 ± 0.857
9.002 ± 0.901
6.656 ± 0.596
5.42 ± 0.608
1.902 ± 0.228
3.708 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here